An Impedance Sensing Platform for Monitoring Heterogeneous Connectivity and Diagnostics in Lab-on-a-Chip Systems

Reliable hardware connectivity is vital in heterogeneous integrated systems. For example, in digital microfluidics lab-on-a-chip systems, there are hundreds of physical connections required between a microelectromechanical fabricated device and the driving system that can be remotely located on a printed circuit board. Unfortunately, the connection reliability cannot be checked or monitored by vision-based detection methods that are commonly used in the semiconductor industry. Therefore, a sensing platform that can be seamlessly integrated into existing digital microfluidics systems and provide real-time monitoring of multiconnectivity is highly desired. Here, we report an impedance sensing platform that can provide fast detection of a single physical connection in timescales of milliseconds. Once connectivity is established, the same setup can be used to determine the droplet location. The sensing system can be scaled up to support multiple channels or applied to other heterogeneously integrated systems that require real-time monitoring and diagnostics of multiconnectivity systems.

[1]  Jun Zeng,et al.  Principles of droplet electrohydrodynamics for lab-on-a-chip. , 2004, Lab on a chip.

[2]  Aaron Wheeler,et al.  Putting Electrowetting to Work , 2008, Science.

[3]  D. Hasko,et al.  Influence of polarization on contact angle saturation during electrowetting , 2016 .

[4]  Ehsan Samiei,et al.  A review of digital microfluidics as portable platforms for lab-on a-chip applications. , 2016, Lab on a chip.

[5]  Noel S Ha,et al.  Ionic-surfactant-mediated electro-dewetting for digital microfluidics , 2019, Nature.

[6]  A. Wheeler,et al.  The Digital Revolution: A New Paradigm for Microfluidics , 2009 .

[7]  Bernhard Weigl,et al.  Towards non- and minimally instrumented, microfluidics-based diagnostic devices. , 2008, Lab on a chip.

[8]  A. Wheeler,et al.  Digital microfluidics and nuclear magnetic resonance spectroscopy for in situ diffusion measurements and reaction monitoring. , 2019, Lab on a chip.

[9]  G.E. Moore,et al.  Cramming More Components Onto Integrated Circuits , 1998, Proceedings of the IEEE.

[10]  F. E. Critchfield,et al.  Dielectric Constant and Refractive Index from 20 to 35° and Density at 25° for the System Tetrahydrofuran—Water1 , 1953 .

[11]  J. Baret,et al.  Electrowetting: from basics to applications , 2005 .

[12]  Eric P. Y. Chiou,et al.  EWOD-driven droplet microfluidic device integrated with optoelectronic tweezers as an automated platform for cellular isolation and analysis. , 2009, Lab on a chip.

[13]  R. Fair,et al.  Electrowetting-based actuation of droplets for integrated microfluidics. , 2002, Lab on a chip.

[14]  Michael D M Dryden,et al.  A digital microfluidic system for serological immunoassays in remote settings , 2018, Science Translational Medicine.

[15]  Aaron R Wheeler,et al.  A microfluidic platform for complete mammalian cell culture. , 2010, Lab on a chip.

[16]  R. Fair,et al.  Electrowetting-based actuation of liquid droplets for microfluidic applications , 2000 .

[17]  H. Morgan,et al.  Programmable large area digital microfluidic array with integrated droplet sensing for bioassays. , 2012, Lab on a chip.

[18]  S. Kalsi,et al.  Rapid and sensitive detection of antibiotic resistance on a programmable digital microfluidic platform. , 2015, Lab on a chip.

[19]  A. A. Maryott,et al.  Dielectric Constant of Water from 0 0 to 100 0 C , 2011 .

[20]  Teodor Veres,et al.  Integration and detection of biochemical assays in digital microfluidic LOC devices. , 2010, Lab on a chip.

[21]  Matthew T. Cole,et al.  Flexible Electronics: The Next Ubiquitous Platform , 2012, Proceedings of the IEEE.

[22]  R. Murray,et al.  Basics or Applications , 1998 .

[23]  Mirela Alistar,et al.  OpenDrop: An Integrated Do-It-Yourself Platform for Personal Use of Biochips , 2017, Bioengineering.

[24]  A. Wheeler,et al.  Digital microfluidics for cell-based assays. , 2008, Lab on a chip.

[25]  Allen R. Hefner,et al.  An analytical model for the steady-state and transient characteristics of the power insulated-gate bipolar transistor , 1988 .

[26]  A. Wheeler,et al.  DropBot: An open-source digital microfluidic control system with precise control of electrostatic driving force and instantaneous drop velocity measurement , 2013 .

[27]  A. A. Maryott,et al.  Dielectric constant of water from 0 to 100 C , 1956 .