A new species of the genus Rana from Henan, central China (Anura, Ranidae)

Abstract A new species of brown frog Rana luanchuanensis Zhao & Yuan, sp. n. is described from Luanchuan County, western Henan, central China. The mitochondrial genealogy suggests that the new species is the sister taxon to the clade including R. amurensis and R. coreana, and is separated by uncorrected pairwise distances more than 12.5%. Morphologically, this new species differs from its congeners by a suite of characters. Analyses of partial sequences of cytochrome oxidase subunit I (COI) resolve the new species as a single matriline.

[1]  D. Hillis,et al.  Spatiotemporal Diversification of the True Frogs (Genus Rana): A Historical Framework for a Widely Studied Group of Model Organisms. , 2016, Systematic biology.

[2]  P. Hebert,et al.  Assessing DNA Barcodes for Species Identification in North American Reptiles and Amphibians in Natural History Collections , 2016, PloS one.

[3]  F. Glaw,et al.  Comprehensive DNA barcoding of the herpetofauna of Germany , 2016, Molecular ecology resources.

[4]  J. Fong,et al.  Molecular and Morphological Evidence for Rana kunyuensis as a Junior Synonym of Rana coreana (Anura: Ranidae) , 2015 .

[5]  K. Ukena,et al.  Identification of Neurotensin and LANT-6 and Localization of mRNA Encoding Their Precursor in the Chicken Brain , 2014, Zoological science.

[6]  Alexandros Stamatakis,et al.  RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies , 2014, Bioinform..

[7]  Koichiro Tamura,et al.  MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. , 2013, Molecular biology and evolution.

[8]  B. Stuart,et al.  A new tree frog in the genus Polypedates (Anura: Rhacophoridae) from southern Thailand. , 2013, Zootaxa.

[9]  Ramón Doallo,et al.  CircadiOmics: integrating circadian genomics, transcriptomics, proteomics and metabolomics , 2012, Nature Methods.

[10]  R. Murphy,et al.  Universal COI primers for DNA barcoding amphibians , 2012, Molecular ecology resources.

[11]  M. Matsui On the Brown Frogs from the Ryukyu Archipelago, Japan, with Descriptions of Two New Species (Amphibia, Anura) , 2011 .

[12]  R. Murphy,et al.  Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China , 2011 .

[13]  杨军校,et al.  Validity and systematic position of Rana altaica (Rana: ranidae): results of a phylogenetic analysis. , 2010 .

[14]  Ya-ping Zhang,et al.  Phylogeny of Raninae (Anura: Ranidae) inferred from mitochondrial and nuclear sequences. , 2007, Molecular phylogenetics and evolution.

[15]  D. Hillis,et al.  Phylogeny of the New World true frogs (Rana). , 2005, Molecular phylogenetics and evolution.

[16]  Robert C. Edgar,et al.  MUSCLE: multiple sequence alignment with high accuracy and high throughput. , 2004, Nucleic acids research.

[17]  John P. Huelsenbeck,et al.  MrBayes 3: Bayesian phylogenetic inference under mixed models , 2003, Bioinform..

[18]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[19]  S. Kuzmin,et al.  Validity and systematic position of Rana altaica (Rana: ranidae): results of a phylogenetic analysis. , 2010, Dong wu xue yan jiu = Zoological research.

[20]  Ya-ping Zhang,et al.  Phylogenetic Relationships of the Chinese Brown Frogs (Genus Rana) Inferred from Partial Mitochondrial 12S and 16S rRNA Gene Sequences , 2007, Zoological science.

[21]  Lu-zhen Yu A NEW WOOD-FROG OF THE GENUS RANA IN MT.KUNYU, SHANDONG PROVINCE,CHINA (AMPHIBIA:ANURA:RANIDAE) , 2002 .

[22]  G. Boulenger A monograph of the American frogs of the genus Rana , 1920 .