Effect of Partial Hydrolysis with Papain on the Characteristics of Transglutaminase-Crosslinked Tofu Gel.

The effects of partial enzymatic hydrolysis of soymilk on the characteristics of transglutaminase (TG)-crosslinked tofu gel were studied. SDS-PAGE showed that the molecular weight of the partially hydrolyzed soybean protein was reduced to that of a digested peptide (less than 43.0 kDa) when papain was added at more than 50 μL/100 mL soymilk. The content of free sulfhydryls, β-sheets, and random coils in papain-treated soymilk increased. When TG was added to soy milk after papain treatment and tofu gel was formed, its storage modulus increased from 957.44 to 1241.39 Pa. The gel strength, water-holding capacity, and nonfreezing water content of the tofu gel were greater than those without enzyme treatment. Scanning electron microscopy revealed that limited papain hydrolysis stimulated TG-catalyzed cross-linking of soymilk to form a dense gel network structure, whereas an extended enzymatic hydrolysis of soymilk did not promote crosslinking by TG. PRACTICAL APPLICATION: This work investigated the effect of partial hydrolysis on TG cross-linked tofu gel. Partial hydrolysis of soybean protein with papain can promote TG cross-linking reaction, thus form a dense network structure, increase gel strength, and water-holding capacity. Therefore, it can be used to produce a good gel product with higher gel strength, springiness, water-holding capacity, and a more dense microstructure.

[1]  Shaotong Jiang,et al.  Effects of organic acid coagulants on the physical properties of and chemical interactions in tofu , 2017 .

[2]  R. Kita,et al.  Physical properties of tofu gel probed by water translational/rotational dynamics , 2017 .

[3]  A. Gillen,et al.  Protein and quality analyses of accessions from the USDA soybean germplasm collection for tofu production. , 2016, Food chemistry.

[4]  Wei Li,et al.  A comparison study of bioaccessibility of soy protein gel induced by magnesiumchloride, glucono-δ-lactone and microbial transglutaminase , 2016 .

[5]  Shaotong Jiang,et al.  Transglutaminase-induced gelation properties of soy protein isolate and wheat gluten mixtures with high intensity ultrasonic pretreatment. , 2016, Ultrasonics sonochemistry.

[6]  Lijun Yin,et al.  Effect of magnesium salt concentration in water-in-oil emulsions on the physical properties and microstructure of tofu. , 2016, Food chemistry.

[7]  Shaotong Jiang,et al.  Effects of partial hydrolysis and subsequent cross-linking on wheat gluten physicochemical properties and structure. , 2016, Food chemistry.

[8]  Hongshun Yang,et al.  Preparation of organic tofu using organic compatible magnesium chloride incorporated with polysaccharide coagulants. , 2015, Food chemistry.

[9]  B. Adhikari,et al.  Effect of gums on the rheological characteristics and microstructure of acid-induced SPI-gum mixed gels. , 2014, Carbohydrate polymers.

[10]  M. Añón,et al.  Incorporation of Lactobacillus delbrueckii subsp lactis (CIDCA 133) in cold-set gels made from high pressure-treated soybean proteins , 2014 .

[11]  Zhongjiang Wang,et al.  Relationship between Secondary Structure and Surface Hydrophobicity of Soybean Protein Isolate Subjected to Heat Treatment , 2014 .

[12]  Wang Shengnan,et al.  HPLC Analysis of Water-Soluble Vitamins (B2, B3, B6, B12, and C) and Fat-Soluble Vitamins (E, K, D, A, and β-Carotene) of Okra (Abelmoschus esculentus) , 2014 .

[13]  Qi Zhang,et al.  Effects of different coagulants on coagulation behavior of acid-induced soymilk , 2013 .

[14]  H. Mo,et al.  Effects of Pulsed Electric Field Processing on Quality Characteristics and Microbial Inactivation of Soymilk , 2013, Food and Bioprocess Technology.

[15]  Y. Xiong,et al.  Extreme pH treatments enhance the structure-reinforcement role of soy protein isolate and its emulsions in pork myofibrillar protein gels in the presence of microbial transglutaminase. , 2013, Meat science.

[16]  Jiaoyan Ren,et al.  Enzymatic hydrolysis and their effects on conformational and functional properties of peanut protein isolate , 2011 .

[17]  Yung-Ho Chang,et al.  Effect of microbial transglutaminase on the rheological and textural characteristics of black soybean packed tofu coagulating with Agar , 2011 .

[18]  Bo Zhang,et al.  Characterization of water state and distribution in textured soybean protein using DSC and NMR , 2010 .

[19]  X. Sun,et al.  Gelation properties of salt-extracted pea protein induced by heat treatment , 2010 .

[20]  Y. Xiong,et al.  Effect of soy protein substitution for sodium caseinate on the transglutaminate-induced cold and thermal gelation of myofibrillar protein☆ , 2009 .

[21]  M. Corredig,et al.  Protein Subunit Composition Effects on the Thermal Denaturation at Different Stages During the Soy Protein Isolate Processing and Gelation Profiles of Soy Protein Isolates , 2008 .

[22]  Xiaoquan Yang,et al.  Formation and rheological properties of ‘cold-set’ tofu induced by microbial transglutaminase , 2007 .

[23]  Xiaoquan Yang,et al.  Improvement on functional properties of wheat gluten by enzymatic hydrolysis and ultrafiltration , 2006 .

[24]  F. Avilés,et al.  Amyloid Fibril Formation by a Partially Structured Intermediate State of α-Chymotrypsin , 2004 .

[25]  Nan-Wei Su,et al.  Effect of calcium sulfate concentration in soymilk on the microstructure of firm tofu and the protein constitutions in tofu whey. , 2003, Journal of agricultural and food chemistry.

[26]  M. Añón,et al.  Structural Properties of Heat-Induced Soy Protein Gels As Affected by Ionic Strength and pH , 1998 .

[27]  A. Kilara,et al.  Enhancing the functionality of food proteins by enzymatic modification , 1996 .

[28]  H. Yoshida,et al.  Characterization of water in polysaccharide hydrogels by DSC , 1993 .

[29]  J. Adler-Nissen,et al.  Enzymic Hydrolysis of Food Proteins , 1986 .

[30]  J. Caygill,et al.  Concentration of plant proteases by precipitation with polyacrylic acids , 1983 .

[31]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[32]  Zhengyu Jin,et al.  Effect of pullulan on the water distribution, microstructure and textural properties of rice starch gels during cold storage. , 2017, Food chemistry.

[33]  S. H. Prudencio,et al.  Effect of soybean aging on the quality of soymilk, firmness of tofu and optimum coagulant concentration. , 2016, Food chemistry.

[34]  J. Cheftel,et al.  Sulfhydryl group/disulfide bond interchange reactions during heat-induced gelation of whey protein isolate , 1989 .

[35]  J. Cheftel,et al.  Determination of sulfhydryl groups and disulfide bonds in heat-induced gels of soy protein isolate , 1988 .