Quantification of Infarction in Cross Sections of Canine Myocardium In Vivo with Positron Emission Transaxial Tomography and 11C‐Palmitate

SUMMARYTo assess myocardial infarction quantitatively in 15 mm thick transverse sections of the canine heart in vivo we utilized a new technique, positron emission transaxial tomography (PETT) and cyclotron-produced 11C-palmitate (11C-P) injected intravenously. Results were compared to regional myocardial creatine phosphokinase (CPK) depletion, diminished 144C-palmitate accumulation in tissue extracts, and infarction estimated morphometrically 48 hours after coronary occlusion. CPK activity and 14C-P content declined in parallel In transmural biopsies (N = 44) from normal and ischemic zones (r = .92) in six dogs; and infarct in 10 mm thick cross sections of the entire left ventricle estimated morphometrically (N 26) in six other animals correlated with CPK depletion in contiguous 2.5 mm thick slices (r = .92). When the percentage of infarction in 15 mm thick cross sections was assessed tomographically in six other dogs 48 hours after coronary occlusion with 11C-P injected intravenously, results correlated with infarction in corresponding cross sections from the same hearts estimated morphometrically (r = .97, N = 9) and by analysis of CPK depletion (r = .93, N = 9). ThIls, PETT permits estimation of infarction in cross sections of the left ventricle In vivo after intravenous injection of 11C-palmitate.

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