Cell Walls and the Developmental Anatomy of the Brachypodium distachyon Stem Internode

While many aspects of plant cell wall polymer structure are known, their spatial and temporal distribution within the stem are not well understood. Here, we studied vascular system and fiber development, which has implication for both biofuel feedstock conversion efficiency and crop yield. The subject of this study, Brachypodium distachyon, has emerged as a grass model for food and energy crop research. Here, we conducted our investigation using B. distachyon by applying various histological approaches and Fourier transform infrared spectroscopy to the stem internode from three key developmental stages. While vascular bundle size and number did not change over time, the size of the interfascicular region increased dramatically, as did cell wall thickness. We also describe internal stem internode anatomy and demonstrate that lignin deposition continues after crystalline cellulose and xylan accumulation ceases. The vascular bundle anatomy of B. distachyon appears to be highly similar to domesticated grasses. While the arrangement of bundles within the stem is highly variable across grasses, B. distachyon appears to be a suitable model for the rind of large C4 grass crops. A better understanding of growth and various anatomical and cell wall features of B. distachyon will further our understanding of plant biomass accumulation processes.

[1]  M. L. Nelson,et al.  Relation of certain infrared bands to cellulose crystallinity and crystal lattice type. Part II. A new infrared ratio for estimation of crystallinity in celluloses I and II , 1964 .

[2]  Jones,et al.  Tracheary element differentiation uses a novel mechanism coordinating programmed cell death and secondary cell wall synthesis , 1999, Plant physiology.

[3]  J. Draper,et al.  Brachypodium distachyon. A new model system for functional genomics in grasses. , 2001, Plant physiology.

[4]  Q. Qian,et al.  BRITTLE CULM1, Which Encodes a COBRA-Like Protein, Affects the Mechanical Properties of Rice Plants Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.011775. , 2003, The Plant Cell Online.

[5]  D. Cosgrove.  Growth of the plant cell wall , 2005, Nature Reviews Molecular Cell Biology.

[6]  N. Carpita,et al.  Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles. , 1992, Plant physiology.

[7]  H. Fukuda,et al.  Direct evidence of active and rapid nuclear degradation triggered by vacuole rupture during programmed cell death in Zinnia. , 2001, Plant physiology.

[8]  C. Stewart.  Excretion and heartwood formation in living trees. , 1966, Science.

[9]  T. Umezawa,et al.  Progress of lignification mediated by intercellular transportation of monolignols during tracheary element differentiation of isolated Zinnia mesophyll cells. , 2001, Plant & cell physiology.

[10]  Chris Somerville,et al.  Cellulosic biofuels. , 2009, Annual review of plant biology.

[11]  Staffan Persson,et al.  Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[12]  L. Donaldson.  Lignification and lignin topochemistry - an ultrastructural view. , 2001, Phytochemistry.

[13]  Noureddine Abidi,et al.  Changes in the cell wall and cellulose content of developing cotton fibers investigated by FTIR spectroscopy. , 2014, Carbohydrate polymers.

[14]  J. Patrick.  Vascular System of the Stem of the Wheat Plant .II. Development , 1972 .

[15]  J. Doonan,et al.  Endosperm development in Brachypodium distachyon , 2010, Journal of experimental botany.

[16]  Chris Somerville,et al.  Cellulose synthesis in higher plants. , 2006, Annual review of cell and developmental biology.

[17]  R. Zhong,et al.  Vascular development in Arabidopsis. , 2002, International review of cytology.

[18]  Michael D. Abràmoff,et al.  Image processing with ImageJ , 2004 .

[19]  A. Barceló,et al.  Xylem parenchyma cells deliver the H2O2 necessary for lignification in differentiating xylem vessels , 2005, Planta.

[20]  P. Lancashire,et al.  A uniform decimal code for growth stages of crops and weeds , 1991 .

[21]  D. Bolam,et al.  Understanding the Biological Rationale for the Diversity of Cellulose-directed Carbohydrate-binding Modules in Prokaryotic Enzymes* , 2006, Journal of Biological Chemistry.

[22]  H. Tuominen,et al.  Xylem cell death: emerging understanding of regulation and function. , 2012, Journal of experimental botany.

[23]  G. Duxbury.  Fourier transform infrared spectroscopy , 1978, Nature.

[24]  A. Chesson,et al.  Characterisation of lignin from parenchyma and sclerenchyma cell walls of the maize internode , 1997 .

[25]  Seth Debolt,et al.  Relative Crystallinity of Plant Biomass: Studies on Assembly, Adaptation and Acclimation , 2008, PloS one.

[26]  Brian Wells,et al.  Neural Network Analyses of Infrared Spectra for Classifying Cell Wall Architectures1[W][OA] , 2007, Plant Physiology.

[27]  J. Knox,et al.  Monoclonal Antibodies to Plant Cell Wall Xylans and Arabinoxylans , 2005, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[28]  N. L. Owen,et al.  Infrared Studies of “Hard” and “Soft” Woods , 1989 .

[29]  Deborah L Sills,et al.  Using FTIR to predict saccharification from enzymatic hydrolysis of alkali‐pretreated biomasses , 2012, Biotechnology and bioengineering.

[30]  D. Mohnen.  Pectin structure and biosynthesis. , 2008, Current opinion in plant biology.

[31]  R. Marchessault.  Application of infra-red spectroscopy to cellulose and wood polysaccharides , 1962 .

[32]  E. Grotewold,et al.  Brachypodium as a Model for the Grasses: Today and the Future1[W] , 2011, Plant Physiology.

[33]  Zengyu Wang,et al.  Lignin deposition and associated changes in anatomy, enzyme activity, gene expression, and ruminal degradability in stems of tall fescue at different developmental stages. , 2002, Journal of agricultural and food chemistry.

[34]  H. Rogniaux,et al.  A comprehensive overview of grain development in Brachypodium distachyon variety Bd21 , 2011, Journal of experimental botany.

[35]  German Spangenberg,et al.  Functional Analyses of Caffeic Acid O-Methyltransferase and Cinnamoyl-CoA-Reductase Genes from Perennial Ryegrass (Lolium perenne)[W] , 2010, Plant Cell.

[36]  J. Vogel.  Unique aspects of the grass cell wall. , 2008, Current opinion in plant biology.

[37]  R. Hatfield,et al.  Isolates of cell types from sorghum stems: Digestion, cell wall and anatomical characteristics , 1993 .

[38]  I. Leitch,et al.  Nuclear DNA Amounts in Angiosperms , 1995 .

[39]  Royston Goodacre,et al.  Identification of Novel Genes in Arabidopsis Involved in Secondary Cell Wall Formation Using Expression Profiling and Reverse Genetics , 2005, The Plant Cell Online.

[40]  Olivier Leroux,et al.  Collenchyma: a versatile mechanical tissue with dynamic cell walls. , 2012, Annals of botany.

[41]  K. Esau.  Anatomy of seed plants , 1960 .

[42]  M. L. Nelson,et al.  Relation of certain infrared bands to cellulose crystallinity and crystal latticed type. Part I. Spectra of lattice types I, II, III and of amorphous cellulose , 1964 .

[43]  E. Kellogg,et al.  Evolutionary history of the grasses. , 2001, Plant physiology.

[44]  C. Chapple,et al.  The genetics of lignin biosynthesis: connecting genotype to phenotype. , 2010, Annual review of genetics.

[45]  Z. Ye.  Vascular tissue differentiation and pattern formation in plants. , 2002, Annual review of plant biology.

[46]  R. Sage,et al.  Photorespiration and the evolution of C4 photosynthesis. , 2012, Annual review of plant biology.

[47]  J. Patrick.  Vascular System of the Stem of the Wheat Plant. I. Mature State , 1972 .