Visual Object Tracking for the Extraction of Multiple Interacting Plant Root Systems

We propose a visual object tracking framework for the extraction of multiple interacting plant root systems from three-dimensional X-ray micro computed tomography images of plants grown in soil. Our method is based on a level set framework guided by a greyscale intensity distribution model to identify object boundaries in image cross-sections. Root objects are followed through the data volume, while updating the tracker’s appearance models to adapt to changing intensity values. In the presence of multiple root systems, multiple trackers can be used, but need to distinguish target objects from one another in order to correctly associate roots with their originating plants. Since root objects are expected to exhibit similar greyscale intensity distributions, shape information is used to constrain the evolving level set interfaces in order to lock trackers to their correct targets. The proposed method is tested on root systems of wheat plants grown in soil.

[1]  J. Lynch,et al.  Shovelomics: high throughput phenotyping of maize (Zea mays L.) root architecture in the field , 2011, Plant and Soil.

[2]  J. Alison Noble,et al.  An adaptive segmentation algorithm for time-of-flight MRA data , 1999, IEEE Transactions on Medical Imaging.

[3]  J. Lynch Root Architecture and Plant Productivity , 1995, Plant physiology.

[4]  D. Chopp Computing Minimal Surfaces via Level Set Curvature Flow , 1993 .

[5]  D. Aykac,et al.  Segmentation and analysis of the human airway tree from three-dimensional X-ray CT images , 2003, IEEE Transactions on Medical Imaging.

[6]  Alex M. Andrew,et al.  Level Set Methods and Fast Marching Methods: Evolving Interfaces in Computational Geometry, Fluid Mechanics, Computer Vision, and Materials Science (2nd edition) , 2000 .

[7]  Milan Sonka,et al.  Rule-based detection of intrathoracic airway trees , 1996, IEEE Trans. Medical Imaging.

[8]  Baba C. Vemuri,et al.  Shape Modeling with Front Propagation: A Level Set Approach , 1995, IEEE Trans. Pattern Anal. Mach. Intell..

[9]  Nicolas Flasque,et al.  Acquisition, segmentation and tracking of the cerebral vascular tree on 3D magnetic resonance angiography images , 2001, Medical Image Anal..

[10]  James A. Sethian,et al.  Level Set Methods and Fast Marching Methods: Evolving Interfaces in Computational Geometry, Fluid , 2012 .

[11]  Frank Dellaert,et al.  MCMC-based particle filtering for tracking a variable number of interacting targets , 2005, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[12]  Hongkai Zhao,et al.  A fast sweeping method for Eikonal equations , 2004, Math. Comput..

[13]  R. MacCurdy,et al.  Three-Dimensional Root Phenotyping with a Novel Imaging and Software Platform1[C][W][OA] , 2011, Plant Physiology.

[14]  J. Hopmans,et al.  Three dimensional imaging of plant roots in situ with X-ray Computed Tomography , 1997, Plant and Soil.

[15]  Anders Kaestner,et al.  Visualizing three-dimensional root networks using computed tomography , 2006 .

[16]  Gösta H. Granlund,et al.  Fourier Preprocessing for Hand Print Character Recognition , 1972, IEEE Transactions on Computers.

[17]  Erik Meijering,et al.  Neuron tracing in perspective , 2010, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[18]  Anne Lohrli Chapman and Hall , 1985 .

[19]  Paul J. Besl,et al.  A Method for Registration of 3-D Shapes , 1992, IEEE Trans. Pattern Anal. Mach. Intell..

[20]  J. Perret,et al.  Non-destructive visualization and quantification of roots using computed tomography , 2007 .

[21]  J. Sethian Level Set Techniques for Tracking Interfaces ; Fast Algorithms , Multiple Regions , Grid Generation , and Shape / Character Recognition , 2006 .

[22]  Azriel Rosenfeld,et al.  Connectivity in Digital Pictures , 1970, JACM.

[23]  Chris Moran,et al.  X-ray computed tomography to quantify tree rooting spatial distributions , 1999 .

[24]  S. Mooney,et al.  Developing X-ray Computed Tomography to non-invasively image 3-D root systems architecture in soil , 2011, Plant and Soil.

[25]  Marc Levoy,et al.  Efficient variants of the ICP algorithm , 2001, Proceedings Third International Conference on 3-D Digital Imaging and Modeling.

[26]  Paul L. Rosin Unimodal thresholding , 2001, Pattern Recognit..

[27]  Joshua S. Weitz,et al.  Leaf Extraction and Analysis Framework Graphical User Interface: Segmenting and Analyzing the Structure of Leaf Veins and Areoles1[W][OA] , 2010, Plant Physiology.

[28]  Jianhua Lin,et al.  Divergence measures based on the Shannon entropy , 1991, IEEE Trans. Inf. Theory.

[29]  S. Mooney,et al.  Effects of X-Ray Dose On Rhizosphere Studies Using X-Ray Computed Tomography , 2013, PloS one.

[30]  Milan Sonka,et al.  Intrathoracic airway trees: segmentation and airway morphology analysis from low-dose CT scans , 2005, IEEE Transactions on Medical Imaging.

[31]  Philippe Lucidarme,et al.  On the use of depth camera for 3D phenotyping of entire plants , 2012 .

[32]  Luc Vincent,et al.  Morphological grayscale reconstruction in image analysis: applications and efficient algorithms , 1993, IEEE Trans. Image Process..

[33]  Milan Sonka,et al.  Image Processing, Analysis and Machine Vision , 1993, Springer US.

[34]  Shiv O. Prasher,et al.  Advances in the acquisition and analysis of CT scan data to isolate a crop root system from the soil medium and quantify root system complexity in 3-D space , 2006 .

[35]  A. Greenberg,et al.  High-Resolution Inflorescence Phenotyping Using a Novel Image-Analysis Pipeline, PANorama1[W][OPEN] , 2014, Plant Physiology.

[36]  Peter J. Gregory,et al.  Rhizosphere geometry and heterogeneity arising from root-mediated physical and chemical processes. , 2005, The New phytologist.

[37]  H. Schenk,et al.  Root competition: beyond resource depletion , 2006 .