Application of EMA-qPCR as a complementary tool for the detection and monitoring of Legionella in different water systems

[1]  A. Nocker,et al.  Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. , 2011, Journal of Microbiological Methods.

[2]  C. Chang,et al.  Rapid quantification of viable legionellae in water and biofilm using ethidium monoazide coupled with real‐time quantitative PCR , 2010, Journal of applied microbiology.

[3]  P. Delgado-Viscogliosi,et al.  Viability PCR, a Culture-Independent Method for Rapid and Selective Quantification of Viable Legionella pneumophila Cells in Environmental Water Samples , 2009, Applied and Environmental Microbiology.

[4]  Haruo Watanabe,et al.  Specific Detection of Viable Legionella Cells by Combined Use of Photoactivated Ethidium Monoazide and PCR/Real-Time PCR , 2008, Applied and Environmental Microbiology.

[5]  B. Pozzetto,et al.  Use of Flow Cytometry To Monitor Legionella Viability , 2008, Applied and Environmental Microbiology.

[6]  J. Frére,et al.  Long-Term Survival of Legionella pneumophila in the Viable But Nonculturable State After Monochloramine Treatment , 2008, Current Microbiology.

[7]  R. Witthuhn,et al.  Selective PCR detection of viable Enterobacter sakazakii cells utilizing propidium monoazide or ethidium bromide monoazide , 2008, Journal of applied microbiology.

[8]  R. Berkelman,et al.  Increasing incidence of legionellosis in the United States, 1990-2005: changing epidemiologic trends. , 2008, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[9]  S. Soda,et al.  Application of real-time polymerase chain reaction (PCR) coupled with ethidium monoazide treatment for selective quantification of viable bacteria in aquatic environment. , 2008, Water science and technology : a journal of the International Association on Water Pollution Research.

[10]  N. Caroff,et al.  Real-time PCR assay for the detection and quantification of Legionella pneumophila in environmental water samples: utility for daily practice. , 2008, International journal of hygiene and environmental health.

[11]  V. Pellizari,et al.  Occurrence and Diversity of Legionellaceae in Polar Lakes of the Antarctic Peninsula , 2008, Current Microbiology.

[12]  F. Vandenesch,et al.  A PCR-Based Method for Monitoring Legionella pneumophila in Water Samples Detects Viable but Noncultivable Legionellae That Can Recover Their Cultivability , 2008, Applied and Environmental Microbiology.

[13]  S. Berk,et al.  Packaging of Live Legionella pneumophila into Pellets Expelled by Tetrahymena spp. Does Not Require Bacterial Replication and Depends on a Dot/Icm-Mediated Survival Mechanism , 2008, Applied and Environmental Microbiology.

[14]  F. Breidt,et al.  Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells , 2007, Applied and Environmental Microbiology.

[15]  C. Chang,et al.  Effects of chlorination and heat disinfection on long‐term starved Legionella pneumophila in warm water , 2007, Journal of applied microbiology.

[16]  F. Vandenesch,et al.  Integrated Real-Time PCR for Detection and Monitoring of Legionella pneumophila in Water Systems , 2006, Applied and Environmental Microbiology.

[17]  Anne K Camper,et al.  Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. , 2006, Journal of microbiological methods.

[18]  J. Kluytmans,et al.  Detection and quantification of Legionella pneumophila DNA in serum: case reports and review of the literature. , 2006, Journal of medical microbiology.

[19]  F. Vandenesch,et al.  Quantitative Real-Time Legionella PCR for Environmental Water Samples: Data Interpretation , 2006, Applied and Environmental Microbiology.

[20]  R. Ronimus,et al.  Rapid differentiation and enumeration of the total, viable vegetative cell and spore content of thermophilic bacilli in milk powders with reference to Anoxybacillus flavithermus , 2005, Journal of applied microbiology.

[21]  V. Pierzo,et al.  Rapid Method for Enumeration of Viable Legionella pneumophila and Other Legionella spp. in Water , 2005, Applied and Environmental Microbiology.

[22]  Knut Rudi,et al.  Use of Ethidium Monoazide and PCR in Combination for Quantification of Viable and Dead Cells in Complex Samples , 2005, Applied and Environmental Microbiology.

[23]  Carmen Pelaz,et al.  Legionnaires’ Disease Outbreak in Murcia, Spain , 2003, Emerging infectious diseases.

[24]  M. Pedro-Botet,et al.  Legionella spp.: community acquired and nosocomial infections , 2003, Current opinion in infectious diseases.

[25]  Barry S. Fields,et al.  Legionella and Legionnaires' Disease: 25 Years of Investigation , 2002, Clinical Microbiology Reviews.

[26]  P. Villari,et al.  Comparison of conventional culture and PCR methods for the detection of Legionella pneumophila in water , 1998, Letters in applied microbiology.

[27]  S. Yoshida,et al.  Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water , 1997, Applied and environmental microbiology.

[28]  R Amann,et al.  Resuscitation of viable but nonculturable Legionella pneumophila Philadelphia JR32 by Acanthamoeba castellanii , 1997, Applied and environmental microbiology.

[29]  M. von Knebel Doeberitz,et al.  Comparison of polymerase chain reaction and conventional culture for the detection of legionellas in hospital water samples. , 1994, The Journal of applied bacteriology.

[30]  R M Atlas,et al.  Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods , 1991, Applied and environmental microbiology.

[31]  R. Atlas,et al.  Detection of Legionella with polymerase chain reaction and gene probe methods. , 1990, Molecular and cellular probes.

[32]  Preben Aavitsland,et al.  An outbreak of legionnaires disease caused by long-distance spread from an industrial air scrubber in Sarpsborg, Norway. , 2008, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[33]  R. O'Loughlin,et al.  Surveillance for travel-associated legionnaires disease--United States, 2005-2006. , 2007, MMWR. Morbidity and mortality weekly report.

[34]  R. T. Porter Legionnaires' disease. , 1978, British medical journal.

[35]  G F Mallison,et al.  Legionnaires' disease: description of an epidemic of pneumonia. , 1977, The New England journal of medicine.