Proteome Analysis on Proteins Sequentially Extracted from Loach (Misgurnus anguillicaudatus)

Loach (Misgurnus anguillicaudatus) is known as a selenium-rich aquatic product. In present study, a sequential extraction method was applied for separation of water, salt and alkali-soluble proteins from loach (Misgurnus anguillicaudatus). The extracted proteins (included 12 water-soluble, 4 salt-soluble and 7 alkali-soluble proteins) were subjected to molecular weight analysis by SDS-PAGE and MALDI-TOF MS. Then, water soluble proteins were chosen for further ion exchange purification after selenium tracking by ICP-MS. The selenium containing peptides in water soluble proteins were identified by ESI-MS. Four proteins including creatine kinase, cytochrome P450 aromatase, beta-actin and glyceraldehyde 3-phosphate dehydrogenase were identified. Among these proteins, two selenium-containing peptides from beta-actin and cytochrome P450 aromatase were identified. The results of proteome analysis provide valuable molecular information of loach proteins. Moreover, it helps to understand the effect of selenium on redox systems in loach and its antioxidant functions.

[1]  K. Priyadarsini,et al.  Selenium nutrition: How important is it? , 2014 .

[2]  J. Landero-Figueroa,et al.  Identification of selenium-containing proteins in HEK 293 kidney cells using multiple chromatographies, LC-ICPMS and nano-LC-ESIMS. , 2013, Talanta.

[3]  Shiuh-Jen Jiang,et al.  Determination of selenium compounds in food supplements using reversed-phase liquid chromatography–inductively coupled plasma mass spectrometry , 2013 .

[4]  M. Kieliszek,et al.  Selenium: Significance, and outlook for supplementation. , 2013, Nutrition.

[5]  J. Loscalzo,et al.  Both selenium deficiency and modest selenium supplementation lead to myocardial fibrosis in mice via effects on redox-methylation balance. , 2012, Molecular nutrition & food research.

[6]  Dongbo Bu,et al.  MS-Simulator: predicting y-ion intensities for peptides with two charges based on the intensity ratio of neighboring ions. , 2012, Journal of proteome research.

[7]  S. Tanguy,et al.  Impact of dietary selenium intake on cardiac health: experimental approaches and human studies. , 2012, Molecular nutrition & food research.

[8]  Like Gong,et al.  Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC–ICP–MS and HPLC–ESI–MS/MS , 2012, European Food Research and Technology.

[9]  J. Caruso,et al.  Investigation of Se-containing proteins in Bertholletia excelsa H.B.K. (Brazil nuts) by ICPMS, MALDI-MS and LC–ESI-MS methods , 2011 .

[10]  Hong Zhang,et al.  ProbPS: A new model for peak selection based on quantifying the dependence of the existence of derivative peaks on primary ion intensity , 2011, BMC Bioinformatics.

[11]  Haifeng Zhao,et al.  Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates. , 2009 .

[12]  Y. Madrid,et al.  Novel approaches for selenium speciation in foodstuffs and biological specimens: a review. , 2009, Analytica chimica acta.

[13]  D. Arango,et al.  Expression of selenium-binding protein 1 characterizes intestinal cell maturation and predicts survival for patients with colorectal cancer. , 2008, Molecular nutrition & food research.

[14]  X. Lei,et al.  Knockouts of Se-glutathione peroxidase-1 and Cu,Zn superoxide dismutase exert different impacts on femoral mechanical performance of growing mice. , 2008, Molecular nutrition & food research.

[15]  M. Rayman.  Food-chain selenium and human health: emphasis on intake , 2008, British Journal of Nutrition.

[16]  M. Clavero,et al.  On the establishment and range expansion of oriental weatherfish (Misgurnus anguillicaudatus) in NE Iberian Peninsula , 2008, Biological Invasions.

[17]  U. Tinggi.  Selenium: its role as antioxidant in human health , 2008, Environmental health and preventive medicine.

[18]  H. Hansen,et al.  Complementary use of molecular and element-specific mass spectrometry for identification of selenium compounds related to human selenium metabolism , 2008, Analytical and bioanalytical chemistry.

[19]  M. Johansson,et al.  Glutathionylation of beta-actin via a cysteinyl sulfenic acid intermediary , 2007, BMC Biochemistry.

[20]  J. Szpunar,et al.  Selenopeptide mapping in a selenium-yeast protein digest by parallel nanoHPLC-ICP-MS and nanoHPLC-electrospray-MS/MS after on-line preconcentration , 2006 .

[21]  M. Mann,et al.  In-gel digestion for mass spectrometric characterization of proteins and proteomes , 2006, Nature Protocols.

[22]  Chenxiaoxue Zhang,et al.  Mechanism of apoptosis induced by a polysaccharide, from the loach Misgurnus anguillicaudatus (MAP) in human hepatocellular carcinoma cells. , 2006, Toxicology and Applied Pharmacology.

[23]  M. Bansal,et al.  Influence of selenium induced oxidative stress on spermatogenesis and lactate dehydrogenase-X in mice testis. , 2004, Asian journal of andrology.

[24]  M. Soriano-garcia.  Organoselenium compounds as potential therapeutic and chemopreventive agents: a review. , 2004, Current medicinal chemistry.

[25]  J. Szpunar,et al.  Methodological advances for selenium speciation analysis in yeast , 2003 .

[26]  W. Buchmann,et al.  Identification of water-soluble selenium-containing proteins in selenized yeast by size-exclusion-reversed-phase HPLC/ICPMS followed by MALDI-TOF and electrospray Q-TOF mass spectrometry. , 2003, Analytical chemistry.

[27]  J. Arthur,et al.  Selenium, selenoproteins and human health: a review , 2001, Public Health Nutrition.

[28]  W. Willett,et al.  Plasma levels of the antioxidant selenium and risk of myocardial infarction among U.S. physicians. , 1995, The American journal of cardiology.

[29]  J. Arthur.  Effects of selenium and vitamin E status on plasma creatine kinase activity in calves. , 1988, The Journal of nutrition.

[30]  A. Kappas,et al.  Selenium regulation of hepatic heme metabolism: induction of delta-aminolevulinate synthase and heme oxygenase. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[31]  H. Ganther,et al.  Selenium: Biochemical Role as a Component of Glutathione Peroxidase , 1973, Science.

[32]  Fuming Zhang,et al.  Selenium bioavailability from shrimps (Penaeus vannamei Boone) and its effect on the metabolism of phospholipid and cholesterol ester , 2014 .

[33]  J. Regenstein,et al.  In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion , 2011 .

[34]  J. Capelo,et al.  Enzymatic probe sonication: enhancement of protease-catalyzed hydrolysis of selenium bound to proteins in yeast. , 2004, Analytical chemistry.