Fluorescence Spectroscopy Data Analysis Environment: A Second Generation Global Analysis Program

A fluorescence spectroscopy global analysis environment is described. Within this analysis environment, multidimensional fluorescence decay data (time and frequency domains) can be analyzed in terms of a wide variety of photophysical models. A generalized compartmental analysis structure is utilized, where one can specify the functions used to link the various compartments together. All fitting parameters may be characterized by either discrete or distributed values. Multi-temperature global analysis of frequency domain fluorescence studies from a single tryptophan containing protein are described.

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