论文信息 - Triplex Virion Capture (VC)/RT-PCR for Three Seed Transmissible Tobamoviruses of CGMMV, ZGMMV and KGMMV Occurring on Cucurbitaceae

Triplex Virion Capture (VC)/RT-PCR for Three Seed Transmissible Tobamoviruses of CGMMV, ZGMMV and KGMMV Occurring on Cucurbitaceae

The genetic diagnostic method of virion capture (VC)/RT-PCR was developed for the simultaneous detection of three rod shaped viruses of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus(KGMMV) and Zucchini green mottle mosaic virus (ZGMMV) transmitted by seed in Cucurbit. Out of 12 primer combinations for the three tobamoviruses, a primer set of CGMMV-C724, KGMMV-K513 and ZGMMV-Z407A was useful for mono and triplex VC/RT-PCR. The triplex VC/RT-PCR for the three tobamovirus in Cucurbit could detect specifically without interference among primers and/or plant species of watermelon, gourd, cucumber, melon, pumpkin, squash and Nicotiana benthamiana.

Jeong-Soo Kim | B. Chung | Jeom-Deog Cho | Sin-Ho Lee

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