Nondestructive diagnostics of magnesium deficiency based on distribution features of chlorophyll concentrations map on cucumber leaf

Abstract A new and nondestructive method for diagnosing magnesium (Mg) deficiency based on chlorophyll concentration distribution features of cucumber leaves was proposed. Mg deficient cucumber plants and Control plants were grown under non-soil conditions with special nutrient supply. Cucumber leaves were employed to collect hyperspectral images using a visible and near infrared (VIS/NIR) hyperspectral imaging system (400–900 nm) and determine reference chlorophyll concentrations using high performance liquid chromatography (HPLC). An optimal chlorophyll concentration calibration model (Rp = 0.9087) was constructed and used to detect chlorophyll distribution maps of Mg deficient leaves and Control leaves. Results shown that chlorophyll content distributed more unevenly on Mg deficient leaves than Control leaves. The Standard Deviation (SD) value of the chlorophyll content at all the pixels on a chlorophyll distribution map was calculated for Mg deficient diagnostics. An Mg deficiency diagnostics model with satisfied performance (diagnostic rate 93.33%) was obtained. The result indicated the SD value of chlorophyll concentrations on the whole cucumber leaf could be employed to diagnose Mg deficiency nondestructively.

[1]  F. Damatta,et al.  Magnesium decreases leaf scald symptoms on rice leaves and preserves their photosynthetic performance. , 2016, Plant physiology and biochemistry : PPB.

[2]  Di Wu,et al.  Potential of hyperspectral imaging combined with chemometric analysis for assessing and visualising tenderness distribution in raw farmed salmon fillets , 2014 .

[3]  Zou Xiaobo,et al.  Nondestructive diagnostics of nitrogen deficiency by cucumber leaf chlorophyll distribution map based on near infrared hyperspectral imaging , 2012 .

[4]  A. Moreira,et al.  Magnesium-manganese interaction in soybean cultivars with different nutritional requirements , 2017 .

[5]  N. Kirmani,et al.  Diagnosis and recommendation integrated system approach for major and micronutrient diagnostic norms for apple (Malus Domestica Borkh) under varying ages and management practices of apple orchards , 2017 .

[6]  J. Cayuela,et al.  Rapid Determination of Olive Oil Chlorophylls and Carotenoids by Using Visible Spectroscopy , 2014 .

[7]  L. Valdez-Aguilar,et al.  Biomass and Accumulation of Potassium, Calcium, and Magnesium in Gladiolus as Affected by Heat Units and Corm Size , 2018 .

[8]  N. Fageria,et al.  Efficiency of Magnesium Use by Common Bean Varieties Regarding Yield, Physiological Components, and Nutritional Status of Plants , 2015 .

[9]  B. Khaitov.  Effects of Rhizobium inoculation and magnesium application on growth and nodulation of soybean (Glycine max L.) , 2018, Journal of Plant Nutrition.

[10]  H. Kalaji,et al.  Structural and functional disorder in the photosynthetic apparatus of radish plants under magnesium deficiency. , 2018, Functional plant biology : FPB.

[11]  Yong He,et al.  Mapping of Chlorophyll and SPAD Distribution in Pepper Leaves During Leaf Senescence Using Visible and Near-Infrared Hyperspectral Imaging , 2016 .

[12]  Dongfeng Yang,et al.  Magnesium deficiency in plants: An urgent problem , 2016 .

[13]  I. Ortas.  Influence of potassium and magnesium fertilizer application on the yield and nutrient accumulation of maize genotypes under field conditions , 2018 .

[14]  M. Hojati,et al.  Magnesium and manganese affect photosynthesis, essential oil composition and phenolic compounds of Tanacetum parthenium. , 2017, Plant physiology and biochemistry : PPB.

[15]  Jiewen Zhao,et al.  A heuristic and parallel simulated annealing algorithm for variable selection in near‐infrared spectroscopy analysis , 2016 .

[16]  C. Abdelly,et al.  Effects of magnesium deficiency on photosynthesis and carbohydrate partitioning , 2016, Acta Physiologiae Plantarum.

[17]  M. Holmes,et al.  Near Infrared Quantitative Analysis of Total Flavonoid Content in Fresh Ginkgo Biloba Leaves Based on Different Wavelength Region Selection Methods and Partial Least Squares Regression , 2012 .

[18]  Oliver Schlüter,et al.  VIS/NIR spectroscopy, chlorophyll fluorescence, biospeckle and backscattering to evaluate changes in apples subjected to hydrostatic pressures , 2014 .

[19]  M. Souri,et al.  Effects of different magnesium levels on some morphophysiological characteristics and nutrient elements uptake in Khatouni melons (cucumis melo var. inodorus) , 2019, Journal of Plant Nutrition.

[20]  Jiewen Zhao,et al.  A rapid and nondestructive method to determine the distribution map of protein, carbohydrate and sialic acid on Edible bird's nest by hyper-spectral imaging and chemometrics. , 2017, Food chemistry.

[21]  E. A. El-Nour,et al.  Predicting nitrogen, magnesium and iron nutritional status in some perennial crops using a portable chlorophyll meter , 1999 .

[22]  F. Rodrigues,et al.  Effect of Magnesium on the Development of Sheath blight in Rice , 2014 .

[23]  Marian Wiwart,et al.  Early diagnostics of macronutrient deficiencies in three legume species by color image analysis , 2009 .

[24]  M. Holmes,et al.  Noise-free microbial colony counting method based on hyperspectral features of agar plates. , 2019, Food chemistry.

[25]  M. Župunski,et al.  Responses of Wheat (Triticum Aestivum L.) and Maize (Zea Mays L.) Plants to Cadmium Toxicity in Relation to Magnesium Nutrition , 2014 .

[26]  Jiyong Shi,et al.  Detection of triterpene acids distribution in loquat (Eriobotrya japonica) leaf using hyperspectral imaging. , 2018, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[27]  R. Schouten,et al.  Quantifying lycopene synthesis and chlorophyll breakdown in tomato fruit using remittance VIS spectroscopy , 2014 .