Human monocytes stimulation by particles of hydroxyapatite, silicon carbide and diamond: in vitro studies of new prosthesis coatings.

Aseptic loosening due to wear and debris formation constitutes the major problem in longevity of joint replacements. Diamond coated onto the prosthesis surface may reduce wear, owing to its excellent tribological properties. A thin diamond coating may be brittle, and we plan eventually to reinforce it with silicon carbide whiskers (SiC). In the present study we compared particles of diamond, SiC and hydroxyapatite (HA) in serum-free cultures of human monocytes. All particles were found to be phagocytozed, and monocyte morphology changed except after the ingestion of diamond. Interleukin-1 beta production was increased on average 30-fold and 38-fold in cultures exposed to HA and SiC, respectively, compared to control and diamond cultures (n = 6). Addition of the phagocytosis inhibitor cytochalasin B inhibited the morphological changes of the monocytes and reduced interleukin-1 beta production. In some experiments particles of polymethylmethacrylate were also included, and the interleukin-1 beta stimulation was in the same range as after HA and SiC stimulation. The results show that diamond particles in serum-free monocyte culture are inert, while SiC and HA have a stimulatory effect comparable to polymethylmethacrylate. With its excellent tribological and biocompatible properties, future studies with diamond coating are warranted.

[1]  A. Malcolm,et al.  The mechanism of loosening of cemented acetabular components in total hip arthroplasty. Analysis of specimens retrieved at autopsy. , 1992, Clinical orthopaedics and related research.

[2]  D. Baker,et al.  In vivo inflammatory response to polymethylmethacrylate particulate debris: Effect of size, morphology, and surface area , 1994, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[3]  C. Haslett,et al.  Phagocytosis of apoptotic neutrophils does not induce macrophage release of thromboxane B2 , 1992, Journal of leukocyte biology.

[4]  T. Hamilton,et al.  The cell biology of macrophage activation. , 1984, Annual review of immunology.

[5]  H. Oxlund,et al.  Local injection of TGF-β increases the strength of tibial fractures in the rat , 1994 .

[6]  P. Dieppe,et al.  A comparison of the effects of urate, hydroxyapatite and diamond crystals on polymorphonuclear cells: relationship of mediator release to the surface area and adsorptive capacity of different particles. , 1990, The Journal of rheumatology.

[7]  N. Athanasou,et al.  Polymethylmethacrylate-induced inflammatory macrophages resorb bone. , 1992, The Journal of bone and joint surgery. British volume.

[8]  C Baquey,et al.  Diamond: The Biomaterial of the 21st Century? , 1993, The International journal of artificial organs.

[9]  S. Goodman,et al.  The histological effects of the implantation of different sizes of polyethylene particles in the rabbit tibia. , 1990, Journal of biomedical materials research.

[10]  W. Maloney,et al.  Bone lysis in well-fixed cemented femoral components. , 1990, The Journal of bone and joint surgery. British volume.

[11]  R. Huiskes,et al.  Failed innovation in total hip replacement. Diagnosis and proposals for a cure. , 1993, Acta orthopaedica Scandinavica.

[12]  R Lappalainen,et al.  Benign response to particles of diamond and SiC: bone chamber studies of new joint replacement coating materials in rabbits. , 1996, Biomaterials.

[13]  J. Galante,et al.  Mechanisms of bone loss associated with total hip replacement. , 1993, The Orthopedic clinics of North America.

[14]  P. Aspenberg,et al.  Bone formation in the presence of phagocytosable hydroxyapatite particles. , 1994, Clinical orthopaedics and related research.

[15]  H. Haanaes,et al.  Alveolar ridge augmentation in rats by combined hydroxylapatite and osteoinductive material. , 1991, Scandinavian journal of dental research.

[16]  R. Bloebaum,et al.  Osteolysis from a press-fit hydroxyapatite-coated implant. A case study. , 1993, The Journal of arthroplasty.

[17]  N. Rushton,et al.  Biocompatibility of diamond-like carbon coating. , 1991, Biomaterials.

[18]  M. Semlitsch,et al.  Reactions of the articular capsule to wear products of artificial joint prostheses. , 1977, Journal of biomedical materials research.

[19]  D. Howie,et al.  The differences in toxicity and release of bone-resorbing mediators induced by titanium and cobalt-chromium-alloy wear particles. , 1993, The Journal of bone and joint surgery. American volume.

[20]  D. Howie,et al.  The response to particulate debris. , 1993, The Orthopedic clinics of North America.

[21]  M. W. Jones,et al.  Diamond-like carbon as biological compatible material for cell culture and medical application. , 1993, Bio-medical materials and engineering.

[22]  J. Galante,et al.  Macrophage/particle interactions: effect of size, composition and surface area. , 1994, Journal of biomedical materials research.

[23]  P. Delincé,et al.  Two-year outcome of hydroxyapatite-coated prostheses. Two femoral prostheses retrieved at autopsy. , 1994, Acta orthopaedica Scandinavica.

[24]  M. Freeman,et al.  Hydroxyapatite coating of hip prostheses. Effect on migration into the femur. , 1992, The Journal of bone and joint surgery. British volume.

[25]  C. Dinarello,et al.  The role of interleukin-1 in disease. , 1993, The New England journal of medicine.

[26]  S. Santoro,et al.  Bone matrix constituents stimulate interleukin-1 release from human blood mononuclear cells. , 1991, The Journal of clinical investigation.

[27]  E. Evans.  Toxicity of hydroxyapatite in vitro: the effect of particle size. , 1991, Biomaterials.

[28]  G. Mundy.  Role of cytokines in bone resorption , 1993, Journal of cellular biochemistry.

[29]  L. Dorr,et al.  Complications with hydroxyapatite particulate separation in total hip arthroplasty. , 1994, Clinical orthopaedics and related research.

[30]  A. Bøyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. , 1968 .

[31]  P. Revell,et al.  Interleukin-1 production by activated macrophages surrounding loosened orthopaedic implants: a potential role in osteolysis. , 1994, British journal of rheumatology.

[32]  J. Kärrholm,et al.  Early failure of hydroxyapatite-coating in total knee arthroplasty. A case report. , 1994, Acta orthopaedica Scandinavica.

[33]  J. H. Herman,et al.  Polymethylmethacrylate-induced release of bone-resorbing factors. , 1989, The Journal of bone and joint surgery. American volume.

[34]  P. Dieppe,et al.  Hydroxyapatite and urate crystal induced cytokine release by macrophages. , 1989, Annals of the rheumatic diseases.

[35]  W H Harris,et al.  Osteolysis and particle disease in hip replacement. A review. , 1994, Acta orthopaedica Scandinavica.

[36]  P. Delincé,et al.  Bonding of hydroxyapatite-coated femoral prostheses. Histopathology of specimens from four cases. , 1991, The Journal of bone and joint surgery. British volume.

[37]  C Baquey,et al.  Hemocompatibility of diamond-like carbon coating. , 1993, Bio-medical materials and engineering.

[38]  Collagen-induced release of interleukin 1 from human blood mononuclear cells. Potentiation by fibronectin binding to the alpha 5 beta 1 integrin. , 1992, The Journal of clinical investigation.

[39]  S. Goodman,et al.  Histological reaction to titanium alloy and hydroxyapatite particles in the rabbit tibia. , 1993, Biomaterials.