Development of an ic-ELISA and colloidal gold strip for the detection of the beta-blocker carazolol

ABSTRACT A monoclonal antibody against carazolol (CRZ), 2F5, was generated for the construction of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip for detection of CRZ in milk and pig kidney samples. The antibody had a half-maximal inhibitory concentration of 0.29 ng/mL; its limit of detection was 0.068 ng/mL, and its linear range of detection was 0.137–0.638 ng/mL. For CRZ in milk samples and porcine kidney samples, the detection limits for this strip were 0.25 and 2.5 ng/mL, respectively. Analysis of CRZ in milk samples showed that the results obtained by immunochromatographic strip were consistent with those obtained by ic-ELISA. Therefore, the CG immunoassay is a sensitive screening method for semi-quantitative and qualitative detection of residual CRZ in milk and porcine kidney samples.

[1]  R. Pecori,et al.  Discovery of β-Adrenergic Receptors Blocker-Carbonic Anhydrase Inhibitor Hybrids for Multitargeted Antiglaucoma Therapy. , 2018, Journal of medicinal chemistry.

[2]  L. Ai,et al.  Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey , 2018 .

[3]  Liqiang Liu,et al.  Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs , 2018 .

[4]  Qin Li,et al.  A highly sensitive resonance Rayleigh scattering and colorimetric assay for the recognition of propranolol in β-adrenergic blocker. , 2017, Luminescence : the journal of biological and chemical luminescence.

[5]  Liqiang Liu,et al.  Preparation of an anti-dexamethasone monoclonal antibody and its use in development of a colloidal gold immunoassay , 2017 .

[6]  Zheng-Jun Xie,et al.  Development of an indirect competitive enzyme-linked immunosorbent assay and immunochromatographic assay for hydrocortisone residues in milk , 2017 .

[7]  Hua Kuang,et al.  Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the simultaneous detection of avermectin and ivermectin , 2017 .

[8]  Liqiang Liu,et al.  Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples , 2017 .

[9]  Liqiang Liu,et al.  Development of a highly sensitive ELISA and immunochromatographic strip to detect pentachlorophenol , 2016 .

[10]  Liqiang Liu,et al.  Development of an ELISA and Immunochromatographic Assay for Tetracycline, Oxytetracycline, and Chlortetracycline Residues in Milk and Honey Based on the Class-Specific Monoclonal Antibody , 2016, Food Analytical Methods.

[11]  Liqiang Liu,et al.  Development of an immunoassay for carbendazim based on a class-selective monoclonal antibody , 2015 .

[12]  R. He,et al.  High performance liquid chromatography coupled with resonance Rayleigh scattering for the detection of three fluoroquinolones and mechanism study. , 2015, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[13]  Zhiyong Tang,et al.  The Determination of Protein-Based Arsenic in Shrimp Tissues by Hydride Generation-Atomic Fluorescence Spectrometer , 2015, Food Analytical Methods.

[14]  Suxia Zhang,et al.  Simultaneous Determination of Three Tranquillizers in Lamb Liver by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry , 2015, Food Analytical Methods.

[15]  Changlong Hao,et al.  A highly sensitive enzyme-linked immunosorbent assay for copper(II) determination in drinking water , 2014 .

[16]  A. Abad‐Somovilla,et al.  Sensitive monoclonal antibody-based immunoassays for kresoxim-methyl analysis in QuEChERS-based food extracts. , 2014, Journal of agricultural and food chemistry.

[17]  Zhang Xiaomei,et al.  Determination of 9 β-blockers residues in dairy products by liquid chromatography-tandem mass spectrometry. , 2014 .

[18]  Limin He,et al.  Multiresidue analysis of nine β-agonists in animal muscles by LC-MS/MS based on a new polymer cartridge for sample cleanup. , 2013, Journal of separation science.

[19]  C. Stachel,et al.  Development and validation of a confirmatory method for the determination of tranquilisers and a β-blocker in porcine and bovine kidney by LC-MS/MS , 2013, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[20]  Limin He,et al.  Simultaneous Determination of Tranquilizers and Carazolol Residues in Swine Tissues by Liquid Chromatography-Tandem Mass Spectrometry , 2012 .

[21]  M. Hong,et al.  Simultaneous detection of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry. , 2012, Journal of agricultural and food chemistry.

[22]  J. Qiu,et al.  In vitro metabolic stability and metabolite profiling of TJ0711 hydrochloride, a newly developed vasodilatory β-blocker, using a liquid chromatography-tandem mass spectrometry method. , 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[23]  P. Haddad,et al.  Sweeping-micellar electrokinetic chromatography for the simultaneous analysis of tricyclic antidepressant and β-blocker drugs in wastewater. , 2011, Talanta.

[24]  T. Le,et al.  Rapid and sensitive enzyme-linked immunosorbent assay and immunochromatographic assay for the detection of chlortetracycline residues in edible animal tissues , 2011, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[25]  Cheng Cai,et al.  Determination of β-blockers in pharmaceutical and human urine by capillary electrophoresis with electrochemiluminescence detection and studies on the pharmacokinetics. , 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[26]  Qinghua He,et al.  Development of an immunochromatographic strip test for the rapid detection of deoxynivalenol in wheat and maize , 2010 .

[27]  W. Yu-xiang.  Development of A One-step Immunochromatographic Strip Test for Rapid Detection of Chlortetracycline Residue in Food , 2010 .

[28]  B. Shao,et al.  Simultaneous detection of residues of beta-adrenergic receptor blockers and sedatives in animal tissues by high-performance liquid chromatography/tandem mass spectrometry. , 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[29]  Yuqi Feng,et al.  Polymer monolith microextraction online coupled to hydrophilic interaction chromatography/mass spectrometry for analysis of beta2-agonist in human urine. , 2009, Journal of separation science.

[30]  A. Posyniak,et al.  Rapid method for the determination of tranquilizers and a beta-blocker in porcine and bovine kidney by liquid chromatography with tandem mass spectrometry. , 2009, Analytica chimica acta.

[31]  Liping Wang,et al.  Determination of four benzodiazepine residues in pork using multiwalled carbon nanotube solid-phase extraction and gas chromatography-mass spectrometry. , 2006, Journal of chromatography. A.

[32]  P. Delahaut,et al.  Multiresidue method for the detection of tranquillisers, xylazine, and a beta-blocker in animal production by liquid chromatography-tandem mass spectrometry. , 2004, Journal of chromatography. A.

[33]  C. Elliott,et al.  Development of a rapid screening test for veterinary sedatives and the beta-blocker carazolol in porcine kidney by ELISA. , 2004, The Analyst.