Development and Characterization of Mouse Hyperplastic Mammary Outgrowth Lines from BALB / cfC 3 H Hyperplastic Alveolar Nodules 1

Five hyperplastic outgrowth lines were developed by serial transplantation of hyperplastic alveolar nodules from BALB/ cfC3H mice into BALB/c hosts. The lines were used to study the biology, morphology, and virology of premalignant tissue originating in mouse mammry tumor virus (MuMTV)-positive animals. The five lines differed with respect to tumor potential and growth characteristics, corroborating the previous evi dence that MuMTV-positive hyperplastic alveolar nodules are biologically heterogeneous. Subgross and microscopic exam ination of outgrowths and tumors revealed that each line had unique morphological characteristics. The presence of atypical lobules within the hyperplastic outgrowth appeared to be cor related with tumor risk, and a morphological continuum of atypical lesions ending in overt cancer was suggested. Viral expression was detected by nucleic acid hybridization and ¡mmunoperoxidase staining for MuMTV structural anti gens. While the MuMTV RNA in certain tissues appeared to vary qualitatively with tumor potential of the outgrowth line, no correlation between viral antigens detected by ¡mmunoperoxi dase staining and tumor potential was observed.

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