A highly sensitive electrochemical immunosensor based on coral-shaped AuNPs with CHITs inorganic-organic hybrid film.

A highly sensitive electrochemical immunosensor based on combination of chitosan (CHIT) and coral-shaped AuNPs (C-AuNPs) to form an immobilization matrix has been developed using human IgG as a model analyte. The inorganic-organic hybrid film with abundant adsorbing sites and large surface area can reserve the biocompatibility of the biomaterials which greatly increase loading amounts of assembling, thus, significantly improves the performance of biosensing. The morphology is studied by scanning electron microscopy (SEM). Under the optimized experimental conditions, the immunosensor exhibits excellent performance (e.g., a detection limit of 5 pmol L(-1), a linear dynamic range of 3 orders of magnitude, high specificity). This possibly makes it an attractive platform for the direct immunoassay of human IgG or other biomolecules.

[1]  Yi Xiao,et al.  Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. , 2004, Journal of the American Chemical Society.

[2]  Y. Wan,et al.  An enzyme-based E-DNA sensor for sequence-specific detection of femtomolar DNA targets. , 2008, Journal of the American Chemical Society.

[3]  C. Murphy,et al.  Room temperature, high-yield synthesis of multiple shapes of gold nanoparticles in aqueous solution. , 2004, Journal of the American Chemical Society.

[4]  G. Shen,et al.  A novel immunoassay strategy based on combination of chitosan and a gold nanoparticle label. , 2007, Talanta.

[5]  Qinglin Sheng,et al.  Enzymatically induced formation of neodymium hexacyanoferrate nanoparticles on the glucose oxidase/chitosan modified glass carbon electrode for the detection of glucose. , 2008, Biosensors & bioelectronics.

[6]  Jianzhong Lu,et al.  Sequential determination of two proteins by temperature-triggered homogeneous chemiluminescent immunoassay. , 2006, Analytical chemistry.

[7]  G. Shen,et al.  Seed-mediated growth of platinum nanoparticles on carbon nanotubes for the fabrication of electrochemical biosensors , 2008 .

[8]  C. Murphy,et al.  Anisotropic metal nanoparticles: Synthesis, assembly, and optical applications. , 2005, The journal of physical chemistry. B.

[9]  Guo-Li Shen,et al.  Platinum nanoparticle-modified carbon fiber ultramicroelectrodes for mediator-free biosensing , 2006 .

[10]  D. Astruc,et al.  Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. , 2004, Chemical reviews.

[11]  J. Storhoff,et al.  A DNA-based method for rationally assembling nanoparticles into macroscopic materials , 1996, Nature.

[12]  Vincent M Rotello,et al.  Gold nanoparticles in delivery applications. , 2008, Advanced drug delivery reviews.

[13]  G. Shen,et al.  Surface attached-poly(acrylic acid) network as nanoreactor to in-situ synthesize palladium nanoparticles for H2O2 sensing , 2009 .

[14]  Yuehe Lin,et al.  Versatile apoferritin nanoparticle labels for assay of protein. , 2006, Analytical chemistry.

[15]  Huangxian Ju,et al.  Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer , 1999 .

[16]  John B. Shoven,et al.  I , Edinburgh Medical and Surgical Journal.

[17]  Suiping Wang,et al.  A novel electrochemical immunosensor based on ordered Au nano-prickle clusters. , 2008, Biosensors & bioelectronics.

[18]  J. Savéant,et al.  Quantitative analysis of catalysis and inhibition at horseradish peroxidase monolayers immobilized on an electrode surface. , 2003, Journal of the American Chemical Society.

[19]  C. Suri,et al.  Facile synthesis and functionalization of water-soluble gold nanoparticles for a bioprobe. , 2008, Analytica chimica acta.

[20]  P. He,et al.  Colloid Au-enhanced DNA immobilization for the electrochemical detection of sequence-specific DNA , 2001 .

[21]  W. Bentley,et al.  Chitosan-coated wires: conferring electrical properties to chitosan fibers. , 2009, Biomacromolecules.

[22]  H. Luo,et al.  Thermodynamic and kinetic analysis of the interaction between hepatitis B surface antibody and antigen on a gold electrode modified with cysteamine and colloidal gold via electrochemistry. , 2007, Biosensors & bioelectronics.

[23]  Arben Merkoçi,et al.  Double-codified gold nanolabels for enhanced immunoanalysis. , 2007, Analytical chemistry.

[24]  Chih-Ching Huang,et al.  Separation of long double-stranded DNA by nanoparticle-filled capillary electrophoresis. , 2004, Analytical chemistry.

[25]  G. Shen,et al.  Biocatalytic growth of gold agglomerates on an electrode for aptamer-based electrochemical detection. , 2010, The Analyst.

[26]  I. Willner,et al.  Organization of Au Colloids as Monolayer Films onto ITO Glass Surfaces: Application of the Metal Colloid Films as Base Interfaces To Construct Redox-Active Monolayers , 1995 .