Ratiometric fluorescent probe for sensing Streptococcus mutans glucosyltransferase, a key factor in the formation of dental caries.

We report on a naphthalimide ratiometric fluorescent probe for the real-time sensing and imaging of pathogenic bacterial glucosyltransferases, which are associated with the development of dental caries. Using a high-throughput screening method, we identified that several natural polyphenols from green tea were GTFs inhibitors that could eventually lead to suitable oral treatments to prevent the development of dental caries.

[1]  Xiaochi Ma,et al.  A two-photon ratiometric fluorescent probe for imaging and quantitative analysis of botanic glucosyltransferase: A key enzyme for the biosynthesis of bioactive glycosides , 2019, Sensors and Actuators B: Chemical.

[2]  H. Tian,et al.  Fluorogenic probes for disease-relevant enzymes. , 2019, Chemical Society reviews.

[3]  T. James,et al.  Molecular Design Strategy to Construct the Near-Infrared Fluorescent Probe for Selectively Sensing Human Cytochrome P450 2J2. , 2018, Journal of the American Chemical Society.

[4]  Saran Long,et al.  In situ imaging of aminopeptidase N activity in hepatocellular carcinoma: a migration model for tumour using an activatable two-photon NIR fluorescent probe , 2018, Chemical science.

[5]  Xiaochi Ma,et al.  Identification and Isolation of Glucosytransferases (GT) Expressed Fungi Using a Two-Photon Ratiometric Fluorescent Probe Activated by GT. , 2018, Analytical chemistry.

[6]  W. Tan,et al.  Recent progresses in small-molecule enzymatic fluorescent probes for cancer imaging. , 2018, Chemical Society reviews.

[7]  Xiaochi Ma,et al.  Highly Selective NIR Probe for Intestinal β-Glucuronidase and High-Throughput Screening Inhibitors to Therapy Intestinal Damage. , 2018, ACS sensors.

[8]  Xiaochi Ma,et al.  Isolation of γ-Glutamyl-Transferase Rich-Bacteria from Mouse Gut by a Near-Infrared Fluorescent Probe with Large Stokes Shift. , 2018, Analytical chemistry.

[9]  Xiaochi Ma,et al.  A highly selective ratiometric fluorescent probe for real-time imaging of β-glucuronidase in living cells and zebrafish , 2018, Sensors and Actuators B: Chemical.

[10]  Xiaochi Ma,et al.  Activatable Near-Infrared Fluorescent Probe for Dipeptidyl Peptidase IV and Its Bioimaging Applications in Living Cells and Animals. , 2018, Analytical chemistry.

[11]  Xiaochi Ma,et al.  Highly Specific near-Infrared Fluorescent Probe for the Real-Time Detection of β-Glucuronidase in Various Living Cells and Animals. , 2018, Analytical chemistry.

[12]  Guangbo Ge,et al.  A Practical and High-Affinity Fluorescent Probe for Uridine Diphosphate Glucuronosyltransferase 1A1: A Good Surrogate for Bilirubin. , 2017, Journal of medicinal chemistry.

[13]  Guoqiang Feng,et al.  A colorimetric and ratiometric fluorescent probe with enhanced near-infrared fluorescence for selective detection of cysteine and its application in living cells , 2017 .

[14]  S. Park,et al.  A carboxylesterase-selective ratiometric fluorescent two-photon probe and its application to hepatocytes and liver tissues , 2016, Chemical science.

[15]  Xiaohua Li,et al.  Sensitive and Selective Ratiometric Fluorescence Probes for Detection of Intracellular Endogenous Monoamine Oxidase A. , 2016, Analytical chemistry.

[16]  Xuedong Zhou,et al.  Molecule Targeting Glucosyltransferase Inhibits Streptococcus mutans Biofilm Formation and Virulence , 2015, Antimicrobial Agents and Chemotherapy.

[17]  Fang Zeng,et al.  Ratiometric fluorescence assay for γ-glutamyltranspeptidase detection based on a single fluorophore via analyte-induced variation of substitution. , 2014, Chemical communications.

[18]  Kan Saito,et al.  Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans , 2011, Clinical and Vaccine Immunology.

[19]  Xiaoling Zhang,et al.  A 4-hydroxynaphthalimide-derived ratiometric fluorescent chemodosimeter for imaging palladium in living cells. , 2011, Chemical communications.

[20]  S. Iwata,et al.  Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans. , 2011, Journal of molecular biology.

[21]  T. Gunnlaugsson,et al.  Colorimetric and fluorescent anion sensors: an overview of recent developments in the use of 1,8-naphthalimide-based chemosensors. , 2010, Chemical Society reviews.

[22]  W. Bowen,et al.  Interactions of Streptococcus mutans Glucosyltransferase B with Lysozyme in Solution and on the Surface of Hydroxyapatite , 2005, Caries Research.

[23]  A. Yanagida,et al.  Purification of glucosyltransferase from cell-lysate of Streptococcus mutans by counter-current chromatography using aqueous polymer two-phase system. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[24]  Hao Wu,et al.  Correction: A versatile two-photon fluorescent probe for ratiometric imaging E. coliβ-galactosidase in live cells and in vivo. , 2016, Chemical communications.