A rapid measurement for cotton breeders of maturity and fineness from developing and mature fibers

The Cottonscope simultaneously measures a cotton fiber’s maturity and fineness using a small amount of fiber sample. A program of testing was devised to establish the potential and capabilities of the Cottonscope to rapidly and accurately measure maturity and fineness of small quantities of near-isogenic cotton lines (NILs), and to examine the use of the Cottonscope maturity distributions for breeder applications. Cottonscope measurements were performed on mature and immature fibers of varying days post-anthesis (DPA) from two pairs of NILs (MD 52ne versus MD 90ne; TM-1 versus the low maturity im). The patterns of cotton maturity and fineness during cotton fiber development of each NIL measured by the Cottonscope were compared to those measured by more conventional methods (e.g. the Advanced Fiber Information System (AFIS), an older and currently more widely used method). The Cottonscope maturity and fineness results were much more responsive to increasing DPA than the AFIS results, and the patterns of Cottonscope maturity values were consistent with those of the Fibronaire micronaire. Comparisons of the Cottonscope maturity distributions among the NILs demonstrated that the maturity distribution for im was very different and exhibited much lower maturity values compared to the distributions of other lines. The results demonstrated that the Cottonscope is an efficient instrument for cotton breeders to monitor fiber maturity and fineness of developing and mature cotton fibers.

[1]  Molecular markers associated with the immature fiber (im) gene affecting the degree of fiber cell wall thickening in cotton (Gossypiumhirsutum L.) , 2012, Theoretical and Applied Genetics.

[2]  Devron Thibodeaux,et al.  Rapid measurement of cotton fiber maturity and fineness by image analysis microscopy using the Cottonscope® , 2012 .

[3]  Hh Jr Ramey,et al.  Estimating Quality Components of Natural Fibers by Near-Infrared Reflectance1 , 1982 .

[4]  Z. Chen,et al.  Near-isogenic cotton germplasm lines that differ in fiber-bundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling , 2010, Theoretical and Applied Genetics.

[5]  W. Meredith Registration of MD 52ne High Fiber Quality Cotton Germplasm and Recurrent Parent MD 90ne , 2005 .

[6]  D. P. Thibodeaux,et al.  COTTONSCOPE – A NEW INSTRUMENT FOR MATURITY AND FINENESS MEASUREMENTS : b ) EXPERIMENTAL RESULTS AND EXPERIENCES , 2015 .

[7]  Stuart Gordon,et al.  Measuring the Maturity of Developing Cotton Fibers using an Automated Polarized Light Microscopy Technique , 2010 .

[8]  R. Kohel,et al.  Texas Marker-1. Description of a Genetic Standard for Gossypium hirsutum L. 1 , 1970 .

[9]  Eric Hequet,et al.  Creation of a Set of Reference Material for Cotton Fiber Maturity Measurements , 2006 .

[10]  Svend A. Larsen,et al.  Estimating Quality Components of Natural Fibers by Near-Infrared Reflectance1 , 1982 .

[11]  Joseph G. Montalvo,et al.  Analysis of Cotton , 2015 .

[12]  G. R. S. Naylor,et al.  COTTONSCOPE: A NEW INSTRUMENT FOR MATURITY AND FINENESS , 2015 .

[13]  J. P. Evans,et al.  A quick embedding method for light microscopy and image analysis of cotton fibers. , 1995, Biotechnic & Histochemistry.

[14]  F. T. Peirce D.Sc.,et al.  13—THE FINENESS AND MATURITY OF COTTON , 1939 .

[15]  R. Kohel,et al.  Immature fiber mutant of Upland cotton. , 1990 .