Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil

ABSTRACT A virus was isolated from soybean ( Glycine max ) plants with symptoms of dwarfing and bud blight in WenceslauBraz County, Parana, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purifiedvirus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related toTSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. TheCP gene shared 83% identity with the sequence of TSV CP from white clover ( Trifolium repens ) (GenBank CAA25133).This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed thatthis isolate be considered a strain of TSV named TSV-BR. Additional keywords : nucleotide sequence, Elisa, host range. RESUMOCaracterizacao biologica e molecular de um isolado de

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