SURVIVAL-CURVES OF SOLID TRANSPLANTABLE TUMOUR CELLS IRRADIATED IN VIVO: A METHOD OF DETERMINATION AND STATISTICAL EVALUATION; COMPARISON OF CELL-SURVIVAL AND 32-P-UPTAKE INTO DNA.

SummaryMethods for estimation of the 37 per cent dose (D37) of mammalian cells in vivo have depended on the use of neoplasms from which suspensions of single cells can be readily prepared. The method presented is based on the quantitative relationship between the volume of viable tumour cells in the transplant inoculum and the latency, i.e. the time elapsing from grafting until tumours are detectable. The procedure does not involve preparation of single-cell suspensions, and the data obtained are amenable to statistical evaluation.The in vivo D37 values for C3H/WR mouse adenocarcinoma strain MTG-B was 352 rads (95 per cent confidence limits : 304–401 rads) and for carcinoma 755, 319 rads (268–368 rads). The inhibition in the incorporation of radio-orthophosphate into the deoxyribonucleic acid of tumour strain MTG-B determined in one experiment did not reflect the degree of radiation-induced damage to the cell-population.

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