Real time complete Stokes polarimetric imager based on a linear polarizer array camera for tissue polarimetric imaging

Tissue polarimetric imaging measures Mueller matrices of tissues or Stokes vectors of the emergent light from tissues (normally using incidence with a fixed polarization state) over a field of view, and has demonstrated utility in a number of surgical and diagnostic applications. Here we introduce a compact complete Stokes polarimetric imager that can work for multiple wavelength bands with a frame-rate suitable for real-time applications. The imager was validated with standard polarizing components, and then employed as a polarization state analyzer of a Mueller imaging polarimeter and a standalone Stokes imaging polarimeter respectively to image the process of dehydration of bovine tendon tissue. The results obtained in this work suggested that the polarization properties of the samples rich of collagen fibres can change with the degree of dehydration, and therefore, dehydration of the samples prepared for polarimetric imaging (e.g. polarimetric microscopy) should be carefully controlled.

[1]  Sanaz Alali,et al.  Rapid time-gated polarimetric Stokes imaging using photoelastic modulators. , 2013, Optics letters.

[2]  B. Drévillon,et al.  General and self-consistent method for the calibration of polarization modulators, polarimeters, and mueller-matrix ellipsometers. , 1999, Applied optics.

[3]  Claudio Iemmi,et al.  Optimization and performance criteria of a Stokes polarimeter based on two variable retarders. , 2010, Optics express.

[4]  Nirmalya Ghosh,et al.  Mueller matrix decomposition for extraction of individual polarization parameters from complex turbid media exhibiting multiple scattering, optical activity, and linear birefringence. , 2008, Journal of biomedical optics.

[5]  Nan Zeng,et al.  Characterizing the microstructures of biological tissues using Mueller matrix and transformed polarization parameters. , 2014, Biomedical optics express.

[6]  Kaushik Balakrishnan,et al.  Full-Stokes imaging polarimeter using an array of elliptical polarizer. , 2014, Optics express.

[7]  Ji Qi,et al.  A high definition Mueller polarimetric endoscope for tissue characterisation , 2016, Scientific reports.

[8]  Ji Qi,et al.  Mueller polarimetric imaging for surgical and diagnostic applications: a review , 2017, Journal of biophotonics.

[9]  Dwight C. Look,et al.  Optimum angles for a Mueller matrix polarimeter , 1994, Optics & Photonics.

[10]  Valery V Tuchin,et al.  Polarized light interaction with tissues , 2016, Journal of biomedical optics.

[11]  Sanaz Alali,et al.  Assessment of local structural disorders of the bladder wall in partial bladder outlet obstruction using polarized light imaging. , 2014, Biomedical optics express.

[12]  Hui Ma,et al.  Mapping local orientation of aligned fibrous scatterers for cancerous tissues using backscattering Mueller matrix imaging , 2014, Journal of biomedical optics.

[13]  Anthony Kim,et al.  Quantitative correlation between light depolarization and transport albedo of various porcine tissues. , 2012, Journal of biomedical optics.

[14]  SANAZ ALALI,et al.  Rapid wide-field Mueller matrix polarimetry imaging based on four photoelastic modulators with no moving parts. , 2016, Optics letters.

[15]  A. Bermak,et al.  Liquid-crystal micropolarimeter array for full Stokes polarization imaging in visible spectrum. , 2010, Optics express.

[16]  Paul J. Wu,et al.  Stokes polarimetry imaging of rat tail tissue in a turbid medium: degree of linear polarization image maps using incident linearly polarized light. , 2006, Journal of biomedical optics.

[17]  Quan Liu,et al.  Investigation on the potential of Mueller matrix imaging for digital staining , 2016, Journal of biophotonics.

[18]  Paul J. Wu,et al.  Stokes polarimetry imaging of rat‐tail tissue in a turbid medium using incident circularly polarized light , 2005, Lasers in surgery and medicine.

[19]  R. Chipman,et al.  Interpretation of Mueller matrices based on polar decomposition , 1996 .

[20]  James E. Millerd,et al.  Snap-shot imaging polarimeter: performance and applications , 2014, Sensing Technologies + Applications.

[21]  Kaushik Balakrishnan,et al.  Polarization microscope using a near infrared full-Stokes imaging polarimeter. , 2015, Optics express.

[22]  Yu. A. Ushenko,et al.  Mueller-matrix mapping of optically anisotropic fluorophores of biological tissues in the diagnosis of cancer , 2014 .

[23]  Danail Stoyanov,et al.  Polarised stereo endoscope and narrowband detection for minimal access surgery. , 2014, Biomedical optics express.

[24]  Lihong V. Wang,et al.  Propagation of polarized light in birefringent turbid media: a Monte Carlo study. , 2002, Journal of biomedical optics.

[25]  E. García-Caurel,et al.  General methods for optimized design and calibration of Mueller polarimeters , 2004 .

[26]  Christopher G. Rylander,et al.  Dehydration mechanism of optical clearing in tissue. , 2006, Journal of biomedical optics.

[27]  D. Elson,et al.  Polarized multispectral imaging in a rigid endoscope based on elastic light scattering spectroscopy , 2012, Biomedical optics express.

[28]  Hui Ma,et al.  Quantitatively characterizing the microstructural features of breast ductal carcinoma tissues in different progression stages by Mueller matrix microscope. , 2017, Biomedical optics express.

[29]  Qingming Luo,et al.  Recent progress in tissue optical clearing , 2013, Laser & photonics reviews.

[30]  David A. Jaffray,et al.  Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues , 2015, Chemical science.

[31]  Igor Meglinski,et al.  Application of circularly polarized light for non‐invasive diagnosis of cancerous tissues and turbid tissue‐like scattering media , 2015, Journal of biophotonics.

[32]  Ye Wang,et al.  Mueller matrix microscope: a quantitative tool to facilitate detections and fibrosis scorings of liver cirrhosis and cancer tissues , 2016, Journal of biomedical optics.

[33]  Angelo Pierangelo,et al.  Polarimetric imaging of uterine cervix: a case study. , 2013, Optics express.

[34]  Y. Ushenko,et al.  Investigation of formation and interrelations of polarization singular structure and Mueller-matrix images of biological tissues and diagnostics of their cancer changes. , 2011, Journal of biomedical optics.

[35]  Hamid Ali,et al.  Ex Vivo Assessment of Carbon Tetrachloride (CCl4)-Induced Chronic Injury Using Polarized Light Spectroscopy , 2013, Applied spectroscopy.

[36]  A. Pierangelo,et al.  Ex-vivo characterization of human colon cancer by Mueller polarimetric imaging. , 2011, Optics express.