Lab-on-a-chip rendszerek a betegágy melletti diagnosztikában

The need in modern medicine for near-patient diagnostics being able to accelerate therapeutic decisions and possibly replacing laboratory measurements is significantly growing. Reliable and cost-effective bioanalytical measurement systems are required which - acting as a micro-laboratory - contain integrated biomolecular recognition, sensing, signal processing and complex microfluidic sample preparation modules. These micro- and nanofabricated Lab-on-a-chip systems open new perspectives in the diagnostic supply chain, since they are able even for quantitative, high-precision and immediate analysis of special disease specific molecular markers or their combinations from a single drop of sample. Accordingly, crucial requirements regarding the instruments and the analytical methods are the high selectivity, extremely low detection limit, short response time and integrability into the healthcare information networks. All these features can make the hierarchical examination chain shorten, and revolutionize laboratory diagnostics, evolving a brand new situation in therapeutic intervention.

[1]  Suhas S. Joshi,et al.  Passive blood plasma separation at the microscale: a review of design principles and microdevices , 2015 .

[2]  Róbert E. Gyurcsányi,et al.  Chemically-modified nanopores for sensing , 2008 .

[3]  C. Borrebaeck,et al.  Antibodies in diagnostics - from immunoassays to protein chips. , 2000, Immunology today.

[4]  D. Beebe,et al.  The present and future role of microfluidics in biomedical research , 2014, Nature.

[5]  Kristen L. Helton,et al.  Microfluidic Overview of Global Health Issues Microfluidic Diagnostic Technologies for Global Public Health , 2006 .

[6]  Péter Fürjes,et al.  Optimized Simulation and Validation of Particle Advection in Asymmetric Staggered Herringbone Type Micromixers , 2014, Micromachines.

[7]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[8]  G. Whitesides.  The origins and the future of microfluidics , 2006, Nature.

[9]  Chun-Xia Zhao,et al.  Two-phase microfluidic flows , 2011 .

[10]  P. Fürjes,et al.  Performance characterization of micromachined particle separation system based on Zweifach–Fung effect , 2012 .

[11]  P. Sajeesh,et al.  Particle separation and sorting in microfluidic devices: a review , 2014 .

[12]  M. Roukes,et al.  Comparative advantages of mechanical biosensors. , 2011, Nature nanotechnology.

[13]  Guo-Jun Zhang,et al.  Silicon nanowire biosensor and its applications in disease diagnostics: a review. , 2012, Analytica chimica acta.

[14]  S. Jayasena.  Aptamers: an emerging class of molecules that rival antibodies in diagnostics. , 1999, Clinical chemistry.

[15]  P. Fürjes,et al.  PDMS microfluidics developed for polymer based photonic biosensors , 2015 .

[16]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[17]  Junjie Zhu,et al.  Particle focusing in microfluidic devices , 2010 .

[18]  R. Stoltenburg,et al.  SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands. , 2007, Biomolecular engineering.