R 4 S Mutations Are Rare Events in Philadelphia Chromosome-Negativelbcr Gene Rearrangement-Negative Chronic Myelogenous Leukemia , But Are Prevalent in Chronic Myelomonocytic Leukemia

Previous reports have indicated that mutations of the RAS oncogenes are not associated with the chronic phase of Philadelphia chromosome-positive chronic myelogenous leukemia (Ph’ + CML). However, further studies were needed to determine their association with Ph’CML and chronic myelomonocytic leukemia (CMML). Therefore, 6 patients with Ph’CML who were also negative for BCR rearrangements (Ph’IBCRCML) and 30 patients with CMML were analyzed for the presence of RAS oncogene point mutations to determine the similarities of these diseases at the molecular level. The assay used the polymerase chain reaction for amplification of the target RAS sequences and panels of specific synthetic oligonucleotide probes for hybridization to wild type and/or mutated sequences. None of the six Ph’-IBCRCML patients had mutations in the RAS oncogenes, while 17 of 30 (57%) of the CMML

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