Analysis of microscopic images

Purpose – The purpose of this paper is to investigate technologies improving image quality and understanding in life‐science microscopy.Design/methodology/approach – The new technique of high‐content analysis is described, along with the equipment available from various manufacturers. Advances in fluorescence imaging and confocal microscopy are then addressed. The paper concludes by reporting a powerful 3D visualisation package, and equipment for networked viewing of high‐resolution microscopy images.Findings – High‐content analysis has developed rapidly in the last four or five years, due largely to improvements in the software interface. Automation and powerful software acquire and manage vast quantities of data, allowing scientists experiment afresh on archived images. Improvements in laser scanning techniques and the emergence of microLED arrays assist microscopy imaging of live cells, whilst techniques giving high‐spectral discrimination improve image understanding.Originality/value – The paper descr...