Novel and sensitive ELISA for the rapid quantification of recombinant p64K protein.

[1]  Julio C. Sánchez,et al.  A combinatorial strategy of a new monoclonal ELISA and immunoaffinity chromatography using sodium deoxycholate to increase the recovery of multimeric proteins like r-HBsAg. , 2010, Talanta.

[2]  A. Lage,et al.  Effective Inhibition of the Epidermal Growth Factor/Epidermal Growth Factor Receptor Binding by Anti–Epidermal Growth Factor Antibodies Is Related to Better Survival in Advanced Non–Small-Cell Lung Cancer Patients Treated with the Epidermal Growth Factor Cancer Vaccine , 2008, Clinical Cancer Research.

[3]  S. Morimoto,et al.  Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin. , 2008, Analytica chimica acta.

[4]  Antonio E Pérez,et al.  Safety and preliminary immunogenicity of MenC/P64k, a meningococcal serogroup C conjugate vaccine with a new recombinant carrier. , 2006, FEMS Immunology & Medical Microbiology.

[5]  G. Shankar,et al.  Validation of immunoassays used to assess immunogenicity to therapeutic monoclonal antibodies. , 2005, Journal of pharmaceutical and biomedical analysis.

[6]  R. Jankov,et al.  Development of Sandwich Enzyme‐Linked Immunosorbent Assay for Determination of Tetanus Toxoid Concentration , 2004, Journal of immunoassay & immunochemistry.

[7]  T. Fornstedt,et al.  Guidelines for analytical method development and validation of biotechnological synthesis of drugs. Production of a hydroxyprogesterone as model. , 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[8]  A. Musacchio,et al.  Production of a recombinant, immunogenic protein, P64k, of Neisseria meningitidis in Escherichia coli in fed-batch fermenters , 2002, Biotechnology Letters.

[9]  G. Chinea,et al.  Mapping of monoclonal antibodies specific to P64k: a common antigen of several isolates of Neisseria meningitidis. , 2001, Canadian journal of microbiology.

[10]  Vinod P. Shah,et al.  Bioanalytical Method Validation—A Revisit with a Decade of Progress , 2000, Pharmaceutical Research.

[11]  G. Chinea,et al.  B‐cell epitope mapping of the Neisseria meningitidis P64k protein using overlapping peptides , 2000, Biotechnology and applied biochemistry.

[12]  V. Besada,et al.  Expression in Escherichia coli of the lpdA gene, protein sequence analysis and immunological characterization of the P64k protein from Neisseria meningitidis , 1998, Biotechnology and applied biochemistry.

[13]  A. Lage,et al.  A novel cancer vaccine composed of human-recombinant epidermal growth factor linked to a carrier protein: report of a pilot clinical trial. , 1998, Annals of oncology : official journal of the European Society for Medical Oncology.

[14]  T. Prangé,et al.  Molecular structure of the lipoamide dehydrogenase domain of a surface antigen from Neisseria meningitidis. , 1997, Journal of molecular biology.

[15]  T. Zor,et al.  Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies. , 1996, Analytical biochemistry.

[16]  R. Bringas,et al.  A lipoamide dehydrogenase from Neisseria meningitidis has a lipoyl domain , 1995, Proteins.

[17]  M Haase,et al.  Stability testing on vaccines--results of activities of the International Conference on Harmonization (ICH) of technical requirements for registration of pharmaceuticals for human use. , 1994, Biologicals : journal of the International Association of Biological Standardization.

[18]  C. Jenkin,et al.  Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. , 1978, Immunochemistry.

[19]  P. Nakane,et al.  PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[20]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[21]  G. Chinea,et al.  A dengue-2 Envelope fragment inserted within the structure of the P64k meningococcal protein carrier enables a functional immune response against the virus in mice. , 2004, Journal of virological methods.

[22]  J. Vose.  High-dose chemotherapy and hematopoietic stem cell transplantation for relapsed or refractory diffuse large-cell non-Hodgkin's lymphoma. , 1998, Annals of oncology : official journal of the European Society for Medical Oncology.

[23]  W. G. Wood,et al.  "Matrix effects" in immunoassays. , 1991, Scandinavian journal of clinical and laboratory investigation. Supplementum.