The F286Y mutation of PrlA4 tempers the signal sequence suppressor phenotype by reducing the SecA binding affinity

[1]  A. Driessen,et al.  Mapping the Sites of Interaction between SecY and SecE by Cysteine Scanning Mutagenesis* , 2001, The Journal of Biological Chemistry.

[2]  A. Driessen,et al.  The structural basis of protein targeting and translocation in bacteria , 2001, Nature Structural Biology.

[3]  T. Rapoport,et al.  Projection structure and oligomeric properties of a bacterial core protein translocase , 2001, The EMBO journal.

[4]  A. Engel,et al.  SecYEG assembles into a tetramer to form the active protein translocation channel , 2000, The EMBO journal.

[5]  A. Driessen,et al.  Preprotein Translocation by a Hybrid Translocase Composed of Escherichia coli and Bacillus subtilis Subunits , 1999, Journal of bacteriology.

[6]  A. Driessen,et al.  Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE. , 1999, Biochemistry.

[7]  W. Wickner,et al.  The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits , 1999, The EMBO journal.

[8]  K. Nishiyama,et al.  Membrane deinsertion of SecA underlying proton motive force‐dependent stimulation of protein translocation , 1999, The EMBO journal.

[9]  T. Silhavy,et al.  PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA–SecY interaction during the initiation of translocation , 1998, The EMBO journal.

[10]  A. Driessen,et al.  Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state. , 1998, Biochemistry.

[11]  A. Driessen,et al.  The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events , 1997, The EMBO journal.

[12]  D. Belin,et al.  prl Mutations in the Escherichia coli secG Gene* , 1997, The Journal of Biological Chemistry.

[13]  J. Beckwith,et al.  Targeting of signal sequenceless proteins for export in Escherichia coli with altered protein translocase. , 1996, The EMBO journal.

[14]  J. Barbero,et al.  Different PrlA proteins increase the efficiency of periplasmic production of human interleukin-6 in Escherichia coli. , 1996, Journal of biotechnology.

[15]  J. Tommassen,et al.  prlA suppressors in Escherichia coli relieve the proton electrochemical gradient dependency of translocation of wild-type precursors. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[16]  T. Silhavy,et al.  Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA , 1995, Journal of bacteriology.

[17]  W. Wickner,et al.  SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion , 1994, Cell.

[18]  R. Doebele,et al.  PrlA and PrlG suppressors reduce the requirement for signal sequence recognition , 1994, Journal of bacteriology.

[19]  K. Nishiyama,et al.  A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli. , 1993, The EMBO journal.

[20]  R. S. Osborne,et al.  PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains. , 1993, The EMBO journal.

[21]  J. Beckwith,et al.  A signal sequence is not required for protein export in prlA mutants of Escherichia coli. , 1993, The EMBO journal.

[22]  A. Driessen,et al.  Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive force. , 1992, The EMBO journal.

[23]  W. Wickner,et al.  SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase. , 1992, The Journal of biological chemistry.

[24]  J. Hendrick,et al.  SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membrane. , 1991, The Journal of biological chemistry.

[25]  S. Mizushima,et al.  Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[26]  Arnold J. M. Driessen,et al.  Δμ H+ and ATP function at different steps of the catalytic cycle of preprotein translocase , 1991, Cell.

[27]  M. Inouye,et al.  Effect of OmpA signal peptide mutations on OmpA secretion, synthesis, and assembly , 1991, Journal of bacteriology.

[28]  F. Hartl,et al.  The binding cascade of SecB to SecA to SecY E mediates preprotein targeting to the E. coli plasma membrane , 1990, Cell.

[29]  K. Bieker,et al.  Thesec andprl genes ofEscherichia coli , 1990, Journal of bioenergetics and biomembranes.

[30]  W. Wickner,et al.  The ATPase activity of secA is regulated by acidic phospholipids, secY, and the leader and mature domains of precursor proteins , 1990, Cell.

[31]  W. Wickner,et al.  Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[32]  L. Gansheroff,et al.  New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli. , 1989, Genes & development.

[33]  W. Wickner,et al.  SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA. , 1989, The EMBO journal.

[34]  Koreaki Ito,et al.  SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli. , 1989, The EMBO journal.

[35]  J. Fikes,et al.  Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide , 1989, Journal of bacteriology.

[36]  T. Sako,et al.  Distinct mutation sites in prlA suppressor mutant strains of Escherichia coli respond either to suppression of signal peptide mutations or to blockage of staphylokinase processing , 1988, Journal of bacteriology.

[37]  S. Emr,et al.  Suppressor mutations that restore export of a protein with a defective signal sequence , 1981, Cell.

[38]  A. Driessen,et al.  University of Groningen A single amino acid substitution in SecY stabilizes the interaction with SecA , 1999 .