Distinct pathways for jasmonate‐ and elicitor‐induced expressions of a cytochrome P450 gene in soybean suspension‐cultured cells

The mechanisms of the jasmonate-induced expression of genes encoding the cytochrome P450 CYP93A1 and lipoxygenase L-4 were analyzed in a soybean photomix-otrophic cultured cell line, SB-P. The induction of the cytochrome P450 gene caused by methyl jasmonate (MeJA) was specifically suppressed by trifluoperazine and DCMU, inhibitors of chloroplast electron transport. Additionally, induction of the cytochrome P450 gene required irradiation. In contrast, induction of the lipoxygenase L-4 gene by the MeJA treatment occurred in both dark and light. Based on the results, the presence of two distinct signalling pathways for jasmonate-inducible gene expression, light-dependent and light-independent, is proposed. The jasmonate-inducible cytochrome P450 was also specifically induced by a fungal elicitor from a cell wall fraction of Phytophthora megasperma, a fungal pathogen, suggesting a role for P450 in the defense response to fungus in soybean cells. However, trifluoperazine did not block the elicitor-induced expression of cytochrome P450.

[1]  C. Douglas,et al.  Role of Jasmonates in the Elicitor- and Wound-Inducible Expression of Defense Genes in Parsley and Transgenic Tobacco , 1996, Plant physiology.

[2]  D. Shibata.  Plant Lipoxygenase Genes , 1996 .

[3]  D. Shibata,et al.  Induction of a novel cytochrome P450 (CYP93 family) by methyl jasmonate in soybean suspension-cultured cells. , 1996, FEBS letters.

[4]  Y. Okinaka,et al.  A Structural Model for the Mechanisms of Elicitor Release from Fungal Cell Walls by Plant [beta]-1,3-Endoglucanase , 1995, Plant physiology.

[5]  G. Bolwell,et al.  Plant cytochrome P450. , 1994, Phytochemistry.

[6]  K. Skriver,et al.  The barley 60 kDa jasmonate-induced protein (JIP60) is a novel ribosome-inactivating protein. , 1994, The Plant journal : for cell and molecular biology.

[7]  N. Chua,et al.  Phytochrome signal transduction pathways are regulated by reciprocal control mechanisms. , 1994, Genes & development.

[8]  S. Reinbothe,et al.  JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens. , 1994, The Plant cell.

[9]  K. Apel,et al.  JIP60, a methyl jasmonate-induced ribosome-inactivating protein involved in plant stress reactions. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[10]  G. May,et al.  Phosphate Modulates Transcription of Soybean VspB and Other Sugar-Inducible Genes. , 1994, The Plant cell.

[11]  R. J. Aerts,et al.  Methyl jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus and Cinchona seedlings , 1994 .

[12]  Yoshikazu Tanaka,et al.  Cloning and expression of cytochrome P450 genes controlling flower colour , 1993, Nature.

[13]  D. Shibata,et al.  Soybean Lipoxygenase L-4, a Component of the 94-kilodalton Storage Protein in Vegetative Tissues: Expression and Accumulation in Leaves Induced by Pod Removal and by Methyl Jasmonate , 1993 .

[14]  M. Zenk,et al.  Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Zenk,et al.  The jasmonate precursor, 12‐oxo‐phytodienoic acid. Induces phytoalexin synthesis in Petroselinum crispum cell cultures , 1992, FEBS letters.

[16]  M. Zenk,et al.  Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[17]  D. Hildebrand,et al.  The soybean 94-kilodalton vegetative storage protein is a lipoxygenase that is localized in paraveinal mesophyll cell vacuoles. , 1991, The Plant cell.

[18]  T. Boller,et al.  Elicitor-induced ethylene biosynthesis in tomato cells: characterization and use as a bioassay for elicitor action. , 1991, Plant physiology.

[19]  E. Farmer,et al.  Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[20]  H. Yu,et al.  Sequence analysis of ripening-related cytochrome P-450 cDNAs from avocado fruit. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[21]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[22]  J. Widholm,et al.  Photoautotrophic growth of soybean cells in suspension culture: I. Establishment of photoautotrophic cultures. , 1983, Plant physiology.

[23]  F. L. Crane,et al.  Calmodulin antagonists inhibit electron transport in photosystem II of spinach chloroplasts. , 1982, Biochemical and biophysical research communications.

[24]  P. Albersheim,et al.  Host-Pathogen Interactions: XIV. Isolation and Partial Characterization of an Elicitor from Yeast Extract. , 1978, Plant physiology.

[25]  B. Parthier,et al.  The Biochemistry and the Physiological and Molecular Actions of Jasmonates , 1993 .

[26]  K. Bozak.  Sequence analysis of ripening-related cytochrom P450 form avacado fruit. , 1990 .

[27]  V. Franceschi,et al.  Jasmonic acid-dependent increase in the level of vegetative storage proteins in soybean , 1989 .

[28]  D W Nebert,et al.  P450 genes: structure, evolution, and regulation. , 1987, Annual review of biochemistry.

[29]  J. Salaün,et al.  Phytochrome-mediated regulation of a monooxygenase hydroxylating cinnamic acid in etiolated pea seedlings , 1978 .