Applications of antibody array platforms.

Antibody arrays are valuable for the parallel analysis of multiple proteins in small sample volumes. The earliest and most widely used application of antibody arrays has been to measure multiple protein abundances, using sandwich assays and label-based assays, for biomarker discovery and biological studies. Modifications to these assays have led to studies profiling specific protein post-translational modifications. Additional novel uses include profiling enzyme activities and protein cell-surface expression. Finally, array-based antibody platforms are being used to assist the development and characterization of antibodies. Continued progress in the technology will surely lead to extensions of these applications and the development of new ways of using the methods.

[1]  S. Kingsmore Multiplexed protein measurement: technologies and applications of protein and antibody arrays , 2006, Nature Reviews Drug Discovery.

[2]  P. Predki,et al.  Functional protein microarrays: just how functional are they? , 2005, Current opinion in biotechnology.

[3]  S. Kingsmore,et al.  Cerebral palsy is characterized by protein mediators in cord serum , 2004, Annals of neurology.

[4]  B. Cravatt,et al.  Microarray platform for profiling enzyme activities in complex proteomes. , 2004, Journal of the American Chemical Society.

[5]  Barry Schweitzer,et al.  Microarrays to characterize protein interactions on a whole‐proteome scale , 2003, Proteomics.

[6]  Jonathan S. Reichner,et al.  Antibodies Immobilized as Arrays to Profile Protein Post-translational Modifications in Mammalian Cells* , 2004, Molecular & Cellular Proteomics.

[7]  S. Schreiber,et al.  Printing proteins as microarrays for high-throughput function determination. , 2000, Science.

[8]  A. Chinnaiyan,et al.  Using protein microarrays to study cancer. , 2002, BioTechniques.

[9]  H. Pfister,et al.  Patterns of protein expression in infectious meningitis: A cerebrospinal fluid protein array analysis , 2005, Journal of Neuroimmunology.

[10]  P. Sorger,et al.  Profiling receptor tyrosine kinase activation by using Ab microarrays , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[11]  T. Barrette,et al.  Profiling of cancer cells using protein microarrays: discovery of novel radiation-regulated proteins. , 2001, Cancer research.

[12]  Ziding Feng,et al.  Antibody microarray profiling reveals individual and combined serum proteins associated with pancreatic cancer. , 2005, Cancer research.

[13]  P. Angenendt Progress in protein and antibody microarray technology. , 2005, Drug discovery today.

[14]  S. Kingsmore,et al.  Immunoassays with rolling circle DNA amplification: a versatile platform for ultrasensitive antigen detection. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[15]  David E. Misek,et al.  Distinctive serum protein profiles involving abundant proteins in lung cancer patients based upon antibody microarray analysis , 2005, BMC Cancer.

[16]  Soo-Ik Chang,et al.  Profiling of differential protein expression in angiogenin‐induced HUVECs using antibody‐arrayed ProteoChip , 2006, Proteomics.

[17]  E. B. Butler,et al.  Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers , 2003, Proteomics.

[18]  Hongyu Zhao,et al.  Serum protein markers for early detection of ovarian cancer. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[19]  J. Terrett,et al.  Competitive Assay Formats for High-Throughput Affinity Arrays , 2003, Journal of biomolecular screening.

[20]  David Krumholz,et al.  Membrane array characterization of 80 chemokines, cytokines, and growth factors in open- and closed-eye tears: angiogenin and other defense system constituents. , 2005, Investigative ophthalmology & visual science.

[21]  LT Perlee,et al.  Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics , 2004, Proteome Science.

[22]  Paul Lizardi,et al.  Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements , 2004, Genome Biology.

[23]  Ruo-Pan Huang,et al.  Profiling of cytokine expression by biotin‐labeled‐based protein arrays , 2003, Proteomics.

[24]  S. Kingsmore,et al.  Multiplexed protein profiling on microarrays by rolling-circle amplification , 2002, Nature Biotechnology.

[25]  S. Fields,et al.  Protein analysis on a proteomic scale , 2003, Nature.

[26]  Paul Tempst,et al.  A Prototype Antibody Microarray Platform to Monitor Changes in Protein Tyrosine Phosphorylation* , 2004, Molecular & Cellular Proteomics.

[27]  M. Snyder,et al.  Protein chip technology. , 2003, Current opinion in chemical biology.

[28]  Wei Huang,et al.  Serum proteomic signature for cystic fibrosis using an antibody microarray platform. , 2006, Molecular genetics and metabolism.

[29]  R. Christopherson,et al.  Multiplex detection of surface molecules on colorectal cancers , 2006, Proteomics.

[30]  R. Christopherson,et al.  Identification of repertoires of surface antigens on leukemias using an antibody microarray , 2003, Proteomics.

[31]  S. Kingsmore,et al.  Protein Microarray Analysis of Disease Activity in Pediatric Inflammatory Bowel Disease Demonstrates Elevated Serum PLGF, IL-7, TGF-β1, and IL-12p40 Levels in Crohn's Disease and Ulcerative Colitis Patients in Remission versus Active Disease , 2005, The American Journal of Gastroenterology.

[32]  Brian B Haab,et al.  Antibody Arrays in Cancer Research , 2005, Molecular & Cellular Proteomics.

[33]  L. Liotta,et al.  Proteomic profiling of the cancer microenvironment by antibody arrays , 2001, Proteomics.

[34]  David P. Kreil,et al.  Conservation of unique cell-surface CD antigen mosaics in HIV-1-infected individuals. , 2005, Blood.

[35]  R. Christopherson,et al.  Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. , 2001, Cancer research.

[36]  B. Haab Methods and applications of antibody microarrays in cancer research , 2003, Proteomics.

[37]  P. Brown,et al.  Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions , 2001, Genome Biology.

[38]  R. Huang,et al.  Simultaneous detection of multiple cytokines from conditioned media and patient's sera by an antibody-based protein array system. , 2001, Analytical biochemistry.

[39]  Ziding Feng,et al.  Optimized Normalization for Antibody Microarrays and Application to Serum-Protein Profiling*S , 2005, Molecular & Cellular Proteomics.