Microbial properties, enzyme activities and the persistence of exogenous proteins in soil under consecutive cultivation of transgenic cottons (Gossypium hirsutum L.)

One Bacillus thuringiensis (Bt) and two stacked Bt and cowpea trypsin inhibitor (Bt + CpTI) cottons and their non-transgenic isolines were consecutively cultivated to investigate the soil persistence of Cry1Ac and CpTI proteins and their effects on microbial properties and enzyme activities involving C, N, P, and S cycling in soil. Results showed that there were the persistence of Cry1Ac and CpTI proteins in soil under 4-year consecutive cultivation of transgenic cottons. Cry1Ac proteins varied from 6.75 ng/g to 12.01 ng/g and CpTI proteins varied from 30.65 to 43.60 ng/g. However, neither of these two proteins was detected in soil under non-transgenic cottons. Soil microbial biomass carbon, microbial activities, and soil enzyme activities (except urease and phosphodiesterase) signifi cantly decreased in soil under transgenic cottons. Correlation analysis showed that most of microbial properties and enzyme activities in soil had a negative relationship with Cry1Ac content, while most of them had a positive relationship with CpTI content. Our data indicate that consecutive cultivation by genetically modified cottons with Bt and CpTI genes can result in persistence of Cry1Ac and CpTI proteins and negatively affect soil microbial and biochemical properties.

[1]  W. Horwath,et al.  Microbial Biomass , 2018, SSSA Book Series.

[2]  T. J. Purakayastha,et al.  Assessment of biological and biochemical indicators in soil under transgenic Bt and non-Bt cotton crop in a sub-tropical environment , 2009, Environmental monitoring and assessment.

[3]  D. Andow,et al.  Is the Cry1Ab Protein from Bacillusthuringiensis (Bt) Taken Up by Plants from Soils Previously Planted with Bt Corn and by Carrot from Hydroponic Culture? , 2009, Bulletin of environmental contamination and toxicology.

[4]  Hai-yan Hu,et al.  Effects of repeated cultivation of transgenic Bt cotton on functional bacterial populations in rhizosphere soil , 2009 .

[5]  Feng Yuanjiao.  Effects of straw return pattern on the degradation of Bt protein released from Bt corns in field , 2009 .

[6]  G. Stotzky,et al.  Fate and effects of insect-resistant Bt crops in soil ecosystems , 2008 .

[7]  D. Saxena,et al.  Microbial populations and enzyme activities in soil in situ under transgenic corn expressing cry proteins from Bacillus thuringiensis. , 2008, Journal of environmental quality.

[8]  F. Achuba,et al.  Effect of spent engine oil on soil catalase and dehydrogenase activities , 2008 .

[9]  G. Stotzky,et al.  Cry3Bb1 protein from Bacillus thuringiensis in root exudates and biomass of transgenic corn does not persist in soil , 2008, Transgenic Research.

[10]  Liu Zhaohu,et al.  Transgenic cotton could safely be grown since CpTI toxin rapidly degrades in the rhizosphere soil , 2007 .

[11]  W. Xiao.  Effect of planting transgenic Bt cotton on soil enzymatic and microbial activities , 2007 .

[12]  C. X. Sun,et al.  Soil persistence of Bacillus thuringiensis (Bt) toxin from transgenic Bt cotton tissues and its effect on soil enzyme activities , 2007, Biology and Fertility of Soils.

[13]  Ren Fang Shen,et al.  Transgenic Bt cotton has no apparent effect on enzymatic activities or functional diversity of microbial communities in rhizosphere soil , 2006, Plant and Soil.

[14]  V. S. Greena,et al.  Assay for fluorescein diacetate hydrolytic activity : Optimization for soil samples , 2006 .

[15]  F. Gagné,et al.  Tracking the source of Bacillus thuringiensis Cry1Ab endotoxin in the environment , 2005 .

[16]  Zhaohu Li,et al.  Changes of Bt Toxin in the Rhizosphere of Transgenic Bt Cotton and its Influence on Soil Functional Bacteria , 2005 .

[17]  W. Gams.  Soil enzymes , 1973, Netherlands Journal of Plant Pathology.

[18]  Shaoyu Guo,et al.  Feeding behaviour of Helicoverpa armigera larvae on insect‐resistant transgenic cotton and non‐transgenic cotton , 2004 .

[19]  E. Gregorich,et al.  Detection and decay of the Bt endotoxin in soil from a field trial with genetically modified maize , 2003 .

[20]  A. Hilbeck,et al.  Degradation of the Cry1Ab protein within transgenic Bacillus thuringiensis corn tissue in the field , 2003, Molecular ecology.

[21]  Graham P. Head,et al.  No Detection of Cry1Ac Protein in Soil After Multiple Years of Transgenic Bt Cotton (Bollgard) Use , 2002 .

[22]  G. Adam,et al.  Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils , 2001 .

[23]  G. Stotzky,et al.  Biodegradation and insecticidal activity of the toxin from Bacillus thuringiensis subsp. kurstaki bound on complexes of montmorillonite–humic acids–Al hydroxypolymers , 2001 .

[24]  C. Trasar-Cepeda,et al.  An improved method to measure catalase activity in soils , 1999 .

[25]  H. Tapp,et al.  Persistence of the insecticidal toxin from Bacillus thuringiensis subsp. kurstaki in soil , 1998 .

[26]  H. Tapp,et al.  Insecticidal Activity of the Toxins from Bacillus thuringiensis subspecies kurstaki and tenebrionis Adsorbed and Bound on Pure and Soil Clays , 1995, Applied and environmental microbiology.

[27]  T. Rosswall,et al.  Fluorescein Diacetate Hydrolysis as a Measure of Total Microbial Activity in Soil and Litter , 1982, Applied and environmental microbiology.

[28]  J. Ladd,et al.  Short-term assays of soil proteolytic enzyme activities using proteins and dipeptide derivatives as substrates , 1972 .