Distinct infrared spectroscopic patterns of human basal cell carcinoma of the skin.

Infrared spectroscopy combined with high pressure (pressure-tuning infrared spectroscopy) was applied to the study of paired sections of basal cell carcinomas (BCC) and normal skin from ten patients. Atmospheric pressure IR spectra from BCC were dramatically different from those from the corresponding normal skin. Compared to their normal controls, BCCs displayed increased hydrogen bonding of the phosphodiester group of nucleic acids, decreased hydrogen bonding of the C--OH groups of proteins, increased intensity of the band at 972 cm-1, a decreased intensity ratio between the CH3 stretching and CH2 stretching bands, and accumulation of unidentified carbohydrates. Some of these changes are shared by all human epithelial malignancies studied to date, while some others appear as yet unique to basal cell carcinoma. The diagnostic value of infrared spectroscopy in BCC remains to be determined.

[1]  Stanley J. Miller,et al.  Biology of basal cell carcinoma (Part II). , 1991, Journal of the American Academy of Dermatology.

[2]  B. Rigas,et al.  Human colorectal cancers display abnormal Fourier-transform infrared spectra. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[3]  E. Papavassiliou,et al.  Phosphodiester Stretching Bands in the Infrared Spectra of Human Tissues and Cultured Cells , 1991 .

[4]  Basil Rigas,et al.  Infrared Spectra of Microtome Sections of Human Colon Tissues , 1990 .

[5]  Frank S. Parker,et al.  Applications of Infrared Spectroscopy in Biochemistry, Biology, and Medicine , 1971 .

[6]  Stanley J. Miller,et al.  Biology of basal cell carcinoma (Part I). , 1991, Journal of the American Academy of Dermatology.

[7]  D. Moffatt,et al.  Deconvolution, Derivation, and Smoothing of Spectra Using Fourier Transforms , 1984 .

[8]  B. Rigas,et al.  Human colon adenocarcinoma cell lines display infrared spectroscopic features of malignant colon tissues. , 1992, Cancer research.

[9]  B. Rigas,et al.  Infrared spectroscopy of exfoliated human cervical cells: evidence of extensive structural changes during carcinogenesis. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[10]  S. French,et al.  Distinctive infrared spectral features in liver tumor tissues of mice: evidence of structural modifications at the molecular level. , 1991, Experimental and molecular pathology.

[11]  J. M. Sanchez-Ruiz,et al.  A Fourier-transform infrared spectroscopic study of the phosphoserine residues in hen egg phosvitin and ovalbumin. , 1988, Biochemistry.