A chromatin remodelling complex involved in transcription and DNA processing

The packaging of the eukaryotic genome in chromatin presents barriers that restrict the access of enzymes that process DNA. To overcome these barriers, cells possess a number of multi-protein, ATP-dependent chromatin remodelling complexes, each containing an ATPase subunit from the SNF2/SWI2 superfamily. Chromatin remodelling complexes function by increasing nucleosome mobility and are clearly implicated in transcription. Here we have analysed SNF2/SWI2- and ISWI-related proteins to identify remodelling complexes that potentially assist other DNA transactions. We purified a complex from Saccharomyces cerevisiae that contains the Ino80 ATPase. The INO80 complex contains about 12 polypeptides including two proteins related to the bacterial RuvB DNA helicase, which catalyses branch migration of Holliday junctions. The purified complex remodels chromatin, facilitates transcription in vitro and displays 3′ to 5′ DNA helicase activity. Mutants of ino80 show hypersensitivity to agents that cause DNA damage, in addition to defects in transcription. These results indicate that chromatin remodelling driven by the Ino80 ATPase may be connected to transcription as well as DNA damage repair.

[1]  J. Palmer,et al.  Characterization of the imitation switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. , 1999, Genes & development.

[2]  Y. Kurokawa,et al.  TIP49b, a New RuvB-like DNA Helicase, Is Included in a Complex Together with Another RuvB-like DNA Helicase, TIP49a* , 1999, The Journal of Biological Chemistry.

[3]  D. Stillman,et al.  Multiple links between the NuA4 histone acetyltransferase complex and epigenetic control of transcription. , 2000, Molecular cell.

[4]  Keji Zhao,et al.  Rapid and Phosphoinositol-Dependent Binding of the SWI/SNF-like BAF Complex to Chromatin after T Lymphocyte Receptor Signaling , 1998, Cell.

[5]  B. Emerson,et al.  Transcription of chromatin: these are complex times. , 1998, Current opinion in genetics & development.

[6]  R. Tjian,et al.  TAFs and TFIIA mediate differential utilization of the tandem Adh promoters , 1995, Cell.

[7]  R. Kornberg,et al.  Twenty-Five Years of the Nucleosome, Fundamental Particle of the Eukaryote Chromosome , 1999, Cell.

[8]  Y. L. Lin,et al.  An Eukaryotic RuvB-like Protein (RUVBL1) Essential for Growth* , 1998, The Journal of Biological Chemistry.

[9]  A. Birkmann,et al.  The product of the SNF2/SWI2 paralogue INO80 of Saccharomyces cerevisiae required for efficient expression of various yeast structural genes is part of a high‐molecular‐weight protein complex , 1999, Molecular microbiology.

[10]  Y. Seo,et al.  A DNA Helicase from Schizosaccharomyces pombeStimulated by Single-stranded DNA-binding Protein at Low ATP Concentration* , 1997, The Journal of Biological Chemistry.

[11]  B. Cairns,et al.  Two Actin-Related Proteins Are Shared Functional , 1998 .

[12]  S. Ehrlich,et al.  RuvAB Acts at Arrested Replication Forks , 1998, Cell.

[13]  T. Richmond,et al.  DNA binding within the nucleosome core. , 1998, Current opinion in structural biology.

[14]  M. Muramatsu,et al.  Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB. , 1997, Biochemical and biophysical research communications.

[15]  L. Hartwell,et al.  Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage , 1990, Molecular and cellular biology.

[16]  J. T. Kadonaga.  Eukaryotic Transcription: An Interlaced Network of Transcription Factors and Chromatin-Modifying Machines , 1998, Cell.

[17]  M. Cole,et al.  An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. , 2000, Molecular cell.

[18]  P. Hanawalt,et al.  Title Evolution of the SNF 2 family of proteins : Subfamilies with distinct sequences and functions Permalink , 2022 .

[19]  J. Workman,et al.  Alteration of nucleosome structure as a mechanism of transcriptional regulation. , 1998, Annual review of biochemistry.

[20]  S. West,et al.  Processing of recombination intermediates by the RuvABC proteins. , 1997, Annual review of genetics.

[21]  Paul Tempst,et al.  RSC, an Essential, Abundant Chromatin-Remodeling Complex , 1996, Cell.

[22]  G. Mizuguchi,et al.  Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin. , 1997, Molecular cell.

[23]  C. Peterson.  Multiple SWItches to turn on chromatin? , 1996, Current opinion in genetics & development.

[24]  R. Sandaltzopoulos,et al.  Inorganic pyrophosphatase is a component of the Drosophila nucleosome remodeling factor complex. , 1998, Genes & development.

[25]  Toshio Tsukiyama,et al.  ISWI, a member of the SWl2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor , 1995, Cell.

[26]  J. Workman,et al.  Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. , 1994, Science.

[27]  Jun Qin,et al.  Involvement of the TIP60 Histone Acetylase Complex in DNA Repair and Apoptosis , 2000, Cell.

[28]  S. Elledge,et al.  Identification of RNR4, encoding a second essential small subunit of ribonucleotide reductase in Saccharomyces cerevisiae , 1997, Molecular and cellular biology.

[29]  Carl Wu,et al.  Purification and properties of an ATP-dependent nucleosome remodeling factor , 1995, Cell.

[30]  J. Haber.  DNA recombination: the replication connection. , 1999, Trends in biochemical sciences.