GAS AND HIGH‐PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSES OF AMINO ACIDS IN SOYBEAN MEAL, CORN AND TRITICALE

Analyses of amino acids in six samples each of soybean meal, corn and triticale by high-performance liquid chromatography (HPLC) and gas chromatography (GC) were compared. Samples were hydrolyzed in 6N HC1, then phenylthiocarbamyl (for HPLC) and N-heptafluorobutyryl (for GC) derivatives of free amino acids were prepared and analyzed by the appropriate system. Coefficient of variation within each feedstuff for amino acids analyzed by HPLC ranged from 1.8 (leucine) to 23.5% (methionine) in soybean meal, 2.7 (leucine) to 14.7% (methionine) in corn and 1.7 (leucine) to 14.3% (cystine) in triticale. Corresponding figures from GC analysis were 0.7 to 22.4%, 1.4 to 14.3% and 1.6 to 12.1%, respectively. Linear regression of HPLC on GC values gave good fits with R2 ranging from 0.86 for cystine and 0.99 for isoleucine, leucine, lysine, methionine, phenylalanine, threonine, alanine, aspartic acid and glycine. Although some differences between the two systems were evident, feedstuff amino acid values obtained by HPLC and GC analyses compare very well. Results of this study and consideration of such factors as ease of derivatization and length of time for analysis, in our opinion, make HPLC the system of choice.

[1]  D. Hallford,et al.  Rapid [14C]heptachlor clearance from body stores of ovines: ingested mineral oil and parenteral trans-stilbene oxide lack effects. , 1989, Journal of animal science.

[2]  I. Mcmillan,et al.  OAC WINTRI TRITICALE IN DIETS OF GROWING SWINE , 1987 .

[3]  O. M. Hale,et al.  Value of Beagle 82 Triticale as a Substitute for Corn and Soybean Meal in the Diet of Pigs , 1985 .

[4]  O. M. Hale,et al.  Nutritive Value of Beagle 82 Triticale for Swine , 1985 .

[5]  B. Bidlingmeyer,et al.  Rapid analysis of amino acids using pre-column derivatization. , 1984, Journal of chromatography.

[6]  P. Thacker,et al.  Amino Acid Availabilities in Soybean Meal, Sunflower Meal, Fish Meal and Meat and Bone Meal Fed to Growing Pigs , 1984 .

[7]  R. Heinrikson,et al.  Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate. , 1984, Analytical biochemistry.

[8]  G. Shephard,et al.  Gas chromatographic analysis of amino acids in physiological fluids: a critique. , 1984, Journal of chromatography.

[9]  S. Mackenzie,et al.  Quantitative formation of N(O,S)-heptafluorobutyryl isobutyl amino acids for gas chromatographic analysis , 1979 .

[10]  R. J. Pearce.  Amino acid analysis by gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters. Complete resolution using a support-coated open-tubular capillary column. , 1977, Journal of chromatography.

[11]  J. March.  A modified technique for the quantitative analysis of amino acids by gas chromatography using heptafluorobutyric n-propyl derivatives. , 1975, Analytical biochemistry.

[12]  M. A. Kirkman.  Comparative determination of protein amino acids in plant materials by automated cation exchange and gas-liquid chromatography of the amino acid N-heptafluorobutyryl, n-propyl esters. , 1974, Journal of chromatography.

[13]  S. Mackenzie,et al.  Gas-liquid chromatography of N-heptafluorobutyryl isobutyl esters of amino acids. , 1974, Journal of chromatography.

[14]  K. Kuo,et al.  Quantitative amino acid analysis by gas-liquid chromatography. , 1971, Journal of agricultural and food chemistry.

[15]  S. Moore,et al.  Chromatography of amino acids on sulfonated polystyrene resins. , 1951, The Journal of biological chemistry.