Changes in pancreatic and intestinal enzyme activities following chenodeoxycholic acid feeding.

The effect of CDCA feeding on pancreatic and intestinal enzymes was studied. Mice were fed 0.5% w/w chenodeoxycholic acid in a normal diet. Pancreatic lipase concentration was significantly increased after 3 days on the CDCA diet, while amylase and trypsin concentrations were significantly higher at 23 days when compared with the controls. At 70 days there was a significant increase in the concentrations of amylase, trypsin, and lipase. Protein concentrations paralleled the rise in enzyme levels. Amylase and lipase, when measured as specific activities, were still higher than the controls at 70 days. Intestinal amylase levels did not change during the experiments, but intestinal alpha-glucosidase activity increased significantly in the CDCA-treated animals. The results are discussed in terms of their similarity with those reported to occur after feeding soybean trypsin inhibitor.

[1]  S. J. Holt,et al.  Hypertrophy and hyperplasia of the neonatal rat exocrine pancreas induced by orally administered soybean trypsin inhibitor. , 1976, Biochimica et biophysica acta.

[2]  I. Ihse.  Abolishment of oral trypsin inhibitor stimulation of the rat exocrine pancreas after duodeno-jejunal resection. , 1976, Scandinavian journal of gastroenterology.

[3]  M. D. O'donnell,et al.  α-Amylase and glucoamylase activities of canine serum☆ , 1975 .

[4]  M. D. O'donnell,et al.  Effect of free and conjugated bile salts on alpha-amylase activity. , 1975, Enzyme.

[5]  R. Dowling,et al.  Effect of different doses of chenodeoxycholic acid on bile-lipid composition and on frequency of side-effects in patients with gallstones. , 1974, Lancet.

[6]  T. Drüeke,et al.  Letter: Chenodeoxycholic acid: possible intestinal injury. , 1974, The New England journal of medicine.

[7]  Fölsch Ur,et al.  The pancreatic secretion of enzymes in rats treated with soybean diet. , 1974 .

[8]  M. D. O'donnell,et al.  Comparison of saccharogenic and PHADEBAS methods for amylase assay in biological fluids. , 1974, Enzyme.

[9]  K. Winckler,et al.  Effect of a soybean diet on enzyme content and ultrastructure of the rat exocrine pancreas. , 1974, Digestion.

[10]  A. Bouchier,et al.  Proceedings: A study of the effectiveness, toxicity, and bile salt kinetics of chenodeoxycholic acid in the treatment of human gallstones. , 1973, Gut.

[11]  J. Thistle,et al.  Efficacy and specificity of chenodeoxycholic acid therapy for dissolving gallstones. , 1973, The New England journal of medicine.

[12]  O. James.  The Lundh test. , 1973, Gut.

[13]  R. Dowling,et al.  Gallstone dissolution in man using chenodeoxycholic acid. , 1972, Lancet.

[14]  R. Lyman,et al.  Feedback Regulation of Pancreatic Enzyme Secretion as a Mechanism for Trypsin Inhibitor-Induced Hypersecretion in Rats 1 , 1972, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[15]  J. Thistle,et al.  Dissolution of cholesterol gallstones by chenodeoxycholic acid. , 1972, The New England journal of medicine.

[16]  D. Small.  Prestone gallstone disease--is therapy safe? , 1971, The New England journal of medicine.

[17]  R. Melmed,et al.  A further physiological role for naturally occurring trypsin inhibitors: the evidence for a trophic stimulant of the pancreatic acinar cell , 1969, Gut.

[18]  R. Lyman,et al.  Growth depression and pancreatic and intestinal changes in rats force-fed amino acid diets containing soybean trypsin inhibitor. , 1966, The Journal of nutrition.

[19]  M. Novak.  COLORIMETRIC ULTRAMICRO METHOD FOR THE DETERMINATION OF FREE FATTY ACIDS. , 1965, Journal of lipid research.

[20]  William W. Cohen,et al.  The preparation and properties of two new chromogenic substrates of trypsin. , 1961, Archives of biochemistry and biophysics.

[21]  D. J. Robbins,et al.  Effect of Raw Soybean Meal and Amino Acids on Pancreatic Hypertrophy in Rats , 1960 .

[22]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[23]  S. Chernick,et al.  A dietary factor regulating the enzyme content of the pancreas; changes induced in size and proteolytic activity of the chick pancreas by the ingestion of raw soy-bean meal. , 1948, The American journal of physiology.