Review on Impedance Detection of Cellular Responses in Micro/Nano Environment

In general, cell culture-based assays, investigations of cell number, viability, and metabolic activities during culture periods, are commonly performed to study the cellular responses under various culture conditions explored. Quantification of cell numbers can provide the information of cell proliferation. Cell viability study can understand the percentage of cell death under a specific tested substance. Monitoring of the metabolic activities is an important index for the study of cell physiology. Based on the development of microfluidic technology, microfluidic systems incorporated with impedance measurement technique, have been reported as a new analytical approach for cell culture-based assays. The aim of this article is to review recent developments on the impedance detection of cellular responses in micro/nano environment. These techniques provide an effective and efficient technique for cell culture-based assays.

[1]  Qingjun Liu,et al.  Impedance studies of bio-behavior and chemosensitivity of cancer cells by micro-electrode arrays. , 2009, Biosensors & bioelectronics.

[2]  Frank Davis,et al.  Label-free and reversible immunosensor based upon an ac impedance interrogation protocol , 2005 .

[3]  Yanbin Li,et al.  Interdigitated microelectrode (IME) impedance sensor for the detection of viable Salmonella typhimurium. , 2004, Biosensors & bioelectronics.

[4]  Kin Fong Lei,et al.  Electrokinetic DNA concentration in microsystems , 2009 .

[5]  Kin Fong Lei,et al.  Quantitative electrical detection of immobilized protein using gold nanoparticles and gold enhancement on a biochip , 2011 .

[6]  Aaron R Wheeler,et al.  Immunoassays in microfluidic systems , 2010, Analytical and bioanalytical chemistry.

[7]  H. Andersson,et al.  Microfluidic devices for cellomics: a review , 2003 .

[8]  James N Turner,et al.  On-chip micro-biosensor for the detection of human CD4(+) cells based on AC impedance and optical analysis. , 2005, Biosensors & bioelectronics.

[9]  Marc Madou,et al.  DNA hybridization detection by label free versus impedance amplifying label with impedance spectroscopy , 2006 .

[10]  Xuan Weng,et al.  Microfluidic DNA hybridization assays , 2011 .

[11]  S. Quake,et al.  Versatile, fully automated, microfluidic cell culture system. , 2007, Analytical chemistry.

[12]  Rodica Elena Ionescu,et al.  EIS microfluidic chips for flow immunoassay and ultrasensitive cholera toxin detection. , 2011, Lab on a chip.

[13]  Gwo-Bin Lee,et al.  Microfluidic cell culture systems for drug research. , 2010, Lab on a chip.

[14]  Godfrey L. Smith,et al.  Metabolic monitoring of the electrically stimulated single heart cell within a microfluidic platform. , 2006, Lab on a chip.

[15]  Makusu Tsutsui,et al.  Gate manipulation of DNA capture into nanopores. , 2011, ACS nano.

[16]  Liju Yang,et al.  Electrical impedance spectroscopy for detection of bacterial cells in suspensions using interdigitated microelectrodes. , 2008, Talanta.

[17]  Paul C H Li,et al.  Microfluidic DNA microarray analysis: a review. , 2011, Analytica chimica acta.

[18]  Ju Hun Yeon,et al.  Cytotoxicity test based on electrochemical impedance measurement of HepG2 cultured in microfabricated cell chip. , 2005, Analytical biochemistry.

[19]  O. Velev,et al.  On-chip latex agglutination immunoassay readout by electrochemical impedance spectroscopy. , 2012, Lab on a chip.

[20]  E. Alocilja,et al.  A high density microelectrode array biosensor for detection of E. coli O157:H7. , 2005, Biosensors & bioelectronics.

[21]  Chunsun Zhang,et al.  PCR microfluidic devices for DNA amplification. , 2006, Biotechnology advances.

[22]  R. Pethig,et al.  Applications of dielectrophoresis in biotechnology. , 1997, Trends in biotechnology.

[23]  Kenneth M. Yamada,et al.  Taking Cell-Matrix Adhesions to the Third Dimension , 2001, Science.

[24]  Hanry Yu,et al.  Cell Culture on MEMS Platforms: A Review , 2009, International journal of molecular sciences.

[25]  M Javanmard,et al.  A microfluidic platform for electrical detection of DNA hybridization. , 2011, Sensors and actuators. B, Chemical.

[26]  R. Zare,et al.  Microfluidic platforms for single-cell analysis. , 2010, Annual review of biomedical engineering.

[27]  Yanbin Li,et al.  Interdigitated array microelectrodes based impedance biosensors for detection of bacterial cells. , 2009, Biosensors & bioelectronics.

[28]  Kin Fong Lei,et al.  Development of a micro-scale perfusion 3D cell culture biochip with an incorporated electrical impedance measurement scheme for the quantification of cell number in a 3D cell culture construct , 2012 .

[29]  Mayank Thakur,et al.  Real time monitoring of the cell viability during treatment with tumor-targeted toxins and saponins using impedance measurement. , 2012, Biosensors & bioelectronics.

[30]  Albert van den Berg,et al.  Parallel single‐cell analysis microfluidic platform , 2011, Electrophoresis.

[31]  Luke P. Lee,et al.  A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array. , 2005, Lab on a chip.

[32]  Kin Fong Lei,et al.  Microelectrode array biosensor for the detection of Legionellapneumophila , 2012 .

[33]  A. van den Berg,et al.  Monitoring of yeast cell concentration using a micromachined impedance sensor , 2005, The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05..

[34]  Xuezhu Liu,et al.  Electrokinetically controlled DNA hybridization microfluidic chip enabling rapid target analysis. , 2004, Analytical chemistry.

[35]  Kin Fong Lei,et al.  Microfluidic Systems for Diagnostic Applications , 2012, Journal of laboratory automation.

[36]  H. Kimura,et al.  Cell-Based Microfluidic Biochip for Electrochemical Real-Time Monitoring of Glucose and Oxygen , 2007, TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference.

[37]  B Wolf,et al.  Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures. , 1997, Biosensors & bioelectronics.

[38]  F. Rowell,et al.  Use of substrate coated electrodes and AC impedance spectroscopy for the detection of enzyme activity , 1998 .

[39]  Alan Aderem,et al.  A microfluidic device for multiplexed protein detection in nano-liter volumes. , 2009, Analytical biochemistry.

[40]  D. Beebe,et al.  Cell culture models in microfluidic systems. , 2008, Annual review of analytical chemistry.

[41]  Steve Tung,et al.  A Label-free, Microfluidics and Interdigitated Array Microelectrode Based Impedance Biosensor in Combination with Nanoparticles Immunoseparation for Detection of Escherichia coli O157:H7 in Food Samples , 2007 .

[42]  Kin Fong Lei,et al.  Real-time and non-invasive impedimetric monitoring of cell proliferation and chemosensitivity in a perfusion 3D cell culture microfluidic chip. , 2014, Biosensors & bioelectronics.

[43]  C. Culmsee,et al.  Impedance measurement for real time detection of neuronal cell death , 2012, Journal of Neuroscience Methods.

[44]  I. Giaever,et al.  Monitoring fibroblast behavior in tissue culture with an applied electric field. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[45]  Alberto Escarpa,et al.  Electrochemical immunosensing on board microfluidic chip platforms , 2012 .

[46]  A. Abbott.  Cell culture: Biology's new dimension , 2003, Nature.

[47]  C. Klapperich,et al.  Design and testing of a disposable microfluidic chemiluminescent immunoassay for disease biomarkers in human serum samples , 2007, Biomedical microdevices.