Effect of Neutral and Acidic Oligosaccharides on Fecal IL-8 and Fecal Calprotectin in Preterm Infants

The gastrointestinal inflammatory response may play a role in the susceptibility of preterm infants for infections. We previously reported a trend toward lower endogenous infection morbidity after enteral supplementation of neutral and acidic oligosaccharides (SCGOS/LCFOS/AOS). We hypothesize that enteral supplementation of prebiotics may decrease infectious morbidity by reducing intestinal inflammation. Therefore, we aimed to determine the effect of enteral supplementation of prebiotics on intestinal inflammation, as measured by fecal IL-8 (f-IL-8) and calprotectin (f-calprotectin), in preterm infants. In a randomized controlled trial, infants with a GA <32 wk and/or birth weight <1,500 g received enteral supplementation of prebiotics or placebo (maltodextrin) between d 3 and 30 of life. F-IL-8 and f-calprotectin was assessed at baseline, d 7, 14, and 30 of life. In total, 113 infants were included. Baseline patient and nutritional characteristics were not different in the SCGOS/LCFOS/AOS (n = 55) and the placebo group (n = 58). Enteral supplementation of prebiotics had no effect on f-IL-8 and f-calprotectin. F-IL-8 and f-calprotectin were strongly correlated at all time points (p < 0.001). In conclusion, enteral supplementation of prebiotics (SCGOS/LCFOS/AOS) does not affect f-IL-8 and f-calprotectin levels in preterm infants.

[1]  J. Stockman.  Altered Fecal Microflora and Increased Fecal Calprotectin in Infants with Colic , 2011 .

[2]  W. Fetter,et al.  Neutral and acidic oligosaccharides in preterm infants: a randomized, double-blind, placebo-controlled trial. , 2010, The American journal of clinical nutrition.

[3]  R. Calabrese,et al.  High Faecal Calprotectin Levels in Healthy, Exclusively Breast-Fed Infants , 2009, Neonatology.

[4]  C. Lebrilla,et al.  Mass spectrometric methods for analysis of oligosaccharides in human milk. , 2009, Nutrition reviews.

[5]  V. Viallon,et al.  Fecal Calprotectin: Cutoff Values for Identifying Intestinal Distress in Preterm Infants , 2009, Journal of pediatric gastroenterology and nutrition.

[6]  W. Fetter,et al.  Design of a randomised controlled trial on immune effects of acidic and neutral oligosaccharides in the nutrition of preterm infants: carrot study , 2008, BMC pediatrics.

[7]  C. M. Cotten,et al.  Dynamic Change of Fecal Calprotectin in Very Low Birth Weight Infants during the First Month of Life , 2008, Neonatology.

[8]  G. Moro,et al.  Structural and functional aspects of prebiotics used in infant nutrition. , 2008, The Journal of nutrition.

[9]  J. Twisk,et al.  The analysis of randomised controlled trial data with more than one follow-up measurement. A comparison between different approaches , 2008, European Journal of Epidemiology.

[10]  R. Connor,et al.  Fecal calprotectin concentrations are higher in exclusively breastfed infants compared to those who are mixed-fed. , 2008, Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine.

[11]  H. Zali,et al.  Evaluation of growth inhibitory and apoptosis inducing activity of human calprotectin on the human gastric cell line (AGS). , 2008, Iranian biomedical journal.

[12]  M. Domellöf,et al.  Fecal Calprotectin in Very Low Birth Weight Infants , 2007, Journal of pediatric gastroenterology and nutrition.

[13]  N. Laforgia,et al.  Does calprotectin represent a regulatory factor in host defense or a drug target in inflammatory disease? , 2007, Endocrine, metabolic & immune disorders drug targets.

[14]  R. Baldassano,et al.  Lamina Propria and Circulating Interleukin-8 in Newly and Previously Diagnosed Pediatric Inflammatory Bowel Disease Patients , 2007, Digestive Diseases and Sciences.

[15]  L. Bode.  Recent advances on structure, metabolism, and function of human milk oligosaccharides. , 2006, The Journal of nutrition.

[16]  W. Fetter,et al.  The intestinal bacterial colonisation in preterm infants: a review of the literature. , 2006, Clinical nutrition.

[17]  L. Denson,et al.  Role of fecal calprotectin as a biomarker of intestinal inflammation in inflammatory bowel disease , 2006, Inflammatory bowel diseases.

[18]  J. Twisk,et al.  Applied analysis of recurrent events: a practical overview , 2005, Journal of Epidemiology and Community Health.

[19]  C. Field.  The immunological components of human milk and their effect on immune development in infants. , 2005, The Journal of nutrition.

[20]  A. Jaarsma,et al.  Activation of the Inflammatory Reaction within Minutes after Birth in Ventilated Preterm Lambs with Neonatal Respiratory Distress Syndrome , 2004, Neonatology.

[21]  L. Barbot,et al.  High faecal calprotectin concentrations in newborn infants , 2004, Archives of Disease in Childhood - Fetal and Neonatal Edition.

[22]  A. M. van den Neucker,et al.  Fecal calprotectin in healthy term and preterm infants. , 2004, Journal of pediatric gastroenterology and nutrition.

[23]  D. Carroll,et al.  Faecal calprotectin concentrations and diagnosis of necrotising enterocolitis , 2003, The Lancet.

[24]  A. Berstad,et al.  Faecal calprotectin levels in infants with infantile colic, healthy infants, children with inflammatory bowel disease, children with recurrent abdominal pain and healthy children , 2002, Acta paediatrica.

[25]  E. Claud,et al.  Hypothesis: inappropriate colonization of the premature intestine can cause neonatal necrotizing enterocolitis , 2001, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[26]  W. Walker,et al.  Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium. , 2001, The American journal of clinical nutrition.

[27]  W. Walker,et al.  Inflammation in the developing human intestine: A possible pathophysiologic contribution to necrotizing enterocolitis. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[28]  A. Berstad,et al.  Relationship between intestinal permeability and calprotectin concentration in gut lavage fluid. , 2000, Scandinavian journal of gastroenterology.

[29]  K. Mitsuyama,et al.  IL‐8 as an important chemoattractant for neutrophils in ulcerative colitis and Crohn's disease , 1994, Clinical and experimental immunology.

[30]  E. Lingaas,et al.  Antimicrobial actions of calcium binding leucocyte L1 protein, calprotectin , 1990, The Lancet.

[31]  J. Madara,et al.  Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers. , 1987, The Journal of clinical investigation.

[32]  M. J. Bell,et al.  Neonatal Necrotizing Enterocolitis: Therapeutic Decisions Based upon Clinical Staging , 1978, Annals of surgery.