[24] Nitric oxide assay using hemoglobin method

Publisher Summary Hemoglobin, along with related hemoproteins such as myoglobin, can be used for the measurement of nitric oxide (NO). The technique is based on the direct reaction between NO and the oxygenated, ferrous form of hemoglobin (HbO 2 ), which yields the ferric form, methemoglobin (metHb), and nitrate. This technique has the following advantages over other methods of NO measurement: all reagents are inexpensive and readily available, no complex techniques are required for sample collection or handling, the only large equipment needed for the basic technique is a spectrophotometer, and the assay is conducted under physiological conditions. This chapter discusses the basic method and describes the applications of the method to a variety of model systems, and points out possible interferences and artifacts. The chapter discusses the reaction that is the basis for the use of hemoproteins as efficient agents to block the biological effects of NO (the endothelium-derived relaxing factor, EDRF). This reaction is also proposed as a basis for the measurement of NO, using electron spin resonance (ESR) to detect and quantify the nitrosylhemoprotein product.

[1]  C. E. Castro,et al.  Conversion of oxyhemoglobin to methemoglobin by organic and inorganic reductants. , 1978, Biochemistry.

[2]  L. Hagler,et al.  Metmyoglobin reductase. Identification and purification of a reduced nicotinamide adenine dinucleotide-dependent enzyme from bovine heart which reduces metmyoglobin. , 1979, The Journal of biological chemistry.

[3]  H. Sies,et al.  Nitric oxide production by cultured aortic endothelial cells in response to thiol depletion and replenishment. , 1991, The Journal of biological chemistry.

[4]  M. Feelisch,et al.  Correlation between nitric oxide formation during degradation of organic nitrates and activation of guanylate cyclase. , 1987, European journal of pharmacology.

[5]  T. Hollocher,et al.  Trapping of nitric oxide produced during denitrification by extracellular hemoglobin. , 1988, The Journal of biological chemistry.

[6]  P. O'Brien,et al.  Reductive metabolism of nitroprusside in rat hepatocytes and human erythrocytes. , 1991, Archives of biochemistry and biophysics.

[7]  Å. Wennmalm,et al.  Detection of endothelial-derived relaxing factor in human plasma in the basal state and following ischemia using electron paramagnetic resonance spectrometry. , 1990, Analytical biochemistry.

[8]  K. Imaizumi,et al.  A kinetic study on functional impairment of nitric oxide-exposed rat erythrocytes. , 1987, Environmental health perspectives.

[9]  J. Deleo,et al.  Nitric oxide hemoglobin in mice and rats in endotoxic shock. , 1991, Life sciences.

[10]  R. Furchgott,et al.  Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta. , 1985, The Journal of pharmacology and experimental therapeutics.

[11]  H. Oda,et al.  Nitrosyl-hemoglobin formation in the blood of animals exposed to nitric oxide. , 1975, Archives of environmental health.

[12]  F. Echlin.  Spasm of basilar and vertebral arteries caused by experimental subarachnoid hemorrhage. , 1965, Journal of neurosurgery.

[13]  R. Johns,et al.  Comparison of spectrophotometric and biological assays for nitric oxide (NO) and endothelium-derived relaxing factor (EDRF): nonspecificity of the diazotization reaction for NO and failure to detect EDRF. , 1990, The Journal of pharmacology and experimental therapeutics.

[14]  R. Smith,et al.  Nitroprusside produces cyanide poisoning via reaction with hemoglobin. , 1974, The Journal of pharmacology and experimental therapeutics.

[15]  J. Schrader,et al.  Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells. , 1988, Biochemical and biophysical research communications.

[16]  C. Nathan,et al.  N omega-hydroxy-L-arginine is an intermediate in the biosynthesis of nitric oxide from L-arginine. , 1991, The Journal of biological chemistry.

[17]  R. Smith,et al.  Nitric oxide hemoglobin in patients receiving nitroglycerin as detected by electron paramagnetic resonance spectroscopy. , 1992, The Journal of laboratory and clinical medicine.

[18]  L. Heppel,et al.  Metabolism of inorganic nitrite and nitrate esters; the coupled oxidation of nitrite by peroxide-forming systems and catalase. , 1949, The Journal of biological chemistry.

[19]  Å. Wennmalm,et al.  Dependence of the metabolism of nitric oxide (NO) in healthy human whole blood on the oxygenation of its red cell haemoglobin , 1992, British journal of pharmacology.

[20]  K. Shikama,et al.  Oxidation of oxymyoglobin to metmyoglobin with hydrogen peroxide: involvement of ferryl intermediate. , 1987, Biochemistry.

[21]  J. Herman,et al.  Autocatalytic oxidation of hemoglobin induced by nitrite: activation and chemical inhibition. , 1985, Journal of free radicals in biology & medicine.

[22]  H. Mizukami,et al.  Reaction of nitric oxide with heme proteins and model compounds of hemoglobin. , 1987, Biochemistry.

[23]  M. Feelisch,et al.  Nitric oxide (NO) formation from nitrovasodilators occurs independently of hemoglobin or non-heme iron. , 1987, European journal of pharmacology.

[24]  H. Mota-Filipe,et al.  Effect of DL-propranolol on nitric oxide production in perfused rat hindquarters. , 1992, European journal of pharmacology.

[25]  J. Kanner,et al.  Nitric oxide as an antioxidant. , 1991, Archives of biochemistry and biophysics.

[26]  M. Doyle,et al.  Oxidation of nitrogen oxides by bound dioxygen in hemoproteins. , 1981, Journal of inorganic biochemistry.

[27]  R. Smith,et al.  Red blood cells generate nitric oxide from directly acting, nitrogenous vasodilators. , 1987, Toxicology and applied pharmacology.

[28]  M. Doyle,et al.  Structural effects in alkyl nitrite oxidation of human hemoglobin. , 1984, The Journal of biological chemistry.

[29]  D. Bazylinski,et al.  Metmyoglobin and methemoglobin as efficient traps for nitrosyl hydride (nitroxyl) in neutral aqueous solution , 1985 .

[30]  J. Schrader,et al.  Nitric oxide release from the isolated guinea pig heart. , 1988, European journal of pharmacology.

[31]  C. Winterbourn.  Free-radical production and oxidative reactions of hemoglobin. , 1985, Environmental health perspectives.

[32]  G. Gans,et al.  Concomaitant release of nitric oxide and generation of Superoxide during the non-enzymatic decay of SIN-1 , 1990 .

[33]  P. B. Roberts,et al.  The rate of reaction of superoxide radical ion with oxyhaemoglobin and methaemoglobin. , 1976, The Biochemical journal.

[34]  G. Kojda,et al.  Spectrophotometric determination of nitric oxide using hemoglobin , 1992 .