The influence of a selected desensitizer on marginal microleakage of a class V composite restoration subjected to thermocycles: an in vitro study.

INTRODUCTION Dentin hypersensitivity is a painful clinical condition. The frequency of its occurrence varies from 8 to 57%, depending on tested group and different methods of investigations. Recommended desensitizing agents have different mechanism of action and effectiveness. We are still looking for solutions that will improve their effectiveness and simultaneously allow for wider use of e.g. as a base material, counteracting postoperative hypersensitivity, reducing marginal microleakage. The aim of the study was to assess the effect of a selected desensitizing agent occluding dentin tubules with calcium hydroxyapatite on marginal microleakage formation of a class V composite restorations subjected to thermocycles. MATERIALS AND METHODS In study it was used 40 molars and premolars, which were alternately assigned into two groups. In both groups standardized cavities were prepared. In the study group (study group - SG) before application of bonding agent Teethmate Desensitizer (f. Kuraray, Noritake Dental Inc., Okayama, Japan) was used. In the control group (control group - CG) OptiBond All-in-one (f. Kerr, Bioggio, Switzerland) bonding agent was used and cavities were filled using composite material Gradia Direct (f. GC Europe N.V., Leuven, Belgium). After storage in saline, teeth were subjected to 600 thermocycles, passive dye penetration test was done, teeth were cut in the area of filling, according to its long axis. Under light microscope magnification value of microleakage was measured and marginal microleakage rate (M) was counted. The results of the tests were statistically analyzed using the package STATISTICA 12.0 (StatSoft, USA). RESULTS The average value of M for the SG group was 0.46 (min 0.05, max 0.76, SD 0.226) and for CG was 0.22 (min 0, max 0.74, SD 0.235). The differences between M values were statistically significant (p = 0.0094). CONCLUSION A reduction in the number of retention sites for the bonding system, facilitates the formation of microleakage in the experimental conditions and reduces the degree of adhesion of the composite material to the hard tissues of the tooth.

[1]  M. Shahabi,et al.  Comparison of self-adhering flowable composite microleakage with several types of bonding agent in class V cavity restoration , 2019, Dental research journal.

[2]  T. Scaramucci,et al.  Effect of in-office desensitizers containing calcium and phosphate on dentin permeability and tubule occlusion. , 2019, Journal of dentistry.

[3]  M. Otsuki,et al.  Effect of Calcium-phosphate Desensitizers on Staining Susceptibility of Acid-eroded Enamel. , 2019, Operative dentistry.

[4]  J. Ferracane,et al.  The role of polymerization in adhesive dentistry. , 2019, Dental materials : official publication of the Academy of Dental Materials.

[5]  W. El-Badrawy,et al.  Gingival microleakage of class II bulk-fill composite resin restorations. , 2018, Dental and medical problems.

[6]  M. Tomita,et al.  Effect of two desensitizing agents on dentin permeability in vitro , 2017, Journal of applied oral science : revista FOB.

[7]  F. Tay,et al.  Effects of a Dicalcium and Tetracalcium Phosphate-Based Desensitizer on In Vitro Dentin Permeability , 2016, PloS one.

[8]  T. Okiji,et al.  Dentin tubule occluding ability of dentin desensitizers. , 2015, American journal of dentistry.

[9]  W. Finger,et al.  Randomized controlled clinical trial on the efficacy of dentin desensitizing agents , 2014, Acta odontologica Scandinavica.

[10]  A. Sadr,et al.  Effect of a calcium-phosphate based desensitizer on dentin surface characteristics. , 2013, Dental materials journal.

[11]  T. Takamizawa,et al.  Evaluation of a calcium phosphate desensitizer using an ultrasonic device. , 2013, Dental materials journal.

[12]  A. Sadr,et al.  In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium-phosphate desensitizer. , 2013, Journal of biomedical materials research. Part B, Applied biomaterials.

[13]  K. Bekes,et al.  What is known about the influence of dentine hypersensitivity on oral health-related quality of life? , 2012, Clinical Oral Investigations.

[14]  S. Acharya,et al.  A comparative evaluation of dentinal hypersensitivity and microleakage associated with composite restorations in cavities preconditioned with air abrasion - An ex vivo study , 2012, Contemporary clinical dentistry.

[15]  L. Chow.  Next generation calcium phosphate-based biomaterials. , 2009, Dental materials journal.

[16]  Cynthia P Trajtenberg,et al.  Microleakage of all-ceramic crowns using self-etching resin luting agents. , 2008, Operative dentistry.

[17]  J. Xu,et al.  Spectroscopic investigation of the function of aqueous 2-hydroxyethylmethacrylate/glutaraldehyde solution as a dentin desensitizer. , 2006, European journal of oral sciences.

[18]  R. Orchardson,et al.  Managing dentin hypersensitivity. , 2006, Journal of the American Dental Association.

[19]  S. Takagi,et al.  Reduction in dentin permeability using mildly supersaturated calcium phosphate solutions. , 2004, Archives of oral biology.

[20]  M. Larsen,et al.  Saturation of human saliva with respect to calcium salts. , 2003, Archives of oral biology.

[21]  J. Egea,et al.  Consensus-based recommendations for the diagnosis and management of dentin hypersensitivity. , 2003, Journal.

[22]  M. Addy.  Dentine hypersensitivity: New perspectives on an old problem , 2002 .

[23]  M. Addy.  Tooth wear and sensitivity : clinical advances in restorative dentistry , 2000 .

[24]  K. Ishikawa,et al.  Reaction of calcium phosphate cements with different amounts of tetracalcium phosphate and dicalcium phosphate anhydrous. , 1999, Journal of biomedical materials research.

[25]  G. R. Holland,et al.  Guidelines for the design and conduct of clinical trials on dentine hypersensitivity. , 1997, Journal of clinical periodontology.

[26]  H. Newman,et al.  Prevalence of cervical dentine sensitivity in a population of patients referred to a specialist Periodontology Department. , 1996, Journal of clinical periodontology.

[27]  R. Orchardson,et al.  Clinical features of hypersensitive teeth , 1987, British Dental Journal.

[28]  M. Addy,et al.  Dentine hypersensitivity--a review. Aetiology, symptoms and theories of pain production. , 1983, Journal of Clinical Periodontology.