Effect of protein–protein interactions on protein aggregation kinetics

The mutual diffusion coefficient Dm of dilute lysozyme solutions is measured by the dynamic light scattering technique. The relation between the osmotic second virial coefficient B22 and the protein mutual diffusion coefficient Dm of dilute solutions is examined experimentally and theoretically. The role of B22 in relation with Dm in protein aggregation kinetics was analyzed based on the effect of the average protein pairwise interactions on the protein aggregation kinetics. The empirical “crystallization window” proposed by George and Wilson [Acta Crystallogr., Sect. D: Biol. Crystallogr. 50, 361 (1994)] can be interpreted from the point view of crystallization kink kinetics.

[1]  Terrell L. Hill.  Lectures on Matter and Equilibrium , 1966 .

[2]  S. Yau,et al.  Quasi-planar nucleus structure in apoferritin crystallization , 2000, Nature.

[3]  A. Arabshahi,et al.  [6] Second virial coefficient as predictor in protein crystal growth. , 1997, Methods in enzymology.

[4]  Richard Giegé,et al.  Crystallogenesis of biological macromolecules. Biological, microgravity and other physico-chemical aspects , 1995 .

[5]  Arnaud Ducruix,et al.  Crystallization of Nucleic Acids and Proteins: A practical Approach , 1998 .

[6]  P. Vekilov,et al.  Nucleation and Crystallization of Globular Proteins: What we Know and What is Missing , 1996 .

[7]  W. Brown.  Dynamic light scattering : the method and some applications , 1993 .

[8]  I. Teraoka.  Polymer solutions: An introduction to physical properties , 2002 .

[9]  O. Velev,et al.  Protein interactions in solution characterized by light and neutron scattering: comparison of lysozyme and chymotrypsinogen. , 1998, Biophysical journal.

[10]  Håkan Wennerström,et al.  The Colloidal Domain: Where Physics, Chemistry, Biology and Technology Meet , 1994 .

[11]  Bruno H. Zimm,et al.  Application of the Methods of Molecular Distribution to Solutions of Large Molecules , 1946 .

[12]  Different tools to study interaction potentials in gamma-crystallin solutions: relevance to crystal growth. , 1997, Acta crystallographica. Section D, Biological crystallography.

[13]  S. Yau,et al.  Molecular-level thermodynamic and kinetic parameters for the self-assembly of apoferritin molecules into crystals. , 2000, Journal of molecular biology.

[14]  F. Rosenberger,et al.  Lack of Evidence for Prenucleation Aggregate Formation in Lysozyme Crystal Growth Solutions , 1996 .

[15]  A. Kierzek,et al.  Microscopic model of protein crystal growth. , 2000, Biophysical chemistry.

[16]  Franz Rosenberger,et al.  Liquid-Liquid Phase Separation in Supersaturated Lysozyme Solutions and Associated Precipitate Formation/Crystallization , 1997 .

[17]  W. G. McMillan,et al.  The Statistical Thermodynamics of Multicomponent Systems , 1945 .

[18]  A. Lenhoff,et al.  Molecular origins of osmotic second virial coefficients of proteins. , 1998, Biophysical journal.

[19]  Xiang-yang Liu,et al.  Protein interactions in undersaturated and supersaturated solutions: a study using light and x-ray scattering. , 2003, Biophysical journal.