Molecular characterization of the Arabidopsis floral homeotic gene APETALA1

THE first step in flower development is the transition of an inflorescence meristem into a floral meristem. Each floral meristem differentiates into a flower consisting of four organ types that occupy precisely defined positions within four concentric whorls. Genetic studies in Arabidopsis thaliana and Antirrhinum majus have identified early-acting genes that determine the identity of the floral meristem, and late-acting genes that determine floral organ identity1–5. In Arabidopsis, at least two genes, APETALA1 and LEAFY, are required for the transition of an inflorescence meristem into a floral meristem1. We have cloned the APETALA1 gene and here we show that it encodes a putative transcription factor that contains a MADS-domain2. APETALA1 RNA is uniformly expressed in young flower primordia, and later becomes localized to sepals and petals. Our results suggest that APETALA1 acts locally to specify the identity of the floral meristem, and to determine sepal and petal development.

[1]  Richard Treisman,et al.  Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element , 1988, Cell.

[2]  D. Weigel,et al.  LEAFY controls floral meristem identity in Arabidopsis , 1992, Cell.

[3]  H. Sommer,et al.  Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors. , 1990, The EMBO journal.

[4]  W. Nacken,et al.  Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus , 1990, Science.

[5]  E. Meyerowitz,et al.  AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. , 1991, Genes & development.

[6]  H. Sommer,et al.  Bracteomania, an inflorescence anomaly, is caused by the loss of function of the MADS‐box gene squamosa in Antirrhinum majus. , 1992, The EMBO journal.

[7]  A. Nordheim,et al.  A protein domain conserved between yeast MCM1 and human SRF directs ternary complex formation. , 1991, The EMBO journal.

[8]  E. Coen,et al.  floricaula: A homeotic gene required for flower development in antirrhinum majus , 1990, Cell.

[9]  H. Sommer,et al.  Characterization of the Antirrhinum floral homeotic MADS‐box gene deficiens: evidence for DNA binding and autoregulation of its persistent expression throughout flower development. , 1992, The EMBO journal.

[10]  J. Bowman,et al.  Genetic interactions among floral homeotic genes of Arabidopsis. , 1991, Development.

[11]  Elliot M. Meyerowitz,et al.  The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens , 1992, Cell.

[12]  R. Elble,et al.  Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MAT alpha cells. , 1988, Journal of molecular biology.

[13]  R. W. Davis,et al.  Nitrate reductase from squash: cDNA cloning and nitrate regulation. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Hong Ma,et al.  The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors , 1990, Nature.

[15]  J. Bowman,et al.  Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product , 1991, Cell.

[16]  Maarten Koornneef,et al.  Linkage map of Arabidopsis thaliana , 1983 .

[17]  J. Bowman,et al.  Early flower development in Arabidopsis. , 1990, The Plant cell.

[18]  I. Sussex,et al.  Function of the apetala-1 gene during Arabidopsis floral development. , 1990, The Plant cell.

[19]  P M Steinert,et al.  Molecular and cellular biology of intermediate filaments. , 1988, Annual review of biochemistry.

[20]  E S Lander,et al.  Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[21]  R. W. Davis,et al.  Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[22]  B. Cochran,et al.  The human c-fos serum response factor and the yeast factors GRM/PRTF have related DNA-binding specificities. , 1988, Genes & development.

[23]  E. Coen,et al.  The war of the whorls: genetic interactions controlling flower development , 1991, Nature.