Continuous sorting and separation of microparticles by size using AC dielectrophoresis in a PDMS microfluidic device with 3‐D conducting PDMS composite electrodes

Soft lithography technology allows for the development of numerous PDMS‐based microfluidic devices for manipulation of particles and cells. However, integrating metallic electrodes with PDMS‐based channel structures is challenging due to weak adhesion between metal and PDMS. To overcome this issue, we develop a new PDMS‐based microfluidic device for continuous sorting and separation of microparticles by size using AC dielectrophoresis (DEP) with 3‐D conducting PDMS composites as sidewall electrodes. The composites are synthesized by mixing silver powders with PDMS gel and such composite electrodes can easily be integrated with the PDMS microchannels. Furthermore, the sidewall electrodes also allow DEP forces to distribute three dimensionally, thus enhancing DEP effects in the entire region of channels. The capability of such PDMS‐based microfluidic device is demonstrated for continuously sorting and separating 10 and 15 μm particles, and also for separating 5 from 10 μm particles. Together with experimental results, analysis of particle's trajectory based on Lagrangian approach provides insights into how microparticles transport under the effects of hydrodynamic and DEP forces in the present PDMS‐based microfluidic device.

[1]  S. Kalams,et al.  DC-Dielectrophoretic separation of biological cells by size , 2008, Biomedical microdevices.

[2]  J. Kutter,et al.  Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter. , 2003, Lab on a chip.

[3]  J. Voldman,et al.  Dielectrophoretic registration of living cells to a microelectrode array. , 2004, Biosensors & bioelectronics.

[4]  Yuejun Kang,et al.  Continuous particle separation with localized AC-dielectrophoresis using embedded electrodes and an insulating hurdle , 2009 .

[5]  N. Aubry,et al.  Dielectrophoresis induced clustering regimes of viable yeast cells , 2005, Electrophoresis.

[6]  Lisen Wang,et al.  Side-Wall Vertical Electrodes for Lateral Field Microfluidic Applications , 2007, Journal of Microelectromechanical Systems.

[7]  I. T. Young,et al.  Size-dependent trajectories of DNA macromolecules due to insulative dielectrophoresis in submicrometer-deep fluidic channels. , 2008, Biomicrofluidics.

[8]  Y. Lam,et al.  Assessment of Joule heating and its effects on electroosmotic flow and electrophoretic transport of solutes in microfluidic channels , 2006, Electrophoresis.

[9]  Jonghyun Oh,et al.  Comprehensive analysis of particle motion under non-uniform AC electric fields in a microchannel. , 2009, Lab on a chip.

[10]  Marc J Madou,et al.  3‐D electrode designs for flow‐through dielectrophoretic systems , 2005, Electrophoresis.

[11]  Y. Lam,et al.  Dielectrophoretic manipulation of particles in a modified microfluidic H filter with multi-insulating blocks. , 2008, Biomicrofluidics.

[12]  P. Sheng,et al.  Characterizing and Patterning of PDMS‐Based Conducting Composites , 2007 .

[13]  Andreas Radbruch,et al.  High gradient magnetic cell separation with MACS. , 1990, Cytometry.

[14]  Hywel Morgan,et al.  AC ELECTROKINETICS: COLLOIDS AND NANOPARTICLES. , 2002 .

[15]  E. Cummings,et al.  Insulator‐based dielectrophoresis for the selective concentration and separation of live bacteria in water , 2004, Electrophoresis.

[16]  Paschalis Alexandridis,et al.  Using nonuniform electric fields to accelerate the transport of viruses to surfaces from media of physiological ionic strength. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[17]  Joel Voldman,et al.  Dielectrophoretic traps for single-particle patterning. , 2005, Biophysical journal.

[18]  C. H. Kua,et al.  Dynamic cell fractionation and transportation using moving dielectrophoresis. , 2007, Analytical chemistry.

[19]  Byungkyu Kim,et al.  An efficient cell separation system using 3D-asymmetric microelectrodes. , 2005, Lab on a chip.

[20]  Yuejun Kang,et al.  Continuous particle separation by size via AC‐dielectrophoresis using a lab‐on‐a‐chip device with 3‐D electrodes , 2009, Electrophoresis.

[21]  Conrad D. James,et al.  Continuous-mode dielectrophoretic gating for highly efficient separation of analytes in surface micromachined microfluidic devices , 2008 .

[22]  Zachary Gagnon,et al.  Dielectrophoretic discrimination of bovine red blood cell starvation age by buffer selection and membrane cross-linking. , 2007, Biomicrofluidics.

[23]  Abraham P Lee,et al.  Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow‐through separation of beads and cells , 2009, Electrophoresis.

[24]  Thomas B. Jones,et al.  Electromechanics of Particles , 1995 .

[25]  Ciprian Iliescu,et al.  Fabrication of a dielectrophoretic chip with 3D silicon electrodes , 2005 .

[26]  Chun Yang,et al.  Analysis of electrokinetic transport of a spherical particle in a microchannel , 2007, Electrophoresis.

[27]  M. Stelzle,et al.  Microdevices for manipulation and accumulation of micro‐ and nanoparticles by dielectrophoresis , 2003, Electrophoresis.

[28]  S G Shirley,et al.  Dielectrophoretic sorting of particles and cells in a microsystem. , 1998, Analytical chemistry.

[29]  H. Morgan,et al.  Electrohydrodynamics and dielectrophoresis in microsystems: scaling laws , 2003 .

[30]  Noo Li Jeon,et al.  Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry. , 2007, Lab on a chip.

[31]  Martin Stelzle,et al.  Accumulation and trapping of hepatitis A virus particles by electrohydrodynamic flow and dielectrophoresis , 2006, Electrophoresis.

[32]  Junjie Zhu,et al.  Dielectrophoretic focusing of particles in a microchannel constriction using DC‐biased AC flectric fields , 2009, Electrophoresis.