Non-Destructive Estimation Pigment Content Ripening Quality and Damage in Apple Fruit with Spectral Reflectance in the Visible Range

Modern non-destructive optical-reflectance-based techniques for estimation of pigment (chlorophyll, carotenoid, anthocyanin, and flavonol) contents, the rate of onand off-tree ripening as well as for detection of common physiological disorders, such as sunscald, superficial scald, and water core, and other damages to apple fruit are reviewed with an emphasis on the methods developed by the authors. The basic spectral features of fruit reflectance in the visible and near infra-red are briefly considered together with their implications for the development of algorithms for non-destructive pigment content assessment. The use of reflectance spectroscopy for estimating chlorophyll and carotenoid content as well as carotenoid/chlorophyll ratio during fruit ripening is demonstrated. The algorithms developed for fruit peel pigment analysis and for estimation of ripeness are presented with consideration of the limits of their applicability. Special attention is paid to adaptation of apple fruit to strong sunlight at preharvest stage and its consequences for postharvest fruit quality. _____________________________________________________________________________________________________________

[1]  K. Norris,et al.  The spectrophotometry of dense light-scattering material. , 1960, Archives of biochemistry and biophysics.

[2]  R. Sharples A Note on the Occurrence of Watercore Breakdown in Apples during 1966 , 1967 .

[3]  P. Sharpe,et al.  Genetics and physiology of sunscald of fruits , 1971 .

[4]  M. Ceponis,et al.  Market diseases of apples, pears, and quinces. , 1971 .

[5]  M. Knee Anthocyanin, Carotenoid, and Chlorophyll Changes in the Peel of Cox’s Orange Pippin Apples during Ripening on and off the Tree , 1972 .

[6]  K. Norris,et al.  Co-pigmentation of anthocyanins in plant tissues and its effect on color , 1972 .

[7]  S. Edward Law,et al.  Kubelka-Munk Light-Scattering Coefficients of Model Particulate Systems , 1973 .

[8]  W. F. McClure,et al.  A Spectrophotometric Technique for Studying the Browning Reaction in Tobacco , 1975 .

[9]  T. Goodwin Chemistry and biochemistry of plant pigments , 1976 .

[10]  E. Simon THE SYMPTOMS OF CALCIUM DEFICIENCY IN PLANTS , 1978 .

[11]  T. Shiga,et al.  Application of Light Reflectance Properties of Satsuma Oranges to Automatic Grading in the Packinghouse Line (I) , 1978 .

[12]  Application of Light Reflectance Properties of Satsuma Oranges to Automatic Grading in the Packinghouse Line Relationship between Grading Index and Spectral Reflectance , 1981 .

[13]  S. Duke,et al.  Function of polyphenol oxidase in higher plants , 1984 .

[14]  J. Gross Pigments in fruits , 1987 .

[15]  M. Knee Carotenol esters in developing apple fruits , 1988 .

[16]  M. Saure External control of anthocyanin formation in apple , 1990 .

[17]  Light Reflectance Properties of Defects of Satsuma Mandarin , 1990 .

[18]  R. Ben-arie,et al.  Darkening of sunscald on apples in storage is a non-enzymatic and non-oxidative process , 1991 .

[19]  M. Blanke,et al.  Light transmission into apple fruit and leaves , 1992 .

[20]  Evaluation of Change in Quality of Ripening Bananas Using Light Reflectance Technique , 1992 .

[21]  Thomas C. Vogelmann,et al.  Plant Tissue Optics , 1993 .

[22]  E. Oliveros,et al.  Antioxidant activity of flavonoids: Efficiency of singlet oxygen (1Δg) quenching , 1993 .

[23]  Michael C. Taylor,et al.  Skin Color in Apples—Influence of Copigmentation and Plastid Pigments on Shade and Darkness of Red Color in Five Genotypes , 1994 .

[24]  John R. L. Walker,et al.  Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar , 1994 .

[25]  W. Bramlage,et al.  Separating the Effects of Low Temperature, Ripening, and Light on Loss of Scald Susceptibility in Apples before Harvest , 1994 .

[26]  William,et al.  A . High Temperature B . Low Temperature SERIAL REVIEW CAROTENOID , 2004 .

[27]  A. Gitelson,et al.  Non-destructive determination of chlorophyll content of leaves of a green and an aurea mutant of tobacco by reflectance measurements , 1996 .

[28]  A. Gitelson,et al.  Detection of Red Edge Position and Chlorophyll Content by Reflectance Measurements Near 700 nm , 1996 .

[29]  A. Gitelson,et al.  Signature Analysis of Leaf Reflectance Spectra: Algorithm Development for Remote Sensing of Chlorophyll , 1996 .

[30]  W. Jongen,et al.  Flavonoids as bioactive components in apple products. , 1997, Cancer Letters.

[31]  Janet F. Bornman,et al.  Plants and UV-B: Ultraviolet radiation as a stress factor and the role of protective pigments , 1997 .

[32]  Anatoly A. Gitelson,et al.  Reflectance spectra of leaves and fruits during their development and senescence and under stress , 1997 .

[33]  C. Rice-Evans,et al.  Antioxidant properties of phenolic compounds , 1997 .

[34]  W. Bramlage,et al.  Effects of temperature, light, and rainfall on superficial scald susceptibility in apples , 1997 .

[35]  A Escarpa,et al.  High-performance liquid chromatography with diode-array detection for the determination of phenolic compounds in peel and pulp from different apple varieties. , 1998, Journal of chromatography. A.

[36]  Josep Peñuelas,et al.  Visible and near-infrared reflectance techniques for diagnosing plant physiological status , 1998 .

[37]  K. R. Markham,et al.  Tautomerism of flavonol glucosides: relevance to plant UV protection and flower colour , 1998 .

[38]  A. Gitelson,et al.  Light-induced pigment degradation in leaves and ripening fruits studied in situ with reflectance spectroscopy , 1998 .

[39]  B. Nicolai,et al.  NON-DESTRUCTIVE MEASUREMENT OF ACIDITY, SOLUBLE SOLIDS, AND FIRMNESS OF JONAGOLD APPLES USING NIR-SPECTROSCOPY , 1998 .

[40]  Linda Chalker-Scott,et al.  Environmental Significance of Anthocyanins in Plant Stress Responses , 1999 .

[41]  P. Reay The role of low temperatures in the development of the red blush on apple fruit (`Granny Smith') , 1999 .

[42]  A. Vainstein,et al.  Carotenoid sequestration in plants: the role of carotenoid-associated proteins. , 1999, Trends in plant science.

[43]  A. Gitelson,et al.  Non‐destructive optical detection of pigment changes during leaf senescence and fruit ripening , 1999 .

[44]  J. DeEll,et al.  Using the Streif Index as a Final Harvest Window for Controlled-atmosphere Storage of Apples , 1999 .

[45]  Allan B. Woolf,et al.  Preharvest factors affecting physiological disorders of fruit , 1999 .

[46]  M. Merzlyak,et al.  Light-stress-induced pigment changes and evidence for anthocyanin photoprotection in apples. , 2000, Journal of photochemistry and photobiology. B, Biology.

[47]  R. Butler,et al.  Induction of flavonoids and phenolic acids in apple by UV-B and temperature , 2000 .

[48]  M. Awad,et al.  Flavonoid and chlorogenic acid levels in apple fruit : characterisation of variation , 2000 .

[49]  Anatoly A. Gitelson,et al.  Reflectance Spectral Features and Detection of Superficial Scald–induced Browning in Storing Apple Fruit , 2001 .

[50]  Alessandro Torricelli,et al.  Time-Resolved Reflectance Spectroscopy Applied to the Nondestructive Monitoring of the Internal Optical Properties in Apples , 2001 .

[51]  J. Lancaster,et al.  Accumulation of anthocyanins and quercetin glycosides in ‘Gala’ and ‘Royal Gala’ apple fruit skin with UV-B–Visible irradiation: modifying effects of fruit maturity, fruit side, and temperature , 2001 .

[52]  A. Peirs,et al.  Prediction of the optimal picking date of different apple cultivars by means of VIS/NIR-spectroscopy , 2001 .

[53]  A. Gitelson,et al.  Optical Properties and Nondestructive Estimation of Anthocyanin Content in Plant Leaves¶ , 2001, Photochemistry and photobiology.

[54]  V. Mcglone,et al.  Vis/NIR estimation at harvest of pre- and post-storage quality indices for 'Royal Gala' apple , 2002 .

[55]  Ingo Truppel,et al.  An approach to non-destructive apple fruit chlorophyll determination , 2002 .

[56]  M. Merzlyak,et al.  Photostability of pigments in ripening apple fruit: a possible photoprotective role of carotenoids during plant senescence , 2002 .

[57]  A. Gitelson,et al.  Assessing Carotenoid Content in Plant Leaves with Reflectance Spectroscopy¶ , 2002, Photochemistry and photobiology.

[58]  A. Gitelson,et al.  Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit , 2003 .

[59]  J. Dole,et al.  1-Methylcyclopropene: a review , 2003 .

[60]  M. Merzlyak,et al.  Optical properties and contribution of cuticle to UV protection in plants: experiments with apple fruit , 2003, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[61]  A. Solovchenko,et al.  Significance of skin flavonoids for UV-B-protection in apple fruits. , 2003, Journal of experimental botany.

[62]  Yuri A. Gritz,et al.  Relationships between leaf chlorophyll content and spectral reflectance and algorithms for non-destructive chlorophyll assessment in higher plant leaves. , 2003, Journal of plant physiology.

[63]  F. Ma,et al.  The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate–glutathione pathway than the shaded peel , 2003 .

[64]  M. Zude Comparison of indices and multivariate models to non-destructively predict the fruit chlorophyll by means of visible spectrometry in apple fruit , 2003 .

[65]  R. Acquaviva,et al.  Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors , 2004, Cell Biology and Toxicology.

[66]  K. Razi Naqvi,et al.  Does a leaf absorb radiation in the near infrared (780–900 nm) region? A new approach to quantifying optical reflection, absorption and transmission of leaves , 2004, Photosynthesis Research.

[67]  M. Merzlyak,et al.  A Spectrophotometric Analysis of Pigments in Apples , 2001, Russian Journal of Plant Physiology.

[68]  Rui Hai Liu,et al.  Apple phytochemicals and their health benefits , 2004, Nutrition journal.

[69]  M. Tevini,et al.  Composition and function of plastoglobuli , 2004, Planta.

[70]  Roberto Oberti,et al.  OPTICAL TECHNIQUES TO ESTIMATE THE RIPENESS OF RED-PIGMENTED FRUITS , 2004 .

[71]  F. Ma,et al.  Exposure of the shaded side of apple fruit to full sun leads to up-regulation of both the xanthophyll cycle and the ascorbate–glutathione cycle , 2004 .

[72]  C. Varga,et al.  A REVIEW OF THE METEOROLOGICAL CAUSES OF SUNBURN INJURY ON THE SURFACE OF APPLE FRUIT (Malus domestica BORKH) , 2004 .

[73]  S. I. Pogosyan,et al.  Application of Reflectance Spectroscopy for Analysis of Higher Plant Pigments , 2003, Russian Journal of Plant Physiology.

[74]  B. Nicolai,et al.  Non-destructive assessment of pigments in apple fruit and carrot by laser-induced fluorescence spectroscopy (LIFS) measured at different time-gate positions , 2005 .

[75]  M. Merzlyak,et al.  Relationships between chlorophyll and carotenoid pigments during on- and off-tree ripening of apple fruit as revealed non-destructively with reflectance spectroscopy , 2005 .

[76]  A. Gitelson,et al.  Apple flavonols during fruit adaptation to solar radiation: spectral features and technique for non-destructive assessment. , 2005, Journal of plant physiology.

[77]  M. Merzlyak Modeling pigment contributions to spectral reflection of apple fruit. , 2006, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[78]  Grethe Iren A. Borge,et al.  Chlorophyll fluorescence as a tool for non-destructive estimation of anthocyanins and total flavonoids in apples , 2006 .

[79]  A. Gitelson,et al.  Three‐band model for noninvasive estimation of chlorophyll, carotenoids, and anthocyanin contents in higher plant leaves , 2006 .

[80]  J. Roger,et al.  Non-destructive tests on the prediction of apple fruit flesh firmness and soluble solids content on tree and in shelf life , 2006 .

[81]  M. Noferini,et al.  USING NIRS TO DETERMINE INTRINSIC FRUIT QUALITY AND HARVEST DATE , 2006 .

[82]  M. Merzlyak,et al.  Elevated sunlight promotes ripening-associated pigment changes in apple fruit , 2006 .

[83]  Pol Coppin,et al.  Detection of biotic stress (Venturia inaequalis) in apple trees using hyperspectral data: Non-parametric statistical approaches and physiological implications , 2007 .

[84]  Non-Destructive Evaluation of Apple Fruit Maturity on the Tree , 2007 .

[85]  Michael Knee,et al.  Methods of measuring green colour and chlorophyll content of apple fruit , 2007 .

[86]  Pierre-Alexandre Vidi,et al.  Plastoglobule lipid bodies: their functions in chloroplasts and their potential for applications. , 2007, Advances in biochemical engineering/biotechnology.

[87]  Jan Kuckenberg,et al.  Evaluation of fluorescence and remission techniques for monitoring changes in peel chlorophyll and internal fruit characteristics in sunlit and shaded sides of apple fruit during shelf-life , 2008 .

[88]  Manuela Zude,et al.  Optical Monitoring of Fresh and Processed Agricultural Crops , 2008 .

[89]  J. V. Aldeeson,et al.  Modern Physical Techniques in Colour Formulation , 2008 .

[90]  M. Merzlyak,et al.  Effect of anthocyanins, carotenoids, and flavonols on chlorophyll fluorescence excitation spectra in apple fruit: signature analysis, assessment, modelling, and relevance to photoprotection. , 2008, Journal of experimental botany.

[91]  C. Kingston Maturity Indices for Apple and Pear , 2010 .