Polyphyletic origins of species of the anamorphic genus Geosmithia and the relationships of the cleistothecial genera: Evidence from 18S, 5S and 28S rDNA sequence analyses

The anamorphic genus Geosmithia, with the type species G. lavendula, includes species strictly lacking a teleomorph as well as species associated with the teleomorphs Talaromyces and Chromocleista. Our 18S rDNA sequence-based tree, inferred from 1586 alignable sites from 57 selected taxa within the Ascomycota and using two basidiomycetes as out- groups, clearly demonstrates that Geosmithia is a poly- phyletic taxon with evolutionary affinities to at least three groups of the euascomycete lineage within the

[1]  S. Osawa,et al.  Origin and evolution of organisms as deduced from 5S ribosomal RNA sequences. , 1987, Molecular biology and evolution.

[2]  Joost A. Stalpers,et al.  Ainsworth and Bisby's dictionary of the fungi: 9th edition. , 1995 .

[3]  J. Pitt.  The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces , 1981 .

[4]  K. Mullis,et al.  Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. , 1987, Methods in enzymology.

[5]  J. Pitt,et al.  Distribution of ubiquinones in Penicillium and related genera , 1991 .

[6]  M. Berbee.  Loculoascomycete origins and evolution of filamentous ascomycete morphology based on 18S rRNA gene sequence data. , 1996, Molecular biology and evolution.

[7]  S. Rehner,et al.  Molecular systematics of the Hypocreales: a teleomorph gene phylogeny and the status of their anamorphs , 1995 .

[8]  S. Landvik.  Neolecta, a fruit-body-producing genus of the basal ascomycetes, as shown by SSU and LSU rDNA sequences , 1996 .

[9]  J. W. Taylor,et al.  Convergence in ascospore discharge mechanism among pyrenomycete fungi based on 18S ribosomal RNA gene sequence. , 1992, Molecular phylogenetics and evolution.

[10]  H. Kuraishi.  Ubiquinone systems in fungi I. Distribution of ubiquinones in the major families of ascomycetes, basidiomycetes, and deuteromycetes, and their taxonomic implications , 1985 .

[11]  R. F. Cain,et al.  The Trichocomaceae (Ascomycetes): synonyms in recent publications , 1973 .

[12]  D. Malloch.  The Plectomycete Centrum , 1981 .

[13]  B. Efron,et al.  The Jackknife: The Bootstrap and Other Resampling Plans. , 1983 .

[14]  J. Pitt.  Species names in current use in the Trichocomaceae (Fungi, Eurotiales) , 1993 .

[15]  S. Rehner,et al.  Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences , 1994 .

[16]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[17]  D. Stevens,et al.  Ubiquinone systems of Coccidioides immitis, the causative agent of coccidioidomycosis. , 1993, FEMS microbiology letters.

[18]  R. Samson,et al.  Studies on Talaromyces and related genera I. Hamigera gen. nov. and Byssochlamys , 1971 .

[19]  J. Pitt,et al.  INTERFACES AMONG GENERA RELATED TO ASPERGILLUS AND PENICILLIUM , 1985 .

[20]  R. F. Cain,et al.  The Trichocomataceae: Ascomycetes with Aspergillus, Paecilomyces, and Penicillium imperfect states , 1972 .

[21]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[22]  J. W. Taylor,et al.  Phylogeny of Discomycetes and early radiations of the apothecial Ascomycotina inferred from SSU rDNA sequence data. , 1995, Experimental mycology.

[23]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[24]  G. Benny,et al.  A synopsis of the orders and families of Plectomycetes with keys to genera , 1980 .

[25]  R. Samson.  Paecilomyces and some allied hyphomycetes , 1974 .

[26]  M. Berbee,et al.  Dating the evolutionary radiations of the true fungi , 1993 .

[27]  H. Nishida,et al.  Archiascomycetes: detection of a major new lineage within the Ascomycota , 1994 .

[28]  J. I. Pitt,et al.  Phylogeny in the genus Penicillium: a morphologist's perspective , 1995 .

[29]  J. Pitt.  Geosmithia gen. nov. for Penicillium lavendulum and related species , 1979 .

[30]  M. Berbee,et al.  Molecular Evolution of Ascomycete Fungi: Phylogeny and Conflict , 1994 .

[31]  J. Sugiyama,et al.  Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8S and ITS ribosomal DNA sequence data. , 1995 .

[32]  T. White.  Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[33]  J. Sugiyama,et al.  Phylogenetic relationships among eleven selected species of Aspergillus and associated teleomorphic genera estimated from 18S ribosomal RNA partial sequences , 1991 .

[34]  J. Spatafora,et al.  MOLECULAR SYSTEMATICS OF UNITUNICATE PERITHECIAL ASCOMYCETES: THE CLAVICIPITALES-HYPOCREALES CONNECTION , 1993 .

[35]  J. W. Taylor,et al.  Two ascomycete classes based on fruiting-body characters and ribosomal DNA sequence. , 1992, Molecular biology and evolution.

[36]  K. Mullis,et al.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[37]  Steven B. Lee.  Isolation of DNA from fungal mycelia and single spores , 1990 .

[38]  R. Vilgalys,et al.  Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species , 1990, Journal of bacteriology.