Animated Pseudocolor Activity Maps (Pam’s): Scientific Visualization of Brain Electrical Activity

Major advances in neuroscience have often followed directly from the application of new and more powerful methodological approaches to the study of brain structure and function (Clarke & Jacyna, 1987). Within the last decade a new technique has been developed that allows both brain structure and function to be studied in a closely integrated and highly complimentary fashion. This technique is multiple-site optical recording of membrane potential, or more simply, optical recording. Optical recording is based upon the ability of certain vital dyes (potentiometric probes) to optically signal changes in intracellular membrane potential. By viewing brain tissue stained with a voltage-sensitive dye with a suitable light detector system, changes in neuronal activity can be monitored simultaneously from a 100 or more contiguous anatomical regions (cf. Grinvald et al., 1988).

[1]  S. Carlill.  Olfaction and Taste II , 1968 .

[2]  D. Senseman,et al.  Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components , 1983, Nature.

[3]  Elizabeth C. Crosly,et al.  The Brain of the Tiger Salamander. , 1949 .

[4]  D. Senseman,et al.  Odor-elicited activity monitored simultaneously from 124 regions of the salamander olfactory bulb using a voltage-sensitive dye , 1987, Brain Research.

[5]  Adrian Ed,et al.  Sensory discrimination with some recent evidence from the olfactory organ. , 1950 .

[6]  L. Cohen,et al.  Optical monitoring of activity from many areas of the in vitro and in vivo salamander olfactory bulb: a new method for studying functional organization in the vertebrate central nervous system , 1983, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[7]  Paul De Weer,et al.  Optical methods in cell physiology , 1986 .

[8]  J. Kauer.  Real-time imaging of evoked activity in local circuits of the salamander olfactory bulb , 1988, Nature.

[9]  D. G. Moulton.  SPATIO-TEMPORAL PATTERNING OF RESPONSE IN THE OLFACTORY SYSTEM , 1967 .

[10]  R. Gesteland.  Neural Coding in Olfactory Receptor Cells , 1971 .

[11]  André Duchamp,et al.  QUALITATIVE AND QUANTITATIVE DISCRIMINATION IN THE FROG OLFACTORY RECEPTORS: ANALYSIS FROM ELECTROPHYSIOLOGICAL DATA , 1974, Annals of the New York Academy of Sciences.

[12]  L. S. Jacyna,et al.  Nineteenth-Century Origins of Neuroscientific Concepts , 1987 .

[13]  D. Senseman,et al.  MSORTV imaging of electrotonic conduction in a syncitium: optical recording of polarization spread in a simple salivary gland. , 1987, The Journal of experimental zoology.

[14]  T. Getchell.  Unitary Responses in Frog Olfactory Epithelium to Sterically Related Molecules at Low Concentrations , 1974, The Journal of general physiology.

[15]  G. Shepherd,et al.  Responses of olfactory receptor cells to step pulses of odour at different concentrations in the salamander. , 1978, The Journal of physiology.

[16]  Anders Liljeborg.  Digital Position Encoding Of Galvanometer Scanner In A Laser Microscope , 1988 .

[17]  D. G. Moulton,et al.  Spatial patterning of response to odors in the peripheral olfactory system. , 1976, Physiological reviews.

[18]  D. G. Moulton,et al.  ELECTROPHYSIOLOGY OF THE OLFACTORY SYSTEM * , 1964, Annals of the New York Academy of Sciences.

[19]  E. D. Adrian,et al.  I. - Olfactory discrimination , 1949 .

[20]  David M. Senseman,et al.  High-speed imaging of electrical activity: Watching the brain think on MTV/2 , 1988, Neural Networks.

[21]  D. G. Moulton,et al.  Structure and function in the peripheral olfactory system. , 1967, Physiological reviews.

[22]  R. Frostig,et al.  Optical imaging of neuronal activity. , 1988, Physiological reviews.

[23]  M. M. Mozell,et al.  Quantitative stimulation of frog olfactory receptors. , 1969, Journal of Neurophysiology.