STUDIES ON PRODUCTION OF BIOETHANOL FROM WASTE POTATOES USING CO-CULTURE OF SACCHAROMYCES CEREVISIAE AND ASPERGILLUS NIGER

Bioethanol production from starchy materials such as potato, sweet potato, corn flour is based on bioconversion of starch into sugar and then ethanol by fermentation process. In the present scenario, there is a growing interest for ecologically and economically sustainable biofuel production. In the present scenario, bio-fuels have increased in popularity because of rising oil prices and the need for energy security. India imports >70% of it’s required crude oil, which leads a major expenditure of foreign currency. India can cut it’s crude oil import by using bioethanol for vehicles. In the past years, feasibility of lignocelluloses containing materials for ethyl alcohol production has been explored depending upon the availability in the region (Shindo and Tachibana, 2006). Waste potatoes, as byproduct, are easily available and account for 5-10% of the crop produced in India. The present study was carried out with the objectives of analyze the major chemical constituents of waste potatoes collected from different locations, optimize the fermentation variables for better yield of bioethanol using co-culture of Saccharomyces cerevisiae and Aspergillus niger and quality evaluation of bioethanol produced. In this study for bioethanol production main fermentation variables were optimized in solid state fermentation (SSF) and simultaneous Saccharification and fermentation (SiSF) methods using co-culture of Saccharomyces cerevisiae MTCC 170 and Aspergillus niger MTCC 2196. The results of various experiments revealed that with the SSF technique the highest yield of bioethanol (5.8%) using co-culture of Saccharomyces cerevisiae MTCC 170 and Aspergillus niger MTCC 2196 was obtained at incubation temperature of 30°C after 96 hr of incubation period. In case of simultaneous Saccharification and fermentation (SiSF), the results of various experiments revealed that by employing co-culture of yeast and fungi the highest yield of bioethanol (5.3%) was obtained at a pH of 4.5 with incubation temperature of 25°C after 96 hr of incubation period. The results of various quality attributes of the bioethanol produced showed that there were no major differences in values of density, viscosity, of the bioethanol produced from both methods of fermentation.

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