Binding of verocytotoxin 1 to its receptor is influenced by differences in receptor fatty acid content.

Globotriaosylceramide [(Gal alpha 1-4Gal beta 1-4Glc-ceramide (Gb3)] was separated from human kidney, and the fatty acid composition was determined. Semisynthetic Gb3 molecular species of corresponding fatty acid chain length were prepared and compared for verotoxin (VT) binding affinity by TLC overlay, and a quantitative binding assay was performed in the presence of auxiliary lipids. Our results indicate that, within the natural range, fatty acid chain length has little effect on verotoxin binding but that Gb3 molecular species containing different fatty acids can interact to provide a higher affinity toxin receptor than any of the individual component receptor species. Receptor function as assayed by TLC overlay was not always found to correlate with binding in a lipid environment. Short-chain fatty acid Gb3 molecular species could not function as VT receptors under these conditions. Evidence is presented to suggest that fatty acid chain length can have a stereoselective effect on carbohydrate conformation.

[1]  B. Deurs,et al.  Endocytosis and intracellular transport of the glycolipid-binding ligand Shiga toxin in polarized MDCK cells , 1991, The Journal of cell biology.

[2]  C. Lingwood,et al.  Verotoxin-resistant cell clones are deficient in the glycolipid globotriosylceramide: differential basis of phenotype. , 1991, Archives of biochemistry and biophysics.

[3]  C. Lingwood,et al.  Preparation of VT1 and VT2 hybrid toxins from their purified dissociated subunits. Evidence for B subunit modulation of a subunit function. , 1991, The Journal of biological chemistry.

[4]  C. Lingwood,et al.  Induction of verotoxin sensitivity in receptor-deficient cell lines using the receptor glycolipid globotriosylceramide. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[5]  C. Lingwood,et al.  Sensitive receptor-specified enzyme-linked immunosorbent assay for Escherichia coli verocytotoxin , 1989, Journal of Clinical Microbiology.

[6]  S. Ashkenazi,et al.  Rapid method to detect shiga toxin and shiga-like toxin I based on binding to globotriosyl ceramide (Gb3), their natural receptor , 1989, Journal of clinical microbiology.

[7]  G. Keusch,et al.  Pathogenesis of Shigella diarrhea. XIV. Analysis of Shiga toxin receptors on cloned HeLa cells. , 1989, The Journal of infectious diseases.

[8]  J. Brown,et al.  Endocytosis from coated pits of Shiga toxin: a glycolipid-binding protein from Shigella dysenteriae 1 , 1989, The Journal of cell biology.

[9]  M. Karmali,et al.  Infection by verocytotoxin-producing Escherichia coli , 1989, Clinical Microbiology Reviews.

[10]  A. Lindberg,et al.  Studies on the binding of bacteria to glycolipids Two species of Propionibacterium apparently recognize separate epitopes on lactose of lactosylceramide , 1988, FEBS letters.

[11]  G. Keusch,et al.  Pathogenesis of shigella diarrhea: evidence for a developmentally regulated glycolipid receptor for shigella toxin involved in the fluid secretory response of rabbit small intestine. , 1988, The Journal of infectious diseases.

[12]  C. Lingwood,et al.  Globotriosyl ceramide is specifically recognized by the Escherichia coli verocytotoxin 2. , 1988, Biochemical and biophysical research communications.

[13]  B. Williams,et al.  Roles of globotriosyl- and galabiosylceramide in verotoxin binding and high affinity interferon receptor. , 1987, The Journal of biological chemistry.

[14]  T. Yutsudo,et al.  Inhibition of elongation factor 1-dependent aminoacyl-tRNA binding to ribosomes by Shiga-like toxin I (VT1) from Escherichia coli O157:H7 and by Shiga toxin , 1987 .

[15]  J. Mekalanos,et al.  Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[16]  C. Lingwood,et al.  Glycolipid binding of purified and recombinant Escherichia coli produced verotoxin in vitro. , 1987, The Journal of biological chemistry.

[17]  J. Brown,et al.  Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type 1. , 1987, The Journal of biological chemistry.

[18]  J. Boggs,et al.  Factors affecting surface expression of glycolipids: influence of lipid environment and ceramide composition on antibody recognition of cerebroside sulfate in liposomes. , 1986, Biochemistry.

[19]  D. Tyrrell,et al.  Colonic biopsy in verotoxin-induced hemorrhagic colitis and thrombotic thrombocytopenic purpura (TTP). , 1986, American journal of clinical pathology.

[20]  G. Keusch,et al.  Pathogenesis of shigella diarrhea. XI. Isolation of a shigella toxin- binding glycolipid from rabbit jejunum and HeLa cells and its identification as globotriaosylceramide , 1986, The Journal of experimental medicine.

[21]  T. Arima,et al.  Solid-phase radiolabeling of glycolipids with galactose oxidase and sodium boro[3H]hydride. , 1985, Analytical biochemistry.

[22]  C. Pai,et al.  Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features. , 1984, Annals of internal medicine.

[23]  S. Hakomori,et al.  Possible role of ceramide in defining structure and function of membrane glycolipids. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[24]  D Rodbard,et al.  Ligand: a versatile computerized approach for characterization of ligand-binding systems. , 1980, Analytical biochemistry.

[25]  C. Lingwood.  Action of galactose oxidase on galactolipids. , 1979, Canadian journal of biochemistry.

[26]  F. Sharom,et al.  A Glycosphingolipid spin label: Ca2+ effects on sphingolipid distribution in bilayers containing phosphatidyl serine. , 1975, Biochemical and biophysical research communications.

[27]  E. Mårtensson.  Neutral glycolipids of human kidney isolation, identification, and fatty acid composition. , 1966, Biochimica et biophysica acta.

[28]  S. Hakomori.  A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. , 1964, Journal of biochemistry.

[29]  R. Dawson.  A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. , 1960, The Biochemical journal.

[30]  J. Folch,et al.  A simple method for the isolation and purification of total lipides from animal tissues. , 1957, The Journal of biological chemistry.

[31]  P. Strasberg,et al.  Simultaneous fractionation of four placental neutral glycosphingolipids with a continuous gradient. , 1989, Journal of lipid research.

[32]  C. Lingwood,et al.  Verotoxin receptor glycolipid in human renal tissue. , 1989, Nephron.

[33]  H. Lior,et al.  The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. , 1985, The Journal of infectious diseases.

[34]  S. Hakomori.  Glycosphingolipids in cellular interaction, differentiation, and oncogenesis. , 1981, Annual review of biochemistry.