Plasticity of marrow mesenchymal stem cells from human first-trimester fetus: from single-cell clone to neuronal differentiation.

Recent results have shown that bone marrow mesenchymal stem cells (BMSCs) from human first-trimester abortus (hfBMSCs) are closer to embryonic stem cells and perform greater telomerase activity and faster propagation than mid- and late-prophase fetal and adult BMSCs. However, no research has been done on the plasticity of hfBMSCs into neuronal cells using single-cell cloned strains without cell contamination. In this study, we isolated five single cells from hfBMSCs and obtained five single-cell cloned strains, and investigated their biological property and neuronal differentiation potential. We found that four of the five strains showed similar expression profile of surface antigen markers to hfBMSCs, and most of them differentiated into neuron-like cells expressing Nestin, Pax6, Sox1, β-III Tubulin, NF-L, and NSE under induction. One strain showed different expression profile of surface antigen markers from the four strains and hfBMSCs, and did not differentiate toward neuronal cells. We demonstrated for the first time that some of single-cell cloned strains from hfBMSCs can differentiate into nerve tissue-like cell clusters under induction in vitro, and that the plasticity of each single-cell cloned strain into neuronal cells is different.

[1]  Christian Clausen,et al.  Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. , 2003, Bone.

[2]  G. Kopen,et al.  MicroSAGE Analysis of 2,353 Expressed Genes in a Single Cell‐Derived Colony of Undifferentiated Human Mesenchymal Stem Cells Reveals mRNAs of Multiple Cell Lineages , 2001, Stem cells.

[3]  V. Silani,et al.  Neuro-glial differentiation of human bone marrow stem cells in vitro , 2005, Experimental Neurology.

[4]  Steven J. Greco,et al.  Neurons Derived From Human Mesenchymal Stem Cells Show Synaptic Transmission and Can Be Induced to Produce the Neurotransmitter Substance P by Interleukin‐1α , 2005, Stem cells.

[5]  Charles Tator,et al.  Bone marrow-derived mesenchymal stromal cells for the repair of central nervous system injury , 2007, Bone Marrow Transplantation.

[6]  M. Tuszynski,et al.  Induction of bone marrow stromal cells to neurons: Differentiation, transdifferentiation, or artifact? , 2004, Journal of neuroscience research.

[7]  L. Covarrubias,et al.  Basic fibroblast growth factor promotes epidermal growth factor responsiveness and survival of mesencephalic neural precursor cells. , 1999, Journal of neurobiology.

[8]  C. Svendsen,et al.  Requirement for Neurogenesis to Proceed through the Division of Neuronal Progenitors following Differentiation of Epidermal Growth Factor and Fibroblast Growth Factor‐2–Responsive Human Neural Stem Cells , 2004, Stem cells.

[9]  M. Rao,et al.  Transdifferentiation—fact or artifact , 2003, Journal of cellular biochemistry.

[10]  Matthias Schieker,et al.  Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer , 2008, Journal of cellular and molecular medicine.

[11]  M. Zurita,et al.  Bone marrow stromal cells for spinal cord repair: a challenge for contemporary neurobiology. , 2009, Histology and histopathology.

[12]  Vivian H. Fan,et al.  Epidermal Growth Factor as a Candidate for Ex Vivo Expansion of Bone Marrow–Derived Mesenchymal Stem Cells , 2006, Stem cells.

[13]  D. Hutmacher,et al.  Autocrine Fibroblast Growth Factor 2 Increases the Multipotentiality of Human Adipose‐Derived Mesenchymal Stem Cells , 2008, Stem cells.

[14]  J. Chan,et al.  Human First‐Trimester Fetal MSC Express Pluripotency Markers and Grow Faster and Have Longer Telomeres Than Adult MSC , 2007, Stem cells.

[15]  N. Fisk,et al.  Fetal stem cells: betwixt and between. , 2006, Seminars in reproductive medicine.

[16]  H. Meng,et al.  Differentiation of mesenchymal stem cells into dopaminergic neuron-like cells in vitro. , 2005, Biomedical and environmental sciences : BES.