Dissolved Carbon Dioxide Sensing Platform for Freshwater and Saline Water Applications: Characterization and Validation in Aquaculture Environments

A sensing configuration for the real-time monitoring, detection, and quantification of dissolved carbon dioxide (dCO2) was developed for aquaculture and other applications in freshwater and saline water. A chemical sensing membrane, based on a colorimetric indicator, is combined with multimode optical fiber and a dual wavelength light-emitting diode (LED) to measure the dCO2-induced absorbance changes in a self-referenced ratiometric scheme. The detection and processing were achieved with an embeded solution having a mini spectrometer and microcontroller. For optrode calibration, chemical standard solutions using sodium carbonate in acid media were used. Preliminary results in a laboratory environment showed sensitivity for small added amounts of CO2 (0.25 mg·L−1). Accuracy and response time were not affected by the type of solution, while precision was affected by salinity. Calibration in freshwater showed a limit of detection (LOD) and a limit of quantification (LOQ) of 1.23 and 1.87 mg·L−1, respectively. Results in saline water (2.5%) showed a LOD and LOQ of 1.05 and 1.16 mg·L−1, respectively. Generally, performance was improved when moving from fresh to saline water. Studies on the dynamics of dissolved CO2 in a recirculating shallow raceway system (SRS+RAS) prototype showed higher precision than the tested commercial sensor. The new sensor is a compact and robust device, and unlike other sensors used in aquaculture, stirring is not required for correct and fast detection. Tests performed showed that this new sensor has a fast accurate detection as well as a strong potential for assessing dCO2 dynamics in aquaculture applications.

[1]  R. Byrne,et al.  Determination of sea water alkalinity by direct equilibration with carbon dioxide , 1992 .

[2]  S. Summerfelt,et al.  Design implication of carbon dioxide production within biofilters contained in recirculating salmonid culture systems , 2004 .

[3]  A new highly sensitive and versatile optical sensing film for controlling CO2 in gaseous and aqueous media , 2013 .

[4]  G. Gerlach,et al.  Carbon Dioxide Sensing , 2019 .

[5]  Andrew Mills,et al.  Optical sensors for carbon dioxide and their applications , 2009 .

[6]  S. Gelcich,et al.  Ocean Acidification, Consumers' Preferences, and Market Adaptation Strategies in the Mussel Aquaculture Industry , 2019, Ecological Economics.

[7]  P. Caldas,et al.  Fiber modal Michelson interferometers with coherence addressing and heterodyne interrogation , 2008 .

[8]  Digvir S. Jayas,et al.  Carbon Dioxide (CO2) Sensors for the Agri-food Industry—A Review , 2009 .

[9]  C. Gouveia,et al.  Characterization of a novel dissolved CO2 sensor for utilization in environmental monitoring and aquaculture industry , 2013, Iberoamerican Meeting of Optics and the Latin American Meeting of Optics, Lasers and Their Applications.

[10]  Ingo Klimant,et al.  Microsecond lifetime-based optical carbon dioxide sensor using luminescence resonance energy transfer , 1999 .

[11]  A. Mills,et al.  Extruded colour-based plastic film for the measurement of dissolved CO2 , 2016 .

[12]  P. Bühlmann,et al.  Carrier-Based Ion-Selective Electrodes and Bulk Optodes. 2. Ionophores for Potentiometric and Optical Sensors. , 1998, Chemical reviews.

[13]  An Autonomous Sensor and Telemetry System for Low-Level pCO(2) Measurements in Seawater. , 1999, Analytical chemistry.

[14]  R. Wanninkhof,et al.  Simultaneous spectrophotometric flow-through measurements of pH, carbon dioxide fugacity, and total inorganic carbon in seawater. , 2007, Analytica chimica acta.

[15]  O. Wolfbeis.  Fiber-optic sensors in bioprocess control. , 1990, Bioprocess technology.

[16]  Per O.J. Hall,et al.  Performance of a lifetime‐based optode for measuring partial pressure of carbon dioxide in natural waters , 2014 .

[17]  David R. Walt,et al.  A Fiber-Optic Carbon Dioxide Sensor for Fermentation Monitoring , 1995, Bio/Technology.

[18]  D. Moran.  Carbon dioxide degassing in fresh and saline water I: degassing performance of a cascade column , 2010 .

[19]  P. Williamson,et al.  The Rise of CO 2 and Ocean Acidification , 2013 .

[20]  R. Weiss.  Determinations of Carbon Dioxide and Methane by Dual Catalyst Flame Ionization Chromatography and Nitrous Oxide by Electron Capture Chromatography , 1981 .

[21]  E. Eek,et al.  Automatic method for the determination of total CO2 in natural waters , 1995 .

[22]  Mark T. Stauffer.  Calibration and Validation of Analytical Methods - A Sampling of Current Approaches , 2018 .

[23]  P. Witten,et al.  Impact of high water carbon dioxide levels on Atlantic salmon smolts (Salmo salar L.) : effects on fish performance, vertebrae composition and structure , 2006 .

[24]  A. Tengberg,et al.  The use of novel optode sensor technologies for monitoring dissolved carbon dioxide and ammonia concentrations under live haul conditions , 2017 .

[25]  Jorge O. Domingues,et al.  Direct and continuous dissolved CO2 monitoring in shallow raceway systems: From laboratory to commercial-scale applications , 2012 .

[26]  D. Diamond,et al.  Towards an autonomous microfluidic sensor for dissolved carbon dioxide determination , 2018, Microchemical Journal.

[27]  T. Losordo,et al.  Recirculating Aquaculture , 2006 .

[28]  Jens Zosel,et al.  The measurement of dissolved and gaseous carbon dioxide concentration , 2011 .

[29]  O. Wolfbeis.  Fiber-optic chemical sensors and biosensors. , 2004, Analytical chemistry.

[30]  Barnaby J. Watten,et al.  Comparative performance of CO2 measuring methods: Marine aquaculture recirculation system application , 2011 .

[31]  Kerry J. Dinsmore,et al.  Direct and continuous measurement of dissolved carbon dioxide in freshwater aquatic systems—method and applications , 2010 .

[32]  Taro Takahashi,et al.  Amperometric sensor for monitoring of dissolved carbon dioxide in seawater , 2001 .

[33]  A. Barnard,et al.  Optical tools for ocean monitoring and research , 2008 .

[34]  G. Kwak,et al.  Colorimetric fluorescence response to carbon dioxide using charge transfer dye and molecular rotor dye in smart solvent system , 2016 .

[35]  K. Johnson,et al.  A validation and comparison study of new, compact, versatile optodes for oxygen, pH and carbon dioxide in marine environments , 2018, Marine Chemistry.