Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase

The onset of M phase requires the activation of the pp34 protein kinase in all eukaryotes thus far examined. In Schizosaccharomyces pombe, pp34 is phosphorylated on Tyr15, and dephosphorylation of this residue regulates the initiation of mitosis. In this study, it is shown that dephosphorylation of Tyr15 triggered activation of the pp34-cyclin complex from fission yeast, that a human protein-tyrosine phosphatase can catalyze this event both in vitro and in vivo, and that activation of fission yeast pp34 does not require threonine dephosphorylation. The complementary DNA that encoded the tyrosine phosphatase replaced the mitotic activator p80cdc25, closely associating the cdc25(+)-activating pathway with tyrosine dephosphorylation of pp34.

[1]  K. Maundrell.  nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. , 1990, The Journal of biological chemistry.

[2]  N. Tonks,et al.  CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. , 1990, The Journal of biological chemistry.

[3]  Sergio Moreno,et al.  Regulation of mitosis by cyclic accumulation of p80cdc25 mitotic inducer in fission yeast , 1990, Nature.

[4]  P. Nurse.  Universal control mechanism regulating onset of M-phase , 1990, Nature.

[5]  M. Dorée.  Control of M-phase by maturation-promoting factor. , 1990, Current opinion in cell biology.

[6]  D. Beach,et al.  Fission yeast cdc25 is a cell-cycle regulated protein. , 1990, Biochemical and biophysical research communications.

[7]  D. Beach,et al.  Activation of M-phase-specific histone H1 kinase by modification of the phosphorylation of its p34cdc2 and cyclin components. , 1990, Genes & development.

[8]  N. Tonks,et al.  Protein tyrosine dephosphorylation and signal transduction. , 1989, Trends in biochemical sciences.

[9]  A. Murray,et al.  Dominoes and clocks: the union of two views of the cell cycle. , 1989, Science.

[10]  Kathleen L. Gould,et al.  Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis , 1989, Nature.

[11]  T. Hunter.  Protein-tyrosine phosphatases: The other side of the coin , 1989, Cell.

[12]  Sergio Moreno,et al.  Regulation of p34cdc2 protein kinase during mitosis , 1989, Cell.

[13]  D. Beach,et al.  Reversible tyrosine phosphorylation of cdc2: Dephosphorylation accompanies activation during entry into mitosis , 1989, Cell.

[14]  J. Newport,et al.  Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase , 1989, Cell.

[15]  E. Krebs,et al.  cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. Maller,et al.  Dephosphorylation and activation of Xenopusp34cdc2 protein kinase during the cell cycle , 1989, Nature.

[17]  J. Labbé,et al.  Purification of MPF from starfish: Identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation , 1989, Cell.

[18]  Sergio Moreno,et al.  Conservation of mitotic controls in fission and budding yeasts , 1989, Cell.

[19]  B. Sefton,et al.  Acid and base hydrolysis of phosphoproteins bound to immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins. , 1989, Analytical biochemistry.

[20]  D. Beach,et al.  Activation of cdc2 protein kinase during mitosis in human cells: Cell cycle-dependent phosphorylation and subunit rearrangement , 1988, Cell.

[21]  N. Tonks,et al.  Characterization of the major protein-tyrosine-phosphatases of human placenta. , 1988, The Journal of biological chemistry.

[22]  Paul Russell,et al.  Negative regulation of mitosis by wee1 +, a gene encoding a protein kinase homolog , 1987, Cell.

[23]  P. Nurse,et al.  The cell cycle control gene cdc2 + of fission yeast encodes a protein kinase potentially regulated by phosphorylation , 1986, Cell.

[24]  Paul Russell,et al.  cdc25 + functions as an inducer in the mitotic control of fission yeast , 1986, Cell.

[25]  T. Hunter,et al.  Detection and quantification of phosphotyrosine in proteins. , 1983, Methods in enzymology.

[26]  P. Nurse,et al.  Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast , 1981, Nature.

[27]  P. Fantes.  Isolation of cell size mutants of a fission yeast by a new selective method: characterization of mutants and implications for division control mechanisms , 1981, Journal of bacteriology.

[28]  P. Thuriaux,et al.  Regulatory genes controlling mitosis in the fission yeast Schizosaccharomyces pombe. , 1980, Genetics.

[29]  Paul Nurse,et al.  Genetic control of cell size at cell division in yeast , 1975, Nature.