Aldehyde Spiropyran Fluorescent Probe for Rapid Determination of Hydrazine in Environmental Water.

Hydrazine often receives publicity because it has a wide range of applications but high toxicity at the same time. Herein, we invented a novel aldehyde spiropyran fluorescent probe (SP-CHO) for fluorescent determination of hydrazine. The probe was synthesized through a hydralysis reaction and a condensation reaction. It exhibits specific response to hydrazine. The influence factors and anti-interference ability of SP-CHO identifying hydrazine were studied. HRMS, 1 H NMR and DFT calculations were used to reveal the recognition mechanism. Results showed that SP-CHO can be used for fluorescent determination of hydrazine with high selectivity and sensitivity. An SP-CHO-based fluorescent method was established for quantitation of hydrazine. The detection limit was 1.26 μmol/L, and the linear range is 5-100 μmol/L. The determination of hydrazine in water samples can be completed within 10 minutes, which shows good application prospects in real-time detection and process monitoring.