Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries
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George Georgiou | G. Georgiou | B. Iverson | Li Yi | M. Gebhard | Qing Li | Joseph M. Taft | Brent L Iverson | Qing Li | Li Yi | Mark C Gebhard | Joseph M Taft
[1] Q. Beg,et al. Bacterial alkaline proteases: molecular approaches and industrial applications , 2002, Applied Microbiology and Biotechnology.
[2] J. Kim,et al. Engineering of protease variants exhibiting altered substrate specificity. , 2005, Biochemical and biophysical research communications.
[3] Oliver Schilling,et al. Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites , 2008, Nature Biotechnology.
[4] D. Waugh. An overview of enzymatic reagents for the removal of affinity tags , 2011, Protein Expression and Purification.
[5] Jan C. Semenza,et al. ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway , 1990, Cell.
[6] C. Craik,et al. Papa's got a brand new tag: advances in identification of proteases and their substrates. , 2005, Trends in biotechnology.
[7] R C Stevens,et al. Design of high-throughput methods of protein production for structural biology. , 2000, Structure.
[8] P. Daugherty,et al. Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics , 2010, Biotechnology and bioengineering.
[9] Frances H. Arnold,et al. In the Light of Evolution III: Two Centuries of Darwin Sackler Colloquium: In the light of directed evolution: Pathways of adaptive protein evolution , 2009 .
[10] Christopher M. Overall,et al. In search of partners: linking extracellular proteases to substrates , 2007, Nature Reviews Molecular Cell Biology.
[11] Jasbir Singh,et al. Applications of microbial proteases in pharmaceutical industry: an overview , 2011 .
[12] W. Rutter,et al. Converting trypsin to chymotrypsin: the role of surface loops. , 1992, Science.
[13] P. Samuelson,et al. Substrate Profiling of Tobacco Etch Virus Protease Using a Novel Fluorescence-Assisted Whole-Cell Assay , 2011, PloS one.
[14] A. Wlodawer,et al. Structural Basis for the Substrate Specificity of Tobacco Etch Virus Protease* , 2002, The Journal of Biological Chemistry.
[15] Eric T. Boder,et al. Yeast surface display for screening combinatorial polypeptide libraries , 1997, Nature Biotechnology.
[16] Sami Mahrus,et al. Activation of Specific Apoptotic Caspases with an Engineered Small-Molecule-Activated Protease , 2010, Cell.
[17] J. Carrington,et al. Biochemical and mutational analysis of a plant virus polyprotein cleavage site. , 1988, The EMBO journal.
[18] George Georgiou,et al. Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. , 2005, Proceedings of the National Academy of Sciences of the United States of America.
[19] Dan S. Tawfik,et al. Directed evolution of the promiscuous esterase activity of carbonic anhydrase II. , 2005, Biochemistry.
[20] D. Rodgers,et al. Swapping the Substrate Specificities of the Neuropeptidases Neurolysin and Thimet Oligopeptidase* , 2007, Journal of Biological Chemistry.
[21] Tobias M. Fischer,et al. Monitoring regulated protein-protein interactions using split TEV , 2006, Nature Methods.
[22] Dan S. Tawfik,et al. High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. , 2005, Chemistry & biology.
[23] Proteases that can distinguish among different post-translational forms of tyrosine engineered using multicolor flow cytometry. , 2009, Journal of the American Chemical Society.
[24] S. Savinov,et al. Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System , 2012, Applied Biochemistry and Biotechnology.
[25] An engineered protease that cleaves specifically after sulfated tyrosine. , 2008, Angewandte Chemie.
[26] George Georgiou,et al. Directed Evolution of Highly Selective Proteases by Using a Novel FACS‐Based Screen that Capitalizes on the p53 Regulator MDM2 , 2012, Chembiochem : a European journal of chemical biology.
[27] Dan S. Tawfik,et al. Enzyme promiscuity: a mechanistic and evolutionary perspective. , 2010, Annual review of biochemistry.
[28] C. Craik,et al. Proteases as therapeutics. , 2011, The Biochemical journal.
[29] D. E. Anderson,et al. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. , 2001, Protein engineering.
[30] P. Daugherty. Protein engineering with bacterial display. , 2007, Current opinion in structural biology.
[31] I. Matsumura,et al. Diversification and specialization of HIV protease function during in vitro evolution. , 2006, Molecular biology and evolution.
[32] Critical Amino Acids in the Active Site of Meprin Metalloproteinases for Substrate and Peptide Bond Specificity* , 2003, Journal of Biological Chemistry.
[33] M. Pogson,et al. Engineering next generation proteases. , 2009, Current opinion in biotechnology.
[34] H. Pelham,et al. Sorting of soluble ER proteins in yeast. , 1988, The EMBO journal.
[35] George Georgiou,et al. Highly active and selective endopeptidases with programmed substrate specificities. , 2008, Nature chemical biology.
[36] D.,et al. Basic and Clinical Aspects of Fibrinolysis and Thrombolysis , 2003 .
[37] G. Salvesen,et al. Emerging principles in protease-based drug discovery , 2010, Nature Reviews Drug Discovery.