Nanotips for single-step preparation of DNA for qPCR analysis.

A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.

[1]  Chih-Ming Ho,et al.  Electrokinetic bioprocessor for concentrating cells and molecules. , 2004, Analytical chemistry.

[2]  M. Loriot,et al.  Collection of Human Genomic DNA From Buccal Cells for Genetics Studies: Comparison Between Cytobrush, Mouthwash, and Treated Card , 2005, Journal of biomedicine & biotechnology.

[3]  Woon-Hong Yeo,et al.  Size-specific concentration of DNA to a nanostructured tip using dielectrophoresis and capillary action. , 2009, The journal of physical chemistry. B.

[4]  P. Ke,et al.  Carbon nanomaterials in biological systems , 2007 .

[5]  Jerome P Ferrance,et al.  Chitosan as a polymer for pH-induced DNA capture in a totally aqueous system. , 2006, Analytical chemistry.

[6]  Steven A. Soper,et al.  Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips , 2006, Nucleic acids research.

[7]  Woon-Hong Yeo,et al.  Nanoscale sensor analysis using the immersed molecular electrokinetic finite element method. , 2012, Nanoscale.

[8]  M. R. Bown,et al.  AC electroosmotic flow in a DNA concentrator , 2006 .

[9]  Woon-Hong Yeo,et al.  Hybrid nanofibril assembly using an alternating current electric field and capillary action. , 2009, Journal of nanoscience and nanotechnology.

[10]  Woon-Hong Yeo,et al.  Immunosensor towards low-cost, rapid diagnosis of tuberculosis. , 2012, Lab on a chip.

[11]  Giorgia Antonelli,et al.  Saliva specimen: a new laboratory tool for diagnostic and basic investigation. , 2007, Clinica chimica acta; international journal of clinical chemistry.

[12]  Woon-Hong Yeo,et al.  Size-selective immunofluorescence of Mycobacterium tuberculosis cells by capillary- and viscous forces. , 2010, Lab on a chip.

[13]  M. Smits,et al.  Automated genomic DNA extraction from saliva using the QIAxtractor , 2010, Clinical Chemistry and Laboratory Medicine.

[14]  P. Burke,et al.  Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly. , 2004, Biosensors & bioelectronics.

[15]  Woon-Hong Yeo,et al.  Dielectrophoretic concentration of low-abundance nanoparticles using a nanostructured tip , 2012, Nanotechnology.

[16]  Woon-Hong Yeo,et al.  Rapid detection of Mycobacterium tuberculosis cells by using microtip-based immunoassay , 2009, Analytical and bioanalytical chemistry.

[17]  Marco Rito-Palomares,et al.  Dielectrophoresis for the manipulation of nanobioparticles , 2007, Electrophoresis.

[18]  M. Otte,et al.  Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology. , 2003, Legal medicine.